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AB76285

Anti-SHP2 antibody [M163]

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(4 Publications)

Mouse Monoclonal SHP2 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PTPN11.

View Alternative Names

PTP2C, SHPTP2, PTPN11, Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, SH-PTP2, SH-PTP3, PTP-1D, PTP-2C, SHP-2, Shp2

2 Images
Western blot - Anti-SHP2 antibody [M163] (AB76285)
  • WB

Lab

Western blot - Anti-SHP2 antibody [M163] (AB76285)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : SHP2 knockout HAP1 cell lysate (20 μg)
Lane 3 : A431 cell lysate (20 μg)
Lane 4 : Jurkat cell lysate (20 μg)
Lanes 1 to 4 : Merged signal (red and green). Green - ab76285 observed at 68 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76285 was shown to specifically react with SHP2 when SHP2 knockout samples were used. Wild-type and SHP2 knockout samples were subjected to SDS-PAGE. ab76285 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-SHP2 antibody [M163] (ab76285)

Predicted band size: 68 kDa

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Western blot - Anti-SHP2 antibody [M163] (AB76285)
  • WB

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Western blot - Anti-SHP2 antibody [M163] (AB76285)

Lane 1:

Western blot - Anti-SHP2 antibody [M163] (ab76285) at 1/250 dilution

Lane 2:

Western blot - Anti-SHP2 antibody [M163] (ab76285) at 1/500 dilution

Lane 3:

Western blot - Anti-SHP2 antibody [M163] (ab76285) at 1/1000 dilution

Lane 4:

Western blot - Anti-SHP2 antibody [M163] (ab76285) at 1/2000 dilution

All lanes:

adult mouse brain lysates

Predicted band size: 68 kDa

Observed band size: 72 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

M163

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human PTPN11. The exact immunogen used to generate this antibody is proprietary information.

Q06124

Specificity

ab76285 does not cross-react with SHP1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHP2 also known as PTPN11 is a protein tyrosine phosphatase with a molecular mass of approximately 68 kDa. It is expressed in various tissues including the heart liver and immune cells. SHP2 belongs to the non-receptor class of protein tyrosine phosphatases and plays a critical role in cell signaling by acting as a regulator of signal transduction processes. SHP2 mediates these processes by dephosphorylating specific phosphotyrosine residues on target proteins influencing various cellular functions like proliferation differentiation and survival.
Biological function summary

The role of SHP2 extends to involvement in several signaling cascades such as the Ras/MAPK and PI3K/AKT pathways. It functions as an essential component within protein complexes that facilitate cell communication and response to external signals. The protein modulates growth factor signaling and cytokine signaling highlighting its significance in normal cell function and development. SHP2's statement in signaling processes makes it an important regulator of cellular dynamics.

Pathways

SHP2 participates in the Ras/MAPK and PI3K/AKT signaling pathways which are important for regulating cell growth survival and differentiation. Within these pathways SHP2 interacts with various signaling molecules including Grb2 Sos and Gab family adaptors. These interactions coordinate cellular responses to growth factors and other extracellular cues ensuring proper pathway activation and control. By serving as a critical mediator SHP2 integrates signals that are necessary for appropriate cellular outcomes.

SHP2 is associated with several conditions such as Noonan syndrome and various cancers. Mutations in the PTPN11 gene which encodes SHP2 often result in aberrant signaling that leads to developmental anomalies or tumorigenesis. In Noonan syndrome the mutated SHP2 protein results in disrupted Ras/MAPK pathway signaling. As for cancers SHP2 is often found to be overactive leading to enhanced cell proliferation and survival. In these contexts SHP2 is interconnected with other proteins like RAS and RAF which also contribute to oncogenic pathway activation and disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed : 10655584, PubMed : 14739280, PubMed : 18559669, PubMed : 18829466, PubMed : 26742426, PubMed : 28074573, PubMed : 32184441). Positively regulates MAPK signal transduction pathway (PubMed : 28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed : 28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (PubMed : 18559669). Dephosphorylates CDC73 (PubMed : 26742426). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity). Dephosphorylates tyrosine-phosphorylated NEDD9/CAS-L (PubMed : 19275884). Acts as an effector of PDCD1-mediated inhibition of T-cell response : recruited by phosphorylated PDCD1, mediating dephosphorylation of key T-cell receptor (TCR) proximal signaling molecules, leading to TCR signaling inhibition (PubMed : 32184441).
See full target information PTPN11
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 19:4579-4588 PubMed30942400

2019

YB1 protects cardiac myocytes against H2O2‑induced injury via suppression of PIAS3 mRNA and phosphorylation of STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Shiqi Wang,Fuwei He,Zhenwei Li,Yewen Hu,Ning Huangfu,Xiaomin Chen

Science signaling 11: PubMed30459283

2018

Integrated proximal proteomics reveals IRS2 as a determinant of cell survival in ALK-driven neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina B Emdal,Anna-Kathrine Pedersen,Dorte B Bekker-Jensen,Alicia Lundby,Shana Claeys,Katleen De Preter,Frank Speleman,Chiara Francavilla,Jesper V Olsen

PLoS genetics 10:e1004364 PubMed24875294

2014

SHP2 regulates chondrocyte terminal differentiation, growth plate architecture and skeletal cell fates.

Applications

WB

Species

Mouse

Margot E Bowen,Ugur M Ayturk,Kyle C Kurek,Wentian Yang,Matthew L Warman

British journal of cancer 103:877-84 PubMed20700123

2010

miR-489 is a tumour-suppressive miRNA target PTPN11 in hypopharyngeal squamous cell carcinoma (HSCC).

Applications

WB

Species

Human

N Kikkawa,T Hanazawa,L Fujimura,N Nohata,H Suzuki,H Chazono,D Sakurai,S Horiguchi,Y Okamoto,N Seki
View all publications
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