Rabbit Polyclonal SHP1 phospho Y536 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human PTPN6 phospho Y536 aa 500 to C-terminus.
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Publications
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- Frontiers in cell and developmental biology 9:6460772021WASp Is Crucial for the Unique Architecture of the Immunological Synapse in Germinal Center B-Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanan Li,Anshuman Bhanja,Arpita Upadhyaya,Xiaodong Zhao,Wenxia SongPubMed 34195186
- PLoS pathogens 16:e10091362020Runx proteins mediate protective immunity against Leishmania donovani infection by promoting CD40 expression on dendritic cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMd Naushad Akhtar et. al.PubMed 33370418
- International journal of clinical and experimental 11:5612-56212018CD47 is associated with the up-regulation of the PD-1 oncogenic signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanzhen Yao et. al.PubMed 31949648
- The Journal of experimental medicine 215:1337-13472018SHP-1 regulates hematopoietic stem cell quiescence by coordinating TGF-β signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinjia Jiang et. al.PubMed 29669741
- Nature communications 8:158692017Somatic mutations in clonally expanded cytotoxic T lymphocytes in patients with newly diagnosed rheumatoid arthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesP Savola et. al.PubMed 28635960
- Nature neuroscience 20:917-9262017PD-L1 inhibits acute and chronic pain by suppressing nociceptive neuron activity via PD-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGang Chen et. al.PubMed 28530662
- Nature immunology 17:1273-12812016The mucin MUC1 modulates the tumor immunological microenvironment through engagement of the lectin Siglec-9.
- Nature cell biology 17:665-772015The ITIM-containing receptor LAIR1 is essential for acute myeloid leukaemia development.
- The Journal of biological chemistry 288:16738-462013Discoidin domain receptor 1 protein is a novel modulator of megakaryocyte-collagen interactions.
- PloS one 6:e227762011Lymphocyte cell-cycle inhibition by HLA-G is mediated by phosphatase SHP-2 and acts on the mTOR pathway.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human PTPN6 phospho Y536 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P29350
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/300.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.
Alternative names
Tyrosine-protein phosphatase non-receptor type 6, Hematopoietic cell protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1, SH-PTP1, PTP-1C, PTPN6, PTP1C, HCP
Recommended products
Rabbit Polyclonal SHP1 phospho Y536 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human PTPN6 phospho Y536 aa 500 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human PTPN6 phospho Y536 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P29350
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab51171 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SHP1 also known as PTPN6 belongs to the family of protein tyrosine phosphatases and has a protein mass of approximately 68 kDa. It plays a critical role in cell signaling by dephosphorylating specific targets leading to the modulation of signal transduction pathways. SHP1 is ubiquitously expressed with high levels observed in hematopoietic cells suggesting a specialized function in the immune system.
- Biological function summary
SHP1 influences cell proliferation survival and differentiation by acting as a negative regulator of signaling pathways. It is not a part of a larger protein complex but it associates transiently with different receptors and substrates. It plays an important role in the immune response by regulating cytokine signaling which is important for maintaining the balance between immune activation and tolerance.
- Pathways
SHP1 significantly impacts the JAK/STAT and B cell receptor signaling pathways. In the JAK/STAT pathway SHP1 interacts with JAK kinases contributing to the downregulation of cytokine signaling. In B cell receptor signaling it associates with Syk influencing immune cell activation and function.
- Associated diseases and disorders
SHP1 has a role in autoimmune diseases such as rheumatoid arthritis and certain types of leukemia. In rheumatoid arthritis defective regulation by SHP1 can lead to excessive inflammation while in leukemia its altered activity impacts cell growth and survival. It interacts with proteins such as STATs and SOCS which are important in these pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 (phospho Y536) antibody (ab51171)
ab51171 at 1/50 dilution staining SHP1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
Western blot - Anti-SHP1 (phospho Y536) antibody (ab51171)
Western blot analysis of extracts from RAW264.7 cells treated with EGF (200ng/ml, 5 minutes) Western blot analysis of extracts from RAW264.7 cells treated with EGF (200ng/ml, 5 minutes)
All lanes: Western blot - Anti-SHP1 (phospho Y536) antibody (ab51171)
Predicted band size: 68 kDa
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