Rabbit Polyclonal SHP1 phospho Y536 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PTPN6 phospho Y536 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Nature nanotechnology 15:154-1612020Pro-efferocytic nanoparticles are specifically taken up by lesional macrophages and prevent atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlyssa M Flores et. al.PubMed 31988506
- Cell death and differentiation 27:525-5392019Resolvin D1 promotes the targeting and clearance of necroptotic cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrennan D Gerlach,Michael Marinello,Justin Heinz,Nicholas Rymut,Brian E Sansbury,Colin O Riley,Sudeshna Sadhu,Zeinab Hosseini,Yoko Kojima,Dale D Tang,Nicholas J Leeper,Matthew Spite,Margarida Barroso,Katey J Rayner,Gabrielle FredmanPubMed 31222041
- Circulation 137:750-7532018Proefferocytic Therapy Promotes Transforming Growth Factor-β Signaling and Prevents Aneurysm Formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoko Kojima,Norna Werner,Jianqin Ye,Vivek Nanda,Noah Tsao,Ying Wang,Alyssa M Flores,Clint L Miller,Irving Weissman,Hongping Deng,Baohui Xu,Ronald L Dalman,Suzanne M Eken,Jaroslav Pelisek,Yuhuang Li,Lars Maegdefessel,Nicholas J LeeperPubMed 29440201
- Nature 536:86-902016CD47-blocking antibodies restore phagocytosis and prevent atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoko Kojima et. al.PubMed 27437576
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human PTPN6 phospho Y536 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P29350
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.
Alternative names
Tyrosine-protein phosphatase non-receptor type 6, Hematopoietic cell protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1, SH-PTP1, PTP-1C, PTPN6, PTP1C, HCP
Recommended products
Rabbit Polyclonal SHP1 phospho Y536 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PTPN6 phospho Y536 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human PTPN6 phospho Y536 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P29350
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab131500 was purified by affinity chromatography using epitope specific phosphopeptide. Non-phosphospecific antibodies were removed by chromatography using non-phosphopeptide.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SHP1 also known as PTPN6 belongs to the family of protein tyrosine phosphatases and has a protein mass of approximately 68 kDa. It plays a critical role in cell signaling by dephosphorylating specific targets leading to the modulation of signal transduction pathways. SHP1 is ubiquitously expressed with high levels observed in hematopoietic cells suggesting a specialized function in the immune system.
- Biological function summary
SHP1 influences cell proliferation survival and differentiation by acting as a negative regulator of signaling pathways. It is not a part of a larger protein complex but it associates transiently with different receptors and substrates. It plays an important role in the immune response by regulating cytokine signaling which is important for maintaining the balance between immune activation and tolerance.
- Pathways
SHP1 significantly impacts the JAK/STAT and B cell receptor signaling pathways. In the JAK/STAT pathway SHP1 interacts with JAK kinases contributing to the downregulation of cytokine signaling. In B cell receptor signaling it associates with Syk influencing immune cell activation and function.
- Associated diseases and disorders
SHP1 has a role in autoimmune diseases such as rheumatoid arthritis and certain types of leukemia. In rheumatoid arthritis defective regulation by SHP1 can lead to excessive inflammation while in leukemia its altered activity impacts cell growth and survival. It interacts with proteins such as STATs and SOCS which are important in these pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-SHP1 (phospho Y536) antibody (ab131500)
Immunofluorescent analysis of methanol-fixed HeLa cells labelling SHP1 (phospho Y536) with ab131500 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 (phospho Y536) antibody (ab131500)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling SHP1 (phospho Y536) with ab131500 at 1/50 dilution (left) or ab131500 preincubated with blocking peptide (right).
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