Skip to main content

Rabbit Recombinant Monoclonal SHP2 antibody. Suitable for IP, WB and reacts with Mouse, Human, Rat samples. Cited in 3 publications.

Be the first to review this product! Submit a review

Images

Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (AB187040), expandable thumbnail
  • Western blot - Anti-SHP2 antibody [EPR17829-9] (AB187040), expandable thumbnail
  • Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (AB187040), expandable thumbnail
  • Western blot - Anti-SHP2 antibody [EPR17829-9] (AB187040), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Tested
Tested
Rat
Expected
Tested

Tested
Tested

Species

Mouse

Dilution info

1/40

Notes

-

Species

Human

Dilution info

1/40

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/5000

Notes

-

Species

Rat

Dilution info

1/5000

Notes

-

Species

Human

Dilution info

1/5000

Notes

-

Associated Products

Select an associated product type

14 products for Alternative Product

Target data

Function

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed:10655584, PubMed:18559669, PubMed:18829466, PubMed:26742426, PubMed:28074573). Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (PubMed:18559669). Dephosphorylates CDC73 (PubMed:26742426). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity).

Alternative names

Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, SH-PTP2, SH-PTP3, PTP-1D, PTP-2C, SHP-2, Shp2, PTPN11, PTP2C, SHPTP2

Recommended products

Rabbit Recombinant Monoclonal SHP2 antibody. Suitable for IP, WB and reacts with Mouse, Human, Rat samples. Cited in 3 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR17829-9

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (ab187040), expandable thumbnail

    Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (ab187040)

    SHP2 was immunoprecipitated from 1 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab187040 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab187040 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (Input).

    Lane 2: ab187040 IP in HeLa whole cell lysate (+).

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab187040 in HeLa whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (ab187040)

    Developed using the ECL technique.

    Predicted band size: 43 kDa, 68 kDa

    Observed band size: 68 kDa

  • Western blot - Anti-SHP2 antibody [EPR17829-9] (ab187040), expandable thumbnail

    Western blot - Anti-SHP2 antibody [EPR17829-9] (ab187040)

    Lanes 1- 2: Merged signal (red and green). Green - ab187040 observed at 68 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab187040 was shown to react with SHP2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line Human PTPN11 (SHP2) knockout HEK-293T cell line ab266450 (knockout cell lysate Human PTPN11 (SHP2) knockout HEK-293T cell lysate ab257618) was used. Wild-type HEK-293T and PTPN11 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab187040 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SHP2 antibody [EPR17829-9] (ab187040) at 1/5000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: PTPN11 knockout HEK-293T cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 68 kDa

    Observed band size: 68 kDa

  • Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (ab187040), expandable thumbnail

    Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (ab187040)

    SHP2 was immunoprecipitated from 1 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab187040 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab187040 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10μg (Input).

    Lane 2: ab187040 IP in NIH/3T3 whole cell lysate (+).

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab187040 in NIH/3T3 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    All lanes: Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (ab187040)

    Developed using the ECL technique.

    Predicted band size: 68 kDa

    Observed band size: 68 kDa

  • Western blot - Anti-SHP2 antibody [EPR17829-9] (ab187040), expandable thumbnail

    Western blot - Anti-SHP2 antibody [EPR17829-9] (ab187040)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SHP2 antibody [EPR17829-9] (ab187040) at 1/5000 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

    Lane 2: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 5 µg

    Lane 3: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 5 µg

    Lane 4: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 5 µg

    Lane 5: Mouse brain tissue lysate at 5 µg

    Lane 6: Mouse heart tissue lysate at 5 µg

    Lane 7: Rat brain tissue lysate at 5 µg

    Lane 8: Rat heart tissue lysate at 5 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa

    Observed band size: 68 kDa

    Exposure time: 1min

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com