Rabbit Polyclonal SHMT2/SHMT antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SHMT2 aa 250 to C-terminus.
IgG
Rabbit
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Liquid
Polyclonal
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Tested | Tested | Tested |
Rat | Expected | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50.00000 - 1/200.00000 | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info 1/50.00000 - 1/200.00000 | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (PubMed:24075985, PubMed:29364879, PubMed:25619277). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (PubMed:25619277). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (PubMed:21876188). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed:29452640, PubMed:29364879). Associates with mitochondrial DNA (PubMed:18063578). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex: required for IFNAR1 deubiquitination by the BRISC complex (PubMed:24075985).
SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT2
Rabbit Polyclonal SHMT2/SHMT antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SHMT2 aa 250 to C-terminus.
SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT2
IgG
Rabbit
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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Serine hydroxymethyltransferase 2 (SHMT2) also known as SHMT is an enzyme that plays a role in the mitochondrial compartment. It has a molecular mass of approximately 53 kDa. SHMT2 is highly expressed in tissues with elevated rates of oxidative metabolism such as the liver and brain. The enzyme catalyzes the reversible conversion of serine to glycine with the concomitant production of 510-methylenetetrahydrofolate a critical molecule for DNA synthesis and repair.
The enzyme facilitates essential reactions in mitochondrial nucleotide synthesis. SHMT2 operates within a tetrameric complex that provides one-carbon units for cellular biosynthetic processes. The activity of SHMT2 links mitochondrial serine utilization directly to the folate cycle which is essential for the synthesis of purines and thymidylate. The efficient production of these nucleotides is important for rapidly dividing cells supporting their proliferation and maintenance.
SHMT2 takes part in the folate and methionine metabolism pathways. It interacts with proteins like methylenetetrahydrofolate dehydrogenase and thymidylate synthase which are essential for one-carbon metabolism. These interactions integrate SHMT2 activity into a larger network that balances the supply of nucleotides according to the cellular demand impacting vital pathways like DNA replication and repair.
Elevated SHMT2 expression has been linked to cancer where it supports the rapid proliferation of tumor cells by providing necessary nucleotides. Its role in the mitochondrial folate pathway is also significant in neurodegenerative disorders. SHMT2 in conjunction with other proteins such as methionine synthase may play a role in the pathology of diseases like Alzheimer's by influencing homocysteine levels and oxidative stress in brain tissues.
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Paraffin-embedded human liver cancer tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.
Immunofluorescence staining of L929 cells stained for SHMT2/SHMT with ab180786 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
Blocking buffer: 3% nonfat dry milk in TBST.
All lanes: Western blot - Anti-SHMT2/SHMT antibody - C-terminal (ab180786) at 1/1000 dilution
Lane 1: HeLa cell lysate at 25 µg
Lane 2: OVCAR3 cell lysate at 25 µg
Lane 3: 22Rv1 cell lysate at 25 µg
Lane 4: Mouse liver tissue lysate at 25 µg
Lane 5: Mouse kidney tissue lysate at 25 µg
All lanes: HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 56 kDa
Paraffin-embedded mouse spleen tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.
Immunofluorescence staining of U-2 OS cells stained for SHMT2/SHMT with ab180786 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
Paraffin-embedded rat spleen tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.
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