Rabbit Recombinant Monoclonal SHMT2/SHMT antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P, Flow Cyt (Intra), IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
Images
![Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--western-blot-img427397.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)")
![Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--western-blot-img427395.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)")
![Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--western-blot-img427396.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)")
![Flow Cytometry (Intracellular) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--flow-cytometry-intracellular-img427517.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)")
![Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunocytochemistry-immunofluorescence-img427506.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Tested | Tested | Tested | Expected | Expected |
| Rat | Tested | Expected | Tested | Expected | Expected |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (PubMed:24075985, PubMed:29364879, PubMed:25619277). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (PubMed:25619277). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (PubMed:21876188). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed:29452640, PubMed:29364879). Associates with mitochondrial DNA (PubMed:18063578). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex: required for IFNAR1 deubiquitination by the BRISC complex (PubMed:24075985).
Alternative names
SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT2
Recommended products
Rabbit Recombinant Monoclonal SHMT2/SHMT antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P, Flow Cyt (Intra), IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR28607-51
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab316329 is the carrirer-free version of Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Serine hydroxymethyltransferase 2 (SHMT2) also known as SHMT is an enzyme that plays a role in the mitochondrial compartment. It has a molecular mass of approximately 53 kDa. SHMT2 is highly expressed in tissues with elevated rates of oxidative metabolism such as the liver and brain. The enzyme catalyzes the reversible conversion of serine to glycine with the concomitant production of 510-methylenetetrahydrofolate a critical molecule for DNA synthesis and repair.
- Biological function summary
The enzyme facilitates essential reactions in mitochondrial nucleotide synthesis. SHMT2 operates within a tetrameric complex that provides one-carbon units for cellular biosynthetic processes. The activity of SHMT2 links mitochondrial serine utilization directly to the folate cycle which is essential for the synthesis of purines and thymidylate. The efficient production of these nucleotides is important for rapidly dividing cells supporting their proliferation and maintenance.
- Pathways
SHMT2 takes part in the folate and methionine metabolism pathways. It interacts with proteins like methylenetetrahydrofolate dehydrogenase and thymidylate synthase which are essential for one-carbon metabolism. These interactions integrate SHMT2 activity into a larger network that balances the supply of nucleotides according to the cellular demand impacting vital pathways like DNA replication and repair.
- Associated diseases and disorders
Elevated SHMT2 expression has been linked to cancer where it supports the rapid proliferation of tumor cells by providing necessary nucleotides. Its role in the mitochondrial folate pathway is also significant in neurodegenerative disorders. SHMT2 in conjunction with other proteins such as methionine synthase may play a role in the pathology of diseases like Alzheimer's by influencing homocysteine levels and oxidative stress in brain tissues.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
19 product images
![Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--western-blot-img427397.jpg "Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
This antibody does not cross-react with human SHMT1.
In Western blot, Anti-6X His tag antibody [EPR20547]-CHIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] (Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328) at 1/5000 dilution
Lane 1: His-tagged human SHMT2 recombinant protein at 10 ng
Lane 2: His-tagged human SHMT1 recombinant protein at 10 ng
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution
Observed band size: 55 kDa
Exposure time: 1s
![Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--western-blot-img427395.jpg "Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
Low expression: skeletal muscle
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] (Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328) at 1/5000 dilution
Lane 1: Hela (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: HeLa transfected with siRNA specifically targeting SHMT2 whole cell lysate at 20 µg
Lane 3: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: Mouse kidney tissue lysate at 20 µg
Lane 5: Mouse liver tissue lysate at 20 µg
Lane 6: Mouse skeletal muscle tissue lysate at 20 µg
Lane 7: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 55 kDa, 124 kDa
Exposure time: 26s
Low expression: skeletal muscle
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
![Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--western-blot-img427396.jpg "Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] (Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328) at 1/5000 dilution
Lane 1: Human kidney tissue lysate at 20 µg
Lane 2: Human liver tissue lysate at 20 µg
Lane 3: Rat liver tissue lysate at 20 µg
Lane 4: Rat kidney tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 55 kDa
Exposure time: 6s
![Flow Cytometry (Intracellular) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--flow-cytometry-intracellular-img427517.jpg "Flow Cytometry (Intracellular) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Flow Cytometry (Intracellular) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling SHMT2/SHMT with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunocytochemistry-immunofluorescence-img427506.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labelling SHMT2/SHMT with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/1000 (0.5 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing mitochondrial staining in Hepa1-6 cell line.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunocytochemistry-immunofluorescence-img427505.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling SHMT2/SHMT with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/1000 (0.5 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing mitochondrial staining in HeLa cell line.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427460.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling SHMT2/SHMT with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: no staining on rat skeletal muscle. The section was incubated with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427459.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling SHMT2/SHMT with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: weak staining on mouse skeletal muscle. The section was incubated with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427458.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling SHMT2/SHMT with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: no staining on human cardiac muscle. The section was incubated with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427457.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling SHMT2/SHMT with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: no staining on human skeletal muscle. The section was incubated with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427456.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling SHMT2/SHMT with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat liver. The section was incubated with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427455.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling SHMT2/SHMT with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat kidney. The section was incubated with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427454.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling SHMT2/SHMT with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse lung cancer. The section was incubated with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427453.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SHMT2/SHMT with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse liver. The section was incubated with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427452.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling SHMT2/SHMT with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse kidney. The section was incubated with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427451.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling SHMT2/SHMT with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human colon carcinoma. The section was incubated with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427450.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling SHMT2/SHMT with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human hepatocellular carcinoma. The section was incubated with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427449.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SHMT2/SHMT with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human kidney (PMID: 33066813). The section was incubated with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunoprecipitation - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunoprecipitation-img427439.jpg "Immunoprecipitation - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
Immunoprecipitation - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)
This data was developed using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328, the same antibody clone in a different buffer formulation.
SHMT2/SHMT was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2: Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 in HeLa whole cell lysateAll lanes: Immunoprecipitation - Anti-SHMT2/SHMT antibody [EPR28607-51] (Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328) at 1/30 dilution
All lanes: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 8s
SHMT2/SHMT was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2: Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 in HeLa whole cell lysate
![Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunocytochemistry-immunofluorescence-img427505.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427460.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427459.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427458.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427457.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427456.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427455.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427454.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427453.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427452.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427451.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427450.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427449.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
![Immunoprecipitation - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329), expandable thumbnail](https://content.abcam.com/products/images/shmt2-shmt-antibody-epr28607-51-bsa-and-azide-free-ab316329--immunoprecipitation-img427439.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (ab316329)")
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