JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB229805

Anti-Shoc2 / Sur8 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal Shoc2 / Sur8 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SHOC2 aa 1-100.

View Alternative Names

KIAA0862, SHOC2, Leucine-rich repeat protein SHOC-2, Protein soc-2 homolog, Protein sur-8 homolog

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Shoc2 / Sur8 antibody (AB229805)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Shoc2 / Sur8 antibody (AB229805)

Paraffin-embedded human lung tissue stained for Shoc2 / Sur8 with ab229805 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Shoc2 / Sur8 antibody (AB229805)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Shoc2 / Sur8 antibody (AB229805)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Shoc2 / Sur8 (green) using ab229805 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Shoc2 / Sur8 antibody (AB229805)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Shoc2 / Sur8 antibody (AB229805)

Paraffin-embedded human brain tissue stained for Shoc2 / Sur8 with ab229805 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-Shoc2 / Sur8 antibody (AB229805)
  • WB

Supplier Data

Western blot - Anti-Shoc2 / Sur8 antibody (AB229805)

All lanes:

Western blot - Anti-Shoc2 / Sur8 antibody (ab229805) at 1/500 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 64 kDa

Observed band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SHOC2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9UQ13

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates activation of the MAPK pathway (PubMed : 10783161, PubMed : 16630891, PubMed : 25137548, PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670). Acts as a scaffolding protein in the SMP complex (PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed : 10783161, PubMed : 16630891, PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670). The SMP complex enhances the dephosphorylation activity and substrate specificity of PP1c (PubMed : 35768504, PubMed : 36175670).
See full target information SHOC2
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Aging 15:3759-3770 PubMed37166421

2023

LncRNA FALEC increases the proliferation, migration and drug resistance of cholangiocarcinoma through competitive regulation of miR-20a-5p/SHOC2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Haiming Du,Senlin Hou,Lichao Zhang,Chao Liu,Tingting Yu,Wei Zhang

International journal of biological sciences 18:5168-5184 PubMed35982894

2022

Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Ni,Guo-Hua Wu,Juan-Juan Cai,Run Zhang,Yang Zheng,Jing-Quan Liu,Xiang-Hong Yang,Xue Yang,Ye Shen,Jun-Mei Lai,Xiang-Ming Ye,Shi-Jing Mo
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB229315

Anti-Shoc2 / Sur8 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Shoc2 / Sur8 antibody - C-terminal (AB229315)

  • 0
  • 0
Primary Antibodies

AB106430

Anti-Shoc2 / Sur8 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Shoc2 / Sur8 antibody (AB106430)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB229912

Anti-PERK antibody [EPR19876-294]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-PERK antibody [EPR19876-294] (AB229912)

  • 4
  • 5
Primary Antibodies

AB184699

Anti-ERK1 + ERK2 antibody [EPR17526]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (AB184699)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com