Anti-SHP2 antibody

• WB

Lab

Western blot - Anti-SHP2 antibody (AB10555)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : SHP2 knockout HAP1 cell lysate (20 μg)
Lane 3 : A431 cell lysate (20 μg)
Lane 4 : Jurkat cell lysate (20 μg)
Lanes 1 to 4 : Merged signal (red and green). Green - ab10555 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab10555 was shown to recognize SHP2 when SHP2 knockout samples were used, along with additional cross-reactive bands. Wild-type and SHP2 knockout samples were subjected to SDS-PAGE. ab10555 and ab8245 (loading control to GAPDH) were both diluted at 1 μg/mL and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-SHP2 antibody (ab10555)

Predicted band size: 68 kDa

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• WB

Unknown

Western blot - Anti-SHP2 antibody (AB10555)

Jurkat cell lysates (lanes 1 and 2) were probed with ab10555 at 1/500. This antibody recognises SHP2 at 68 kDa. The lower molecular weight band may represent a variant or differences in the post-translational modification of the protein.
M : marker
lane 1 : ab10555
lane 2 : ab10555 + blocking peptide

M=Marker

All lanes:

Western blot - Anti-SHP2 antibody (ab10555) at 2 µg/mL

Lane 1:

Jurkat lysate at 20 µg

Lane 2:

Jurkat at 20 µg with Human SHP2 peptide (<a href='/en-us/products/unavailable/human-shp2-peptide-ab14800'>ab14800</a>)

Predicted band size: 68 kDa

Observed band size: 68 kDa

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