Rabbit Polyclonal SHMT2/SHMT antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Serine hydroxymethyltransferase, mitochondrial aa 250 to C-terminus.
Images
Publications
Filter by
- Cell death & disease 14:5252023Methionine orchestrates the metabolism vulnerability in cisplatin resistant bladder cancer microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Yang,Yuxi Ou,Quan Zhou,Yingchun Liang,Weijian Li,Yiling Chen,Wensun Chen,Siqi Wu,Yifan Chen,Xiyu Dai,Xinan Chen,Tian Chen,Shengming Jin,Yufei Liu,Limin Zhang,Shenghua Liu,Yun Hu,Lujia Zou,Shanhua Mao,Haowen JiangPubMed 37582769
- Cancer cell international 20:5872020RRM2 protects against ferroptosis and is a tumor biomarker for liver cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYueyue Yang et. al.PubMed 33372599
- Cancer management and research 12:11169-111812020Circ_0072995 Promotes Cell Carcinogenesis via Up-Regulating miR-149-5p-Mediated SHMT2 in Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuang Qi et. al.PubMed 33173349
- Cancers 12:2020Reduced Expression Induces Chromosome Instability through Aberrant Cyclin E1 Protein Turnover.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura L Thompson et. al.PubMed 32106628
- Nature genetics 51:990-9982019MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara Sdelci et. al.PubMed 31133746
- American journal of cancer research 9:250-2692019MicroRNA-940 inhibits glioma progression by blocking mitochondrial folate metabolism through targeting of MTHFD2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTuoye Xu et. al.PubMed 30906627
- OncoTargets and therapy 10:3781-37882017Overexpression of mitochondrial serine hydroxyl-methyltransferase 2 is associated with poor prognosis and promotes cell proliferation and invasion in gliomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Wu et. al.PubMed 28794642
- OncoTargets and therapy 8:1069-742015Positive correlation between expression level of mitochondrial serine hydroxymethyltransferase and breast cancer grade.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Serine hydroxymethyltransferase, mitochondrial aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P34897
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Tested |
| Rat | Expected | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50.00000 - 1/200.00000 | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info 1/50.00000 - 1/200.00000 | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (PubMed:24075985, PubMed:25619277, PubMed:29364879, PubMed:33015733). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (PubMed:25619277). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (PubMed:21876188). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed:29364879, PubMed:29452640). Associates with mitochondrial DNA (PubMed:18063578). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex: required for IFNAR1 deubiquitination by the BRISC complex (PubMed:24075985).
Alternative names
SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT2
Recommended products
Rabbit Polyclonal SHMT2/SHMT antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Serine hydroxymethyltransferase, mitochondrial aa 250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Serine hydroxymethyltransferase, mitochondrial aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P34897
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Serine hydroxymethyltransferase 2 (SHMT2) also known as SHMT is an enzyme that plays a role in the mitochondrial compartment. It has a molecular mass of approximately 53 kDa. SHMT2 is highly expressed in tissues with elevated rates of oxidative metabolism such as the liver and brain. The enzyme catalyzes the reversible conversion of serine to glycine with the concomitant production of 510-methylenetetrahydrofolate a critical molecule for DNA synthesis and repair.
- Biological function summary
The enzyme facilitates essential reactions in mitochondrial nucleotide synthesis. SHMT2 operates within a tetrameric complex that provides one-carbon units for cellular biosynthetic processes. The activity of SHMT2 links mitochondrial serine utilization directly to the folate cycle which is essential for the synthesis of purines and thymidylate. The efficient production of these nucleotides is important for rapidly dividing cells supporting their proliferation and maintenance.
- Pathways
SHMT2 takes part in the folate and methionine metabolism pathways. It interacts with proteins like methylenetetrahydrofolate dehydrogenase and thymidylate synthase which are essential for one-carbon metabolism. These interactions integrate SHMT2 activity into a larger network that balances the supply of nucleotides according to the cellular demand impacting vital pathways like DNA replication and repair.
- Associated diseases and disorders
Elevated SHMT2 expression has been linked to cancer where it supports the rapid proliferation of tumor cells by providing necessary nucleotides. Its role in the mitochondrial folate pathway is also significant in neurodegenerative disorders. SHMT2 in conjunction with other proteins such as methionine synthase may play a role in the pathology of diseases like Alzheimer's by influencing homocysteine levels and oxidative stress in brain tissues.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)
Paraffin-embedded human liver cancer tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)
Immunofluorescence staining of L929 cells stained for SHMT2/SHMT with ab180786 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
Western blot - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)
Blocking buffer: 3% nonfat dry milk in TBST.
All lanes: Western blot - Anti-SHMT2/SHMT antibody - C-terminal (ab180786) at 1/1000 dilution
Lane 1: HeLa cell lysate at 25 µg
Lane 2: OVCAR3 cell lysate at 25 µg
Lane 3: 22Rv1 cell lysate at 25 µg
Lane 4: Mouse liver tissue lysate at 25 µg
Lane 5: Mouse kidney tissue lysate at 25 µg
SecondaryAll lanes: HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)
Paraffin-embedded mouse spleen tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)
Immunofluorescence staining of U-2 OS cells stained for SHMT2/SHMT with ab180786 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)
Paraffin-embedded rat spleen tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com