Anti-SHP2 antibody [79/PTP1D/SHP2] (BSA and Azide free)
- BOND RX™ Validated
- Recombinant
- KO Validated
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Mouse Recombinant Monoclonal SHP2 antibody. Carrier free. Suitable for IP, IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples.
View Alternative Names
PTP2C, SHPTP2, PTPN11, Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, SH-PTP2, SH-PTP3, PTP-1D, PTP-2C, SHP-2, Shp2
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP2 antibody [79/PTP1D/SHP2] (BSA and Azide free) (AB290657)
This data was developed using ab290646, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling SHP2 with ab290646 at 1/500 (2.018 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining in human liver (PMID : 8216283).The section was incubated with ab290646 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP2 antibody [79/PTP1D/SHP2] (BSA and Azide free) (AB290657)
This data was developed using ab290646, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labelling SHP2 with ab290646 at 1/500 (2.018 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining in human ovarian cancer.The section was incubated with ab290646 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-SHP2 antibody [79/PTP1D/SHP2] (BSA and Azide free) (AB290657)
Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized Jurkat (human T cell leukemia T lymphocyte) cells labeling SHP2 with ab290646 at 1/50 (20.18 µg/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing cytoplasmic staining in Jurkat cell line is observed. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 10 µg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 µg/ml dilution.
- IP
Supplier Data
Immunoprecipitation - Anti-SHP2 antibody [79/PTP1D/SHP2] (BSA and Azide free) (AB290657)
Lane 1:
Immunoprecipitation - Anti-SHP2 antibody [79/PTP1D/SHP2] (<a href='/en-us/products/primary-antibodies/shp2-antibody-79-ptp1d-shp2-ab290646'>ab290646</a>) at 1/30 dilution
Lane 2:
Immunoprecipitation - Anti-SHP2 antibody [79/PTP1D/SHP2] (<a href='/en-us/products/primary-antibodies/shp2-antibody-79-ptp1d-shp2-ab290646'>ab290646</a>) at 1/1000 dilution
All lanes:
HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
Predicted band size: 68 kDa
false
Exposure time: 3s
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP2 antibody [79/PTP1D/SHP2] (BSA and Azide free) (AB290657)
This data was developed using ab290646, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling SHP2 with ab290646 at 1/2000 (0.505 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining in rat cerebrum.The section was incubated with ab290646 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP2 antibody [79/PTP1D/SHP2] (BSA and Azide free) (AB290657)
This data was developed using ab290646, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling SHP2 with ab290646 at 1/2000 (0.505 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining in mouse kidney.The section was incubated with ab290646 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP2 antibody [79/PTP1D/SHP2] (BSA and Azide free) (AB290657)
This data was developed using ab290646, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling SHP2 with ab290646 at 1/2000 (0.505 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining in mouse cerebrum.The section was incubated with ab290646 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP2 antibody [79/PTP1D/SHP2] (BSA and Azide free) (AB290657)
This data was developed using ab290646, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling SHP2 with ab290646 at 1/2000 (0.505 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining in rat kidney.The section was incubated with ab290646 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IP
Supplier Data
Immunoprecipitation - Anti-SHP2 antibody [79/PTP1D/SHP2] (BSA and Azide free) (AB290657)
This data was developed using ab290646, the same antibody clone in a different buffer formulation. SHP2 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 µg with ab290646 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab290646 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 µg Lane 2 : ab290646 IP in NIH/3T3 whole cell lysate Lane 3 : Mouse IgG1 monoclonal (ab18443) instead of ab290646 in NIH/3T3 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 seconds
Lane 1:
Immunoprecipitation - Anti-SHP2 antibody [79/PTP1D/SHP2] (<a href='/en-us/products/primary-antibodies/shp2-antibody-79-ptp1d-shp2-ab290646'>ab290646</a>) at 1/30 dilution
Lane 2:
Immunoprecipitation - Anti-SHP2 antibody [79/PTP1D/SHP2] (<a href='/en-us/products/primary-antibodies/shp2-antibody-79-ptp1d-shp2-ab290646'>ab290646</a>) at 1/1000 dilution
All lanes:
NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg
Predicted band size: 68 kDa
false
Exposure time: 3s
- WB
Lab
Western blot - Anti-SHP2 antibody [79/PTP1D/SHP2] (BSA and Azide free) (AB290657)
This data was developed using 290646, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Lysates should be made freshly and used in WB immediately to minimize protein degradation.
