AB316329

Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free

BOND RX™ Validated
Recombinant
RabMAb
KO Validated
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Rabbit Recombinant Monoclonal SHMT2/SHMT antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P, Flow Cyt (Intra), IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

View Alternative Names

SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT2

20 Images

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling SHMT2/SHMT with ab316328 at 1/1000 (0.5 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing mitochondrial staining in HeLa cell line.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling SHMT2/SHMT with ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : no staining on human skeletal muscle. The section was incubated with ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling SHMT2/SHMT with ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human colon carcinoma. The section was incubated with ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SHMT2/SHMT with ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human kidney (PMID : 33066813). The section was incubated with ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling SHMT2/SHMT with ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human hepatocellular carcinoma. The section was incubated with ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling SHMT2/SHMT with ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : no staining on human cardiac muscle. The section was incubated with ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Flow Cytometry (Intracellular) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling SHMT2/SHMT with ab316328 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

SHMT2/SHMT was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab316328 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316328 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab316328 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab316328 in HeLa whole cell lysate

All lanes:

Immunoprecipitation - Anti-SHMT2/SHMT antibody [EPR28607-51] (<a href='/en-us/products/primary-antibodies/shmt2-shmt-antibody-epr28607-51-ab316328'>ab316328</a>) at 1/30 dilution

All lanes:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 8s

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling SHMT2/SHMT with ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat liver. The section was incubated with ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling SHMT2/SHMT with ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : weak staining on mouse skeletal muscle. The section was incubated with ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling SHMT2/SHMT with ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : no staining on rat skeletal muscle. The section was incubated with ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling SHMT2/SHMT with ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse kidney. The section was incubated with ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling SHMT2/SHMT with ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse lung cancer. The section was incubated with ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling SHMT2/SHMT with ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat kidney. The section was incubated with ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SHMT2/SHMT with ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse liver. The section was incubated with ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labelling SHMT2/SHMT with ab316328 at 1/1000 (0.5 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing mitochondrial staining in Hepa1-6 cell line.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

Supplier Data

Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

Low expression : skeletal muscle

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] (<a href='/en-us/products/primary-antibodies/shmt2-shmt-antibody-epr28607-51-ab316328'>ab316328</a>) at 1/5000 dilution

Lane 1:

Hela (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting SHMT2 whole cell lysate at 20 µg

Lane 3:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Mouse kidney tissue lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Lane 6:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 7:

Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 55 kDa,124 kDa

false

Exposure time: 26s

Supplier Data

Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] (<a href='/en-us/products/primary-antibodies/shmt2-shmt-antibody-epr28607-51-ab316328'>ab316328</a>) at 1/5000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Rat liver tissue lysate at 20 µg

Lane 4:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 55 kDa

false

Exposure time: 6s

Lab

Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

Western blot : Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 staining at 1/5000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 55 kDa in Wild-type A549 cell lysates with no signal observed at this size in SHMT2 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution."

All lanes:

Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] (<a href='/en-us/products/primary-antibodies/shmt2-shmt-antibody-epr28607-51-ab316328'>ab316328</a>) at 1/5000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

Western blot - Human SHMT2 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-shmt2-knockout-a549-cell-line-ab301223'>ab301223</a>) at 20 µg

Lane 3:

HeLa at 20 µg

Secondary

Lanes 1 - 3:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 3:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 55 kDa,37 kDa

false

Supplier Data

Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

This data was developed using ab316328, the same antibody clone in a different buffer formulation.

This antibody does not cross-react with human SHMT1.

In Western blot, Anti-6X His tag antibody [EPR20547]-CHIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] (<a href='/en-us/products/primary-antibodies/shmt2-shmt-antibody-epr28607-51-ab316328'>ab316328</a>) at 1/5000 dilution

Lane 1:

His-tagged human SHMT2 recombinant protein at 10 ng

Lane 2:

His-tagged human SHMT1 recombinant protein at 10 ng

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution

Observed band size: 55 kDa

false

Exposure time: 1s

Unconjugated

Anti-SHMT2/SHMT antibody [EPR28607-51]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28607-51

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, IHC-P, Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

ab316329 is the carrirer-free version of ab316328.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serine hydroxymethyltransferase 2 (SHMT2) also known as SHMT is an enzyme that plays a role in the mitochondrial compartment. It has a molecular mass of approximately 53 kDa. SHMT2 is highly expressed in tissues with elevated rates of oxidative metabolism such as the liver and brain. The enzyme catalyzes the reversible conversion of serine to glycine with the concomitant production of 510-methylenetetrahydrofolate a critical molecule for DNA synthesis and repair.

Biological function summary

The enzyme facilitates essential reactions in mitochondrial nucleotide synthesis. SHMT2 operates within a tetrameric complex that provides one-carbon units for cellular biosynthetic processes. The activity of SHMT2 links mitochondrial serine utilization directly to the folate cycle which is essential for the synthesis of purines and thymidylate. The efficient production of these nucleotides is important for rapidly dividing cells supporting their proliferation and maintenance.

Pathways

SHMT2 takes part in the folate and methionine metabolism pathways. It interacts with proteins like methylenetetrahydrofolate dehydrogenase and thymidylate synthase which are essential for one-carbon metabolism. These interactions integrate SHMT2 activity into a larger network that balances the supply of nucleotides according to the cellular demand impacting vital pathways like DNA replication and repair.

Elevated SHMT2 expression has been linked to cancer where it supports the rapid proliferation of tumor cells by providing necessary nucleotides. Its role in the mitochondrial folate pathway is also significant in neurodegenerative disorders. SHMT2 in conjunction with other proteins such as methionine synthase may play a role in the pathology of diseases like Alzheimer's by influencing homocysteine levels and oxidative stress in brain tissues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (PubMed : 24075985, PubMed : 25619277, PubMed : 29364879, PubMed : 33015733). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (PubMed : 25619277). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism : thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (PubMed : 21876188). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed : 29364879, PubMed : 29452640). Associates with mitochondrial DNA (PubMed : 18063578). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex : required for IFNAR1 deubiquitination by the BRISC complex (PubMed : 24075985).

See full target information Serine hydroxymethyltransferase, mitochondrial

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Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Flow Cytometry (Intracellular) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Immunoprecipitation - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

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