AB180786

Anti-SHMT2/SHMT antibody - C-terminal

Name: Anti-SHMT2/SHMT antibody - C-terminal (ab180786) | Abcam
Brand: Abcam Limited
SKU: AB180786
Price: 545 USD
Availability: InStock
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(10 Publications)

Rabbit Polyclonal SHMT2/SHMT antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Serine hydroxymethyltransferase, mitochondrial aa 250 to C-terminus.

View Alternative Names

SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT2

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

Immunofluorescence staining of U-2 OS cells stained for SHMT2/SHMT with ab180786 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

Paraffin-embedded human liver cancer tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

Immunofluorescence staining of L929 cells stained for SHMT2/SHMT with ab180786 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

Paraffin-embedded rat spleen tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

Paraffin-embedded mouse spleen tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-SHMT2/SHMT antibody - C-terminal (ab180786) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 25 µg

Lane 2:

OVCAR3 cell lysate at 25 µg

Lane 3:

22Rv1 cell lysate at 25 µg

Lane 4:

Mouse liver tissue lysate at 25 µg

Lane 5:

Mouse kidney tissue lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L)

Predicted band size: 56 kDa

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Lab

Western blot - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

Western blot : Rabbit Polyclonal to SHMT2/SHMT ab180786 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 55 kDa in Wild-type A549 cell lysates with no signal observed at this size in SHMT2 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SHMT2/SHMT antibody - C-terminal (ab180786) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

Western blot - Human SHMT2 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-shmt2-knockout-a549-cell-line-ab301223'>ab301223</a>) at 20 µg

Lane 3:

HeLa

Secondary

Lanes 1 - 3:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 3:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 55 kDa,37 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Serine hydroxymethyltransferase, mitochondrial aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P34897

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serine hydroxymethyltransferase 2 (SHMT2) also known as SHMT is an enzyme that plays a role in the mitochondrial compartment. It has a molecular mass of approximately 53 kDa. SHMT2 is highly expressed in tissues with elevated rates of oxidative metabolism such as the liver and brain. The enzyme catalyzes the reversible conversion of serine to glycine with the concomitant production of 510-methylenetetrahydrofolate a critical molecule for DNA synthesis and repair.

Biological function summary

The enzyme facilitates essential reactions in mitochondrial nucleotide synthesis. SHMT2 operates within a tetrameric complex that provides one-carbon units for cellular biosynthetic processes. The activity of SHMT2 links mitochondrial serine utilization directly to the folate cycle which is essential for the synthesis of purines and thymidylate. The efficient production of these nucleotides is important for rapidly dividing cells supporting their proliferation and maintenance.

Pathways

SHMT2 takes part in the folate and methionine metabolism pathways. It interacts with proteins like methylenetetrahydrofolate dehydrogenase and thymidylate synthase which are essential for one-carbon metabolism. These interactions integrate SHMT2 activity into a larger network that balances the supply of nucleotides according to the cellular demand impacting vital pathways like DNA replication and repair.

Elevated SHMT2 expression has been linked to cancer where it supports the rapid proliferation of tumor cells by providing necessary nucleotides. Its role in the mitochondrial folate pathway is also significant in neurodegenerative disorders. SHMT2 in conjunction with other proteins such as methionine synthase may play a role in the pathology of diseases like Alzheimer's by influencing homocysteine levels and oxidative stress in brain tissues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (PubMed : 24075985, PubMed : 25619277, PubMed : 29364879, PubMed : 33015733). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (PubMed : 25619277). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism : thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (PubMed : 21876188). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed : 29364879, PubMed : 29452640). Associates with mitochondrial DNA (PubMed : 18063578). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex : required for IFNAR1 deubiquitination by the BRISC complex (PubMed : 24075985).