All lanes:
Western blot - Anti-SHP2 antibody [79/PTP1D/SHP2] (<a href='/en-us/products/primary-antibodies/shp2-antibody-79-ptp1d-shp2-ab290646'>ab290646</a>)
Lane 1:
HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2:
293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 3:
NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Lane 4:
PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg
Lane 5:
Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg
Lane 6:
RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg
Secondary
All lanes:
Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
false
Exposure time: 10s
- WB
Lab
Western blot - Anti-SHP2 antibody [79/PTP1D/SHP2] (BSA and Azide free) (AB290657)
Blocking and diluting buffer and concentration : False colour image of Western blot : Anti-SHP2 antibody [79/PTP1D/SHP2] (ab290646 staining at 1/1000 dilution, shown in green; Rabbit anti-GAPDH antibody [16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab290646 was shown to bind specifically to SHP2(PTPN11). A band was observed at 68 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in the PTPN11 knockout cell line. To generate this image, wild-type and PTPN11 knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a PVDF-FL membrane. Membranes were blocked in Odyssey diluted in equal volume of 0.1 % TBS before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Mouse IgG H&L (IRDye® 800CW) (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (ab216777) at 1/10000 dilution. Performed under reducing conditions.
All lanes:
Western blot - Anti-SHP2 antibody [79/PTP1D/SHP2] (<a href='/en-us/products/primary-antibodies/shp2-antibody-79-ptp1d-shp2-ab290646'>ab290646</a>) at 1/1000 dilution
Lane 1:
Wild type HEK-293T (human embryonic kidney epithelial cell) at 20 µg
Lane 2:
PTPN11 knockout cell line at 20 µg
Secondary
Lanes 1 - 2:
Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/100000 dilution
Lanes 1 - 2:
Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) at 1/10000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
false
- WB
Lab
Western blot - Anti-SHP2 antibody [79/PTP1D/SHP2] (BSA and Azide free) (AB290657)
This data was developed using 290646, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Exposure time : Lanes 1/2 : 37 seconds Lanes 3/4 : 8 seconds
All lanes:
Western blot - Anti-SHP2 antibody [79/PTP1D/SHP2] (<a href='/en-us/products/primary-antibodies/shp2-antibody-79-ptp1d-shp2-ab290646'>ab290646</a>) at 1/1000 dilution
Lane 1:
Mouse heart tissue lysate at 10 µg
Lane 2:
Mouse kidney tissue lysate at 10 µg
Lane 3:
Rat brain tissue lysate at 10 µg
Lane 4:
Rat heart tissue lysate at 10 µg
Secondary
All lanes:
Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
false
Reactivity data
Product details
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The role of SHP2 extends to involvement in several signaling cascades such as the Ras/MAPK and PI3K/AKT pathways. It functions as an essential component within protein complexes that facilitate cell communication and response to external signals. The protein modulates growth factor signaling and cytokine signaling highlighting its significance in normal cell function and development. SHP2's statement in signaling processes makes it an important regulator of cellular dynamics.
Pathways
SHP2 participates in the Ras/MAPK and PI3K/AKT signaling pathways which are important for regulating cell growth survival and differentiation. Within these pathways SHP2 interacts with various signaling molecules including Grb2 Sos and Gab family adaptors. These interactions coordinate cellular responses to growth factors and other extracellular cues ensuring proper pathway activation and control. By serving as a critical mediator SHP2 integrates signals that are necessary for appropriate cellular outcomes.
Product protocols
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Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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