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AB240606

Anti-SHMT2/SHMT antibody

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(1 Publication)

Rabbit Polyclonal SHMT2/SHMT antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Serine hydroxymethyltransferase, mitochondrial aa 1-100.

View Alternative Names

SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT2

2 Images
Immunoprecipitation - Anti-SHMT2/SHMT antibody (AB240606)
  • IP

Supplier Data

Immunoprecipitation - Anti-SHMT2/SHMT antibody (AB240606)

SHMT2/SHMT was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer. For western blotting, ab240606 was used at 0.4 μg/ml.

Lane 1 : ab240606 used for IP at 6 μg per reaction.
Lane 2 : Control IgG IP.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-SHMT2/SHMT antibody (ab240606)

Predicted band size: 56 kDa

false

Western blot - Anti-SHMT2/SHMT antibody (AB240606)
  • WB

Supplier Data

Western blot - Anti-SHMT2/SHMT antibody (AB240606)

All lanes:

Western blot - Anti-SHMT2/SHMT antibody (ab240606) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 56 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human Serine hydroxymethyltransferase, mitochondrial aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P34897

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serine hydroxymethyltransferase 2 (SHMT2) also known as SHMT is an enzyme that plays a role in the mitochondrial compartment. It has a molecular mass of approximately 53 kDa. SHMT2 is highly expressed in tissues with elevated rates of oxidative metabolism such as the liver and brain. The enzyme catalyzes the reversible conversion of serine to glycine with the concomitant production of 510-methylenetetrahydrofolate a critical molecule for DNA synthesis and repair.
Biological function summary

The enzyme facilitates essential reactions in mitochondrial nucleotide synthesis. SHMT2 operates within a tetrameric complex that provides one-carbon units for cellular biosynthetic processes. The activity of SHMT2 links mitochondrial serine utilization directly to the folate cycle which is essential for the synthesis of purines and thymidylate. The efficient production of these nucleotides is important for rapidly dividing cells supporting their proliferation and maintenance.

Pathways

SHMT2 takes part in the folate and methionine metabolism pathways. It interacts with proteins like methylenetetrahydrofolate dehydrogenase and thymidylate synthase which are essential for one-carbon metabolism. These interactions integrate SHMT2 activity into a larger network that balances the supply of nucleotides according to the cellular demand impacting vital pathways like DNA replication and repair.

Elevated SHMT2 expression has been linked to cancer where it supports the rapid proliferation of tumor cells by providing necessary nucleotides. Its role in the mitochondrial folate pathway is also significant in neurodegenerative disorders. SHMT2 in conjunction with other proteins such as methionine synthase may play a role in the pathology of diseases like Alzheimer's by influencing homocysteine levels and oxidative stress in brain tissues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (PubMed : 24075985, PubMed : 25619277, PubMed : 29364879, PubMed : 33015733). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (PubMed : 25619277). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism : thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (PubMed : 21876188). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed : 29364879, PubMed : 29452640). Associates with mitochondrial DNA (PubMed : 18063578). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex : required for IFNAR1 deubiquitination by the BRISC complex (PubMed : 24075985).
See full target information Serine hydroxymethyltransferase, mitochondrial
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 32:420-436.e12 PubMed32768387

2020

Sirt2 Inhibition Enhances Metabolic Fitness and Effector Functions of Tumor-Reactive T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Imene Hamaidi,Lin Zhang,Nayoung Kim,Min-Hsuan Wang,Cristina Iclozan,Bin Fang,Min Liu,John M Koomen,Anders E Berglund,Sean J Yoder,Jiqiang Yao,Robert W Engelman,Ben C Creelan,Jose R Conejo-Garcia,Scott J Antonia,James J Mulé,Sungjune Kim
View all publications
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