JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB88664

Anti-SHMT2/SHMT antibody

4

(2 Reviews)

|

(1 Publication)

Mouse Polyclonal SHMT2/SHMT antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Serine hydroxymethyltransferase, mitochondrial.

View Alternative Names

SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody (AB88664)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody (AB88664)

ab88664, at 10 μg/ml, staining SHMT2/SHMT in Hela cells by Immunofluorescence.

Western blot - Anti-SHMT2/SHMT antibody (AB88664)
  • WB

Unknown

Western blot - Anti-SHMT2/SHMT antibody (AB88664)

All lanes:

Western blot - Anti-SHMT2/SHMT antibody (ab88664) at 1 µg/mL

All lanes:

Hela cell lysate at 50 µg

Predicted band size: 56 kDa

Observed band size: 42 kDa,56 kDa

false

Western blot - Anti-SHMT2/SHMT antibody (AB88664)
  • WB

Unknown

Western blot - Anti-SHMT2/SHMT antibody (AB88664)

All lanes:

Western blot - Anti-SHMT2/SHMT antibody (ab88664) at 1 µg/mL

Lane 1:

SHMT2/SHMT transfected 293T cell lysate at 25 µg

Lane 2:

Non transfected 293T cell lysate at 25 µg

Predicted band size: 56 kDa

Observed band size: 50 kDa

false

Western blot - Anti-SHMT2/SHMT antibody (AB88664)
  • WB

Unknown

Western blot - Anti-SHMT2/SHMT antibody (AB88664)

All lanes:

Western blot - Anti-SHMT2/SHMT antibody (ab88664) at 1 µg/mL

All lanes:

human spleen tissue lysate at 50 µg

Predicted band size: 56 kDa

Observed band size: 56 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Serine hydroxymethyltransferase, mitochondrial.

P34897

style
left-column

Alternative Products

Primary Antibodies

AB155230

Anti-SHMT2/SHMT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB155230)

  • 4
  • 1
Primary Antibodies

AB224428

Anti-SHMT2/SHMT antibody

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224428)

  • 0
  • 0
Primary Antibodies

AB224427

Anti-SHMT2/SHMT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224427)

  • 0
  • 0
Primary Antibodies

AB180786

Anti-SHMT2/SHMT antibody - C-terminal

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

  • 5
  • 2
Primary Antibodies

AB240606

Anti-SHMT2/SHMT antibody

Immunoprecipitation - Anti-SHMT2/SHMT antibody (AB240606)

  • 0
  • 0
Primary Antibodies

AB233430

Anti-SHMT2/SHMT antibody

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody (AB233430)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB32042

Anti-Cleaved Caspase-3 antibody [E83-77]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-Cleaved Caspase-3 antibody [E83-77] (AB32042)

  • 4
  • 28
Primary Antibodies

AB40772

Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (AB40772)

  • 4
  • 23
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serine hydroxymethyltransferase 2 (SHMT2) also known as SHMT is an enzyme that plays a role in the mitochondrial compartment. It has a molecular mass of approximately 53 kDa. SHMT2 is highly expressed in tissues with elevated rates of oxidative metabolism such as the liver and brain. The enzyme catalyzes the reversible conversion of serine to glycine with the concomitant production of 510-methylenetetrahydrofolate a critical molecule for DNA synthesis and repair.
Biological function summary

The enzyme facilitates essential reactions in mitochondrial nucleotide synthesis. SHMT2 operates within a tetrameric complex that provides one-carbon units for cellular biosynthetic processes. The activity of SHMT2 links mitochondrial serine utilization directly to the folate cycle which is essential for the synthesis of purines and thymidylate. The efficient production of these nucleotides is important for rapidly dividing cells supporting their proliferation and maintenance.

Pathways

SHMT2 takes part in the folate and methionine metabolism pathways. It interacts with proteins like methylenetetrahydrofolate dehydrogenase and thymidylate synthase which are essential for one-carbon metabolism. These interactions integrate SHMT2 activity into a larger network that balances the supply of nucleotides according to the cellular demand impacting vital pathways like DNA replication and repair.

Elevated SHMT2 expression has been linked to cancer where it supports the rapid proliferation of tumor cells by providing necessary nucleotides. Its role in the mitochondrial folate pathway is also significant in neurodegenerative disorders. SHMT2 in conjunction with other proteins such as methionine synthase may play a role in the pathology of diseases like Alzheimer's by influencing homocysteine levels and oxidative stress in brain tissues.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (PubMed : 24075985, PubMed : 25619277, PubMed : 29364879, PubMed : 33015733). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (PubMed : 25619277). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism : thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (PubMed : 21876188). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed : 29364879, PubMed : 29452640). Associates with mitochondrial DNA (PubMed : 18063578). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex : required for IFNAR1 deubiquitination by the BRISC complex (PubMed : 24075985).
See full target information Serine hydroxymethyltransferase, mitochondrial
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 36:2489-2500 PubMed27666119

2016

Prognostic and therapeutic value of mitochondrial serine hydroxyl-methyltransferase 2 as a breast cancer biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Lahong Zhang,Zhaojun Chen,Dan Xue,Qi Zhang,Xiyong Liu,Frank Luh,Liquan Hong,Hang Zhang,Feng Pan,Yuhua Liu,Peiguo Chu,Shu Zheng,Guoqiang Lou,Yun Yen
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com