Rabbit Recombinant Monoclonal SHP1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Images
![Western blot - Anti-SHP1 antibody [Y476] - BSA and Azide free (AB226008), expandable thumbnail](https://content.abcam.com/products/images/shp1-antibody-y476-bsa-and-azide-free-ab226008--western-blot-img136540.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SHP1 antibody [Y476] - BSA and Azide free (AB226008)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (AB226008), expandable thumbnail](https://content.abcam.com/products/images/shp1-antibody-y476-bsa-and-azide-free-ab226008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86421.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (AB226008)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (AB226008), expandable thumbnail](https://content.abcam.com/products/images/shp1-antibody-y476-bsa-and-azide-free-ab226008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126606.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (AB226008)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (AB226008), expandable thumbnail](https://content.abcam.com/products/images/shp1-antibody-y476-bsa-and-azide-free-ab226008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58002.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (AB226008)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (AB226008), expandable thumbnail](https://content.abcam.com/products/images/shp1-antibody-y476-bsa-and-azide-free-ab226008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70091.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (AB226008)")
Publications
Filter by
- Journal of molecular medicine (Berlin, Germany) 92:983-942014Inhibition of protein tyrosine phosphatases enhances cerebral collateral growth in rats.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
12 products for Alternative Product
2 products for Alternative Version
Target data
Function
Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.
Alternative names
Tyrosine-protein phosphatase non-receptor type 6, Hematopoietic cell protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1, SH-PTP1, PTP-1C, PTPN6, HCP, PTP1C
Recommended products
Rabbit Recombinant Monoclonal SHP1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Alternative names
Tyrosine-protein phosphatase non-receptor type 6, Hematopoietic cell protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1, SH-PTP1, PTP-1C, PTPN6, HCP, PTP1C
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
Y476
- Purification technique
Affinity purification Protein A
- Specificity
The antibody is predicted to detect isoforms 1, 2 and 3 of human SHP1 based on sequence analysis.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab226008 is the carrier-free version of Anti-SHP1 antibody [Y476] ab32559.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SHP1 also known as PTPN6 belongs to the family of protein tyrosine phosphatases and has a protein mass of approximately 68 kDa. It plays a critical role in cell signaling by dephosphorylating specific targets leading to the modulation of signal transduction pathways. SHP1 is ubiquitously expressed with high levels observed in hematopoietic cells suggesting a specialized function in the immune system.
- Biological function summary
SHP1 influences cell proliferation survival and differentiation by acting as a negative regulator of signaling pathways. It is not a part of a larger protein complex but it associates transiently with different receptors and substrates. It plays an important role in the immune response by regulating cytokine signaling which is important for maintaining the balance between immune activation and tolerance.
- Pathways
SHP1 significantly impacts the JAK/STAT and B cell receptor signaling pathways. In the JAK/STAT pathway SHP1 interacts with JAK kinases contributing to the downregulation of cytokine signaling. In B cell receptor signaling it associates with Syk influencing immune cell activation and function.
- Associated diseases and disorders
SHP1 has a role in autoimmune diseases such as rheumatoid arthritis and certain types of leukemia. In rheumatoid arthritis defective regulation by SHP1 can lead to excessive inflammation while in leukemia its altered activity impacts cell growth and survival. It interacts with proteins such as STATs and SOCS which are important in these pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008), expandable thumbnail](https://content.abcam.com/products/images/shp1-antibody-y476-bsa-and-azide-free-ab226008--western-blot-img136540.jpg "Western blot - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008)")
Western blot - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008)
This data was developed using the same antibody clone in a different buffer formulation (Anti-SHP1 antibody [Y476] ab32559).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-SHP1 antibody [Y476] ab32559 observed at 70 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
Anti-SHP1 antibody [Y476] ab32559 was shown to react with SHP1 in wild-type THP-1 cells in Western blot with loss of signal observed in PTPN6 knockout sample. Wild-type THP-1 and PTPN6 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with Anti-SHP1 antibody [Y476] ab32559 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-SHP1 antibody [Y476] (Anti-SHP1 antibody [Y476] ab32559) at 1/1000 dilution
Lane 1: Wild-type THP-1 cell lysate at 20 µg
Lane 2: PTPN6 knockout THP-1 cell lysate at 20 µg
Lane 3: Jurkat cell lysate at 20 µg
Lane 4: K562 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 68 kDa, 83 kDa
Observed band size: 70 kDa, 85 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008), expandable thumbnail](https://content.abcam.com/products/images/shp1-antibody-y476-bsa-and-azide-free-ab226008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86421.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008)
Immunohistochemical staining of paraffin embedded rat spleen with purified Anti-SHP1 antibody [Y476] ab32559 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SHP1 antibody [Y476] ab32559).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008), expandable thumbnail](https://content.abcam.com/products/images/shp1-antibody-y476-bsa-and-azide-free-ab226008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126606.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008)
Immunohistochemical staining of paraffin embedded mouse liver with purified Anti-SHP1 antibody [Y476] ab32559 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SHP1 antibody [Y476] ab32559).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008), expandable thumbnail](https://content.abcam.com/products/images/shp1-antibody-y476-bsa-and-azide-free-ab226008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58002.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008)
Immunohistochemical staining of paraffin embedded human tonsil with purified Anti-SHP1 antibody [Y476] ab32559 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SHP1 antibody [Y476] ab32559).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008), expandable thumbnail](https://content.abcam.com/products/images/shp1-antibody-y476-bsa-and-azide-free-ab226008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70091.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008)
This IHC data was generated using the same anti-SHP1 antibody clone [Y476] in a different buffer formulation (cat# Anti-SHP1 antibody [Y476] ab32559).
Unpurified Anti-SHP1 antibody [Y476] ab32559, at a 1/50 dilution, staining human lymph node by immunohistochemistry, Paraffin embedded tissue.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008), expandable thumbnail](https://content.abcam.com/products/images/shp1-antibody-y476-bsa-and-azide-free-ab226008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423106.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008)
Tissue Microarrays stained for "Anti-SHP1 antibody [Y476]" using "Anti-SHP1 antibody [Y476] ab32559"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with Anti-SHP1 antibody [Y476] ab32559 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008), expandable thumbnail](https://content.abcam.com/products/images/shp1-antibody-y476-bsa-and-azide-free-ab226008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423105.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008)
Tissue Microarrays stained for "Anti-SHP1 antibody [Y476]" using "Anti-SHP1 antibody [Y476] ab32559"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with Anti-SHP1 antibody [Y476] ab32559 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008), expandable thumbnail](https://content.abcam.com/products/images/shp1-antibody-y476-bsa-and-azide-free-ab226008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423106.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008), expandable thumbnail](https://content.abcam.com/products/images/shp1-antibody-y476-bsa-and-azide-free-ab226008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423105.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] - BSA and Azide free (ab226008)")
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