See full target information Serine hydroxymethyltransferase, mitochondrial

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadq1575 PubMed40184463

2025

Atf3 controls transitioning in female mitochondrial cardiomyopathy as identified by spatial and single-cell transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Tasneem Qaqorh,Yusuke Takahashi,Kohei Sameshima,Kentaro Otani,Issei Yazawa,Yuya Nishida,Kohei Tonai,Yoshitaka Fujihara,Mizuki Honda,Shinya Oki,Yasuyuki Ohkawa,David R Thorburn,Ann E Frazier,Atsuhito Takeda,Yoshihiko Ikeda,Heima Sakaguchi,Takuya Watanabe,Norihide Fukushima,Yasumasa Tsukamoto,Naomasa Makita,Osamu Yamaguchi,Kei Murayama,Akira Ohtake,Yasushi Okazaki,Takanari Kimura,Hisakazu Kato,Hijiri Inoue,Ken Matsuoka,Seiji Takashima,Yasunori Shintani

Cell death & disease 14:525 PubMed37582769

2023

Methionine orchestrates the metabolism vulnerability in cisplatin resistant bladder cancer microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Yang,Yuxi Ou,Quan Zhou,Yingchun Liang,Weijian Li,Yiling Chen,Wensun Chen,Siqi Wu,Yifan Chen,Xiyu Dai,Xinan Chen,Tian Chen,Shengming Jin,Yufei Liu,Limin Zhang,Shenghua Liu,Yun Hu,Lujia Zou,Shanhua Mao,Haowen Jiang

Cancer cell international 20:587 PubMed33372599

2020

RRM2 protects against ferroptosis and is a tumor biomarker for liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yueyue Yang,Jiafei Lin,Susu Guo,Xiangfei Xue,Yikun Wang,Shiyu Qiu,Jiangtao Cui,Lifang Ma,Xiao Zhang,Jiayi Wang

Cancer management and research 12:11169-11181 PubMed33173349

2020

Circ_0072995 Promotes Cell Carcinogenesis via Up-Regulating miR-149-5p-Mediated SHMT2 in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Qi,Xianxiong Qin,Zuozhi Zhou,Yan Wang,Qin Yang,Tianzhi Liao

Nan fang yi ke da xue xue bao = Journal of Southern Medical University 40:506-512 PubMed32895129

2020

[Protective effect of serine methyltransferase against hepatic ischemia-reperfusion injury in mice].

Applications

Unspecified application

Species

Unspecified reactive species

Yu Jiang,Ankang Wang,He Bai,Mingxin Ye

Cancers 12: PubMed32106628

2020

Reduced Expression Induces Chromosome Instability through Aberrant Cyclin E1 Protein Turnover.

Applications

Unspecified application

Species

Unspecified reactive species

Laura L Thompson,Allison K Baergen,Zelda Lichtensztejn,Kirk J McManus

Nature genetics 51:990-998 PubMed31133746

2019

MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Sdelci,André F Rendeiro,Philipp Rathert,Wanhui You,Jung-Ming G Lin,Anna Ringler,Gerald Hofstätter,Herwig P Moll,Bettina Gürtl,Matthias Farlik,Sandra Schick,Freya Klepsch,Matthew Oldach,Pisanu Buphamalai,Fiorella Schischlik,Peter Májek,Katja Parapatics,Christian Schmidl,Michael Schuster,Thomas Penz,Dennis L Buckley,Otto Hudecz,Richard Imre,Shuang-Yan Wang,Hans Michael Maric,Robert Kralovics,Keiryn L Bennett,Andre C Müller,Karl Mechtler,Jörg Menche,James E Bradner,Georg E Winter,Kristaps Klavins,Emilio Casanova,Christoph Bock,Johannes Zuber,Stefan Kubicek

American journal of cancer research 9:250-269 PubMed30906627

2019

MicroRNA-940 inhibits glioma progression by blocking mitochondrial folate metabolism through targeting of MTHFD2.

Applications

Unspecified application

Species

Unspecified reactive species

Tuoye Xu,Kaixin Zhang,Jing Shi,Baosheng Huang,Xi Wang,Kai Qian,Tao Ma,Tengda Qian,Zewu Song,Lixin Li

OncoTargets and therapy 10:3781-3788 PubMed28794642

2017

Overexpression of mitochondrial serine hydroxyl-methyltransferase 2 is associated with poor prognosis and promotes cell proliferation and invasion in gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Wu,Siyi Wanggou,Xuejun Li,Qing Liu,Yuanyang Xie

OncoTargets and therapy 8:1069-74 PubMed25999742

2015

Positive correlation between expression level of mitochondrial serine hydroxymethyltransferase and breast cancer grade.

Applications

IHC-P

Species

Human

Ke Yin

View all publications

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Product promise

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Anti-SHMT2/SHMT antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

Western blot - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

Western blot - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise