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AB131537

Anti-SHP1 antibody

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(5 Publications)

Rabbit Polyclonal SHP1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PTPN6 aa 500-550 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

HCP, PTP1C, PTPN6, Tyrosine-protein phosphatase non-receptor type 6, Hematopoietic cell protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1, SH-PTP1, PTP-1C

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SHP1 antibody (AB131537)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SHP1 antibody (AB131537)

Immunofluorescence analysis of methanol-fixed MCF cells labelling SHP1 with ab131537 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody (AB131537)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody (AB131537)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling SHP1 with ab131537 at 1/50 dilution. Right panel was preincubated with blocking peptide.

Western blot - Anti-SHP1 antibody (AB131537)
  • WB

Unknown

Western blot - Anti-SHP1 antibody (AB131537)

All lanes:

Western blot - Anti-SHP1 antibody (ab131537) at 1/500 dilution

All lanes:

293 cell extract

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PTPN6 aa 500-550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P29350

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHP1 also known as PTPN6 belongs to the family of protein tyrosine phosphatases and has a protein mass of approximately 68 kDa. It plays a critical role in cell signaling by dephosphorylating specific targets leading to the modulation of signal transduction pathways. SHP1 is ubiquitously expressed with high levels observed in hematopoietic cells suggesting a specialized function in the immune system.
Biological function summary

SHP1 influences cell proliferation survival and differentiation by acting as a negative regulator of signaling pathways. It is not a part of a larger protein complex but it associates transiently with different receptors and substrates. It plays an important role in the immune response by regulating cytokine signaling which is important for maintaining the balance between immune activation and tolerance.

Pathways

SHP1 significantly impacts the JAK/STAT and B cell receptor signaling pathways. In the JAK/STAT pathway SHP1 interacts with JAK kinases contributing to the downregulation of cytokine signaling. In B cell receptor signaling it associates with Syk influencing immune cell activation and function.

SHP1 has a role in autoimmune diseases such as rheumatoid arthritis and certain types of leukemia. In rheumatoid arthritis defective regulation by SHP1 can lead to excessive inflammation while in leukemia its altered activity impacts cell growth and survival. It interacts with proteins such as STATs and SOCS which are important in these pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine phosphatase enzyme that plays important roles in controlling immune signaling pathways and fundamental physiological processes such as hematopoiesis (PubMed : 14739280, PubMed : 29925997). Dephosphorylates and negatively regulate several receptor tyrosine kinases (RTKs) such as EGFR, PDGFR and FGFR, thereby modulating their signaling activities (PubMed : 21258366, PubMed : 9733788). When recruited to immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing receptors such as immunoglobulin-like transcript 2/LILRB1, programmed cell death protein 1/PDCD1, CD3D, CD22, CLEC12A and other receptors involved in immune regulation, initiates their dephosphorylation and subsequently inhibits downstream signaling events (PubMed : 11907092, PubMed : 14739280, PubMed : 37932456, PubMed : 38166031). Modulates the signaling of several cytokine receptors including IL-4 receptor (PubMed : 9065461). Additionally, targets multiple cytoplasmic signaling molecules including STING1, LCK or STAT1 among others involved in diverse cellular processes including modulation of T-cell activation or cGAS-STING signaling (PubMed : 34811497, PubMed : 38532423). Within the nucleus, negatively regulates the activity of some transcription factors such as NFAT5 via direct dephosphorylation. Also acts as a key transcriptional regulator of hepatic gluconeogenesis by controlling recruitment of RNA polymerase II to the PCK1 promoter together with STAT5A (PubMed : 37595871).
See full target information PTPN6
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112266 PubMed40241752

2025

CRISPR/Cas9-mediated SHP-1-knockout T cells combined with simvastatin enhances anti-tumor activity in humanized-PDX HCC model.

Applications

Unspecified application

Species

Unspecified reactive species

Huaping Liu,Wu Ge,Xiaoping Yu,Jianwei Luo,Juan Zhang,Min Yang,Lu Cao,Yangnan Zhang,Ruike Wang,Cejun Yang,Pei Li,Mengyu Tian,XiaoPei Peng,Lei Peng,Di Wu,Muqi Liu,Qi Liang,Shengwang Zhang,Wei Li,Pengfei Rong,Hailan Li,Xiaoqian Ma,Wei Wang

Communications biology 7:1073 PubMed39217250

2024

Reversible control of kinase signaling through chemical-induced dephosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Sun,Rihong Zhou,Jin Hu,Shan Feng,Qi Hu

The Journal of biological chemistry 294:11910-11919 PubMed31201275

2019

The female reproductive tract contains multiple innate sialic acid-binding immunoglobulin-like lectins (Siglecs) that facilitate sperm survival.

Applications

Unspecified application

Species

Unspecified reactive species

Eillen Tecle,Hector Sequoyah Reynoso,Ruixuan Wang,Pascal Gagneux

The Journal of experimental medicine 215:1337-1347 PubMed29669741

2018

SHP-1 regulates hematopoietic stem cell quiescence by coordinating TGF-β signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Linjia Jiang,Xue Han,Jin Wang,Chen Wang,Xiaoqiang Sun,Jiayi Xie,Guojin Wu,Hiep Phan,Zhenguo Liu,Edward T H Yeh,ChengCheng Zhang,Meng Zhao,Xunlei Kang

FASEB journal : official publication of the Federa 31:2001-2012 PubMed28183800

2017

TNFSF15 inhibits VEGF-stimulated vascular hyperpermeability by inducing VEGFR2 dephosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Gui-Li Yang,Zilong Zhao,Ting-Ting Qin,Dong Wang,Lijuan Chen,Rong Xiang,Zhen Xi,Rongcai Jiang,Zhi-Song Zhang,Jianning Zhang,Lu-Yuan Li
View all publications
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Product promise

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