Mouse Monoclonal SHOX2 antibody. Suitable for WB, IHC-Fr and reacts with Recombinant fragment - Human, Rat, Human samples. Cited in 17 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SHOX2 aa 100-250.
Images
![Western blot - Anti-SHOX2 antibody [1D1] (AB55740), expandable thumbnail](https://content.abcam.com/products/images/shox2-antibody-1d1-ab55740--western-blot-img150443.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SHOX2 antibody [1D1] (AB55740)")
![Western blot - Anti-SHOX2 antibody [1D1] (AB55740), expandable thumbnail](https://content.abcam.com/products/images/shox2-antibody-1d1-ab55740--western-blot-img64515.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SHOX2 antibody [1D1] (AB55740)")
Publications
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- Biology open 12:2023Tbx5 overexpression in embryoid bodies increases TAK1 expression but does not enhance the differentiation of sinoatrial node cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunkai Dai,Fatemeh Nasehi,Charles D Winchester,Ann C FoleyPubMed 37272627
- Journal of tissue engineering 13:204173142211279082022Molecular and electrophysiological evaluation of human cardiomyocyte subtypes to facilitate generation of composite cardiac models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiuru Li,Alexandra Wiesinger,Lianne Fokkert,Bastiaan J Boukens,Arie O Verkerk,Vincent M Christoffels,Gerard J J Boink,Harsha D DevallaPubMed 36277058
- eLife 11:2022A single cell transcriptional roadmap of human pacemaker cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra Wiesinger,Jiuru Li,Lianne Fokkert,Priscilla Bakker,Arie O Verkerk,Vincent M Christoffels,Gerard J J Boink,Harsha D DevallaPubMed 36217819
- Stem cell research & therapy 13:3242022RA signaling pathway combined with Wnt signaling pathway regulates human-induced pluripotent stem cells (hiPSCs) differentiation to sinus node-like cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Yin,Feng-Yuan Wang,Wei Zhang,Xi Wang,Yan-Hong Tang,Teng Wang,Yu-Ting Chen,Cong-Xin HuangPubMed 35851424
- International journal of stem cells 15:247-2572022The Biphasic Effect of Retinoic Acid Signaling Pathway on the Biased Differentiation of Atrial-like and Sinoatrial Node-like Cells from hiPSC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Liu,Dandan Long,Wenjun Huang,Wanling Peng,Huan Lan,Yafei Zhou,Xitong Dang,Rui ZhouPubMed 35220280
- Genesis (New York, N.Y. : 2000) 59:e234352021Generation and characterization of a tamoxifen-inducible Vsx1-CreER line to target V2 interneurons in the mouse developing spinal cord.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharlotte Baudouin,Barbara Pelosi,Guillaume E Courtoy,Younes Achouri,Frédéric ClotmanPubMed 34080769
- Stem cell research 49:1020432020NODAL inhibition promotes differentiation of pacemaker-like cardiomyocytes from human induced pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSergey Yechikov,Hillary K J Kao,Che-Wei Chang,Dalyir Pretto,Xiao-Dong Zhang,Yao-Hui Sun,Regan Smithers,Padmini Sirish,Jan A Nolta,James W Chan,Nipavan Chiamvimonvat,Deborah K LieuPubMed 33128951
- Journal of orthopaedic research : official publication of the Orthopaedic Research Society 39:1745-17542020TNF-α suppresses SHOX2 expression via NF-κB signaling pathway and promotes intervertebral disc degeneration and related pain in a rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFubiao Ye,Yang Xu,Feiyue Lin,Zhaomin ZhengPubMed 32816304
- Stem cell research & therapy 11:2842020Enrichment differentiation of human induced pluripotent stem cells into sinoatrial node-like cells by combined modulation of BMP, FGF, and RA signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Liu et. al.PubMed 32678003
- Scientific reports 10:9962020Onecut-dependent Nkx6.2 transcription factor expression is required for proper formation and activity of spinal locomotor circuits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMathilde Toch et. al.PubMed 31969659
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human SHOX2 aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link O60902
Reactivity data
Select an application| WB | IHC-Fr | |
|---|---|---|
| Human | Predicted | Expected |
| Rat | Tested | Expected |
| Recombinant fragment - Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Species Rat | Dilution info 1.00000-5.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
May be a growth regulator and have a role in specifying neural systems involved in processing somatosensory information, as well as in face and body structure formation.
Alternative names
OG12X, SHOT, SHOX2, Short stature homeobox protein 2, Homeobox protein Og12X, Paired-related homeobox protein SHOT
Recommended products
Mouse Monoclonal SHOX2 antibody. Suitable for WB, IHC-Fr and reacts with Recombinant fragment - Human, Rat, Human samples. Cited in 17 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SHOX2 aa 100-250.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human SHOX2 aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link O60902
- Clone number
- 1D1
- Purity
- Tissue culture supernatant
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 16/Oct/2019. Lot numbers higher than GR3303529 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The short stature homeobox 2 (SHOX2) is a transcription factor protein involved in various regulatory functions. It is also known as OG12X and is characterized by its homeobox domain. SHOX2 weighs approximately 28 kDa. Its expression occurs mainly in the embryonic heart skeletal system and developing limbs. Researchers observe SHOX2 in specific adult tissues such as the sinoatrial node of the heart where it plays roles in maintaining regular cardiac rhythm.
- Biological function summary
The protein influences developmental processes by regulating gene expression during embryogenesis. SHOX2 is not part of a large complex but functions independently to modulate the development of tissues and organs. It ensures proper formation of heart structures skeletal growth and limb patterning. SHOX2's absence or malfunction can lead to disruptions in the structural development of these areas.
- Pathways
SHOX2 has significant roles in cardiac pacemaker and limb development pathways. It is heavily involved in the canonical Wnt signaling pathway which supports the growth and differentiation of various tissues. The relationship between SHOX2 and BMP4 another protein in this pathway is important for bone development and limb formation. SHOX2 also interfaces with T-box proteins like TBX5 contributing to the specification and function of heart tissues.
- Associated diseases and disorders
SHOX2 links to conditions such as short stature homeobox-related syndromes and congenital heart defects. Dysfunctions in SHOX2 can lead to Léri-Weill dyschondrosteosis a disorder associated with short stature and skeletal abnormalities. In heart development the interaction between SHOX2 and NKX2-5 another important transcription factor is pivotal. Abnormalities or mutations in SHOX2 may also contribute to sinus node dysfunction impacting heart rate regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-SHOX2 antibody [1D1] (ab55740), expandable thumbnail](https://content.abcam.com/products/images/shox2-antibody-1d1-ab55740--western-blot-img150443.jpg "Western blot - Anti-SHOX2 antibody [1D1] (ab55740)")
Western blot - Anti-SHOX2 antibody [1D1] (ab55740)
SHOX2 Western blot staining of Recombinant SHOX2 Protein using mouse Anti-SHOX2 antibody
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-SHOX2 antibody [1D1] (ab55740)
All lanes: Recombinant SHOX2 Protein
Predicted band size: 34 kDa
![Western blot - Anti-SHOX2 antibody [1D1] (ab55740), expandable thumbnail](https://content.abcam.com/products/images/shox2-antibody-1d1-ab55740--western-blot-img64515.jpg "Western blot - Anti-SHOX2 antibody [1D1] (ab55740)")
Western blot - Anti-SHOX2 antibody [1D1] (ab55740)
SHOX2 antibody (ab55740) at 1ug/lane + PC-12 cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-SHOX2 antibody [1D1] (ab55740)
Predicted band size: 34 kDa
Downloads
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