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Rabbit Recombinant Monoclonal SHP2 antibody. Suitable for IP, WB and reacts with Mouse, Human, Rat samples. Cited in 3 publications.

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Images

Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (AB187040), expandable thumbnail
  • Western blot - Anti-SHP2 antibody [EPR17829-9] (AB187040), expandable thumbnail
  • Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (AB187040), expandable thumbnail
  • Western blot - Anti-SHP2 antibody [EPR17829-9] (AB187040), expandable thumbnail
  • Western blot - Anti-SHP2 antibody [EPR17829-9] (AB187040), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Tested
Tested
Rat
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/40
Notes

-

Species
Human
Dilution info
1/40
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

-

Species
Rat
Dilution info
1/5000
Notes

-

Species
Human
Dilution info
1/5000
Notes

-

Associated Products

Select an associated product type

13 products for Alternative Product

Target data

Function

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed:10655584, PubMed:14739280, PubMed:18559669, PubMed:18829466, PubMed:26742426, PubMed:28074573). Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (PubMed:18559669). Dephosphorylates CDC73 (PubMed:26742426). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity). Dephosphorylates tyrosine-phosphorylated NEDD9/CAS-L (PubMed:19275884).

Alternative names

PTP2C, SHPTP2, PTPN11, Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, SH-PTP2, SH-PTP3, PTP-1D, PTP-2C, SHP-2, Shp2

Recommended products

Rabbit Recombinant Monoclonal SHP2 antibody. Suitable for IP, WB and reacts with Mouse, Human, Rat samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17829-9
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SHP2 also known as PTPN11 is a protein tyrosine phosphatase with a molecular mass of approximately 68 kDa. It is expressed in various tissues including the heart liver and immune cells. SHP2 belongs to the non-receptor class of protein tyrosine phosphatases and plays a critical role in cell signaling by acting as a regulator of signal transduction processes. SHP2 mediates these processes by dephosphorylating specific phosphotyrosine residues on target proteins influencing various cellular functions like proliferation differentiation and survival.

Biological function summary

The role of SHP2 extends to involvement in several signaling cascades such as the Ras/MAPK and PI3K/AKT pathways. It functions as an essential component within protein complexes that facilitate cell communication and response to external signals. The protein modulates growth factor signaling and cytokine signaling highlighting its significance in normal cell function and development. SHP2's statement in signaling processes makes it an important regulator of cellular dynamics.

Pathways

SHP2 participates in the Ras/MAPK and PI3K/AKT signaling pathways which are important for regulating cell growth survival and differentiation. Within these pathways SHP2 interacts with various signaling molecules including Grb2 Sos and Gab family adaptors. These interactions coordinate cellular responses to growth factors and other extracellular cues ensuring proper pathway activation and control. By serving as a critical mediator SHP2 integrates signals that are necessary for appropriate cellular outcomes.

Associated diseases and disorders

SHP2 is associated with several conditions such as Noonan syndrome and various cancers. Mutations in the PTPN11 gene which encodes SHP2 often result in aberrant signaling that leads to developmental anomalies or tumorigenesis. In Noonan syndrome the mutated SHP2 protein results in disrupted Ras/MAPK pathway signaling. As for cancers SHP2 is often found to be overactive leading to enhanced cell proliferation and survival. In these contexts SHP2 is interconnected with other proteins like RAS and RAF which also contribute to oncogenic pathway activation and disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (ab187040), expandable thumbnail

    Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (ab187040)

    SHP2 was immunoprecipitated from 1 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab187040 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab187040 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (Input).

    Lane 2: ab187040 IP in HeLa whole cell lysate (+).

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab187040 in HeLa whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (ab187040)

    Developed using the ECL technique.

    Predicted band size: 43 kDa, 68 kDa

    Observed band size: 68 kDa

  • Western blot - Anti-SHP2 antibody [EPR17829-9] (ab187040), expandable thumbnail

    Western blot - Anti-SHP2 antibody [EPR17829-9] (ab187040)

    Lanes 1- 2: Merged signal (red and green). Green - ab187040 observed at 68 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab187040 was shown to react with SHP2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line Human PTPN11 (SHP2) knockout HEK-293T cell line ab266450 (knockout cell lysate Human PTPN11 (SHP2) knockout HEK-293T cell lysate ab257618) was used. Wild-type HEK-293T and PTPN11 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab187040 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SHP2 antibody [EPR17829-9] (ab187040) at 1/5000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: PTPN11 knockout HEK-293T cell lysate at 20 µg

    Lane 2: Western blot - Human PTPN11 (SHP2) knockout HEK-293T cell line (Human PTPN11 (SHP2) knockout HEK-293T cell line ab266450)

    Performed under reducing conditions.

    Predicted band size: 68 kDa

    Observed band size: 68 kDa

  • Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (ab187040), expandable thumbnail

    Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (ab187040)

    SHP2 was immunoprecipitated from 1 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab187040 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab187040 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10μg (Input).

    Lane 2: ab187040 IP in NIH/3T3 whole cell lysate (+).

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab187040 in NIH/3T3 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    All lanes: Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (ab187040)

    Developed using the ECL technique.

    Predicted band size: 68 kDa

    Observed band size: 68 kDa

  • Western blot - Anti-SHP2 antibody [EPR17829-9] (ab187040), expandable thumbnail

    Western blot - Anti-SHP2 antibody [EPR17829-9] (ab187040)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SHP2 antibody [EPR17829-9] (ab187040) at 1/5000 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

    Lane 2: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 5 µg

    Lane 3: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 5 µg

    Lane 4: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 5 µg

    Lane 5: Mouse brain tissue lysate at 5 µg

    Lane 6: Mouse heart tissue lysate at 5 µg

    Lane 7: Rat brain tissue lysate at 5 µg

    Lane 8: Rat heart tissue lysate at 5 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa

    Observed band size: 68 kDa

    Exposure time: 1min

  • Western blot - Anti-SHP2 antibody [EPR17829-9] (ab187040), expandable thumbnail

    Western blot - Anti-SHP2 antibody [EPR17829-9] (ab187040)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time Lane 1-8: 180s

    Exposure time Lane 9-10: 1s

    Exposure time Lane 11-16: 15s

    Anti-SHP2 antibody [EPR26539-45] ab300579 is less sensitive than ab187040 in WB. We suggest customers choosing ab187040 for WB test.

    Lanes 1 - 8: Western blot - Anti-SHP2 antibody [EPR26539-45] (Anti-SHP2 antibody [EPR26539-45] ab300579) at 1/1000 dilution

    Lanes 9 - 16: Western blot - Anti-SHP2 antibody [EPR17829-9] (ab187040) at 1/1000 dilution

    Lanes 1 and 9: Human cerebellum tissue lysate, 20 μg

    Lanes 2 and 10: Human heart tissue lysate, 20 μg

    Lanes 3 and 11: Mouse ovary tissue lysate, 20 μg

    Lanes 4 and 12: Mouse embryo tissue lysate, 20 μg

    Lanes 5 and 13: Mouse brain tissue lysate, 20 μg

    Lanes 6 and 14: Mouse heart tissue lysate, 20 μg

    Lanes 7 and 14: Rat brain tissue lysate, 20 μg

    Lanes 8 and 16: Rat heart tissue lysate, 20 μg

    Secondary

    Lanes 1, 2, 9 and 10: Goat Anti-Rabbit IgG (HRP), minimal cross-reactivity with human IgG at 1/2000 dilution

    Lanes 3, 4, 5, 6, 7, 8, 11, 12, 13, 14, 15 and 16: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 68 kDa

    Observed band size: 72 kDa

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