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AB225574

Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free

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(4 Publications)

Rabbit Recombinant Monoclonal SHP2 phospho Y542 antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications.

View Alternative Names

PTP2C, SHPTP2, PTPN11, Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, SH-PTP2, SH-PTP3, PTP-1D, PTP-2C, SHP-2, Shp2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (AB225574)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (AB225574)

This ICC/IF data was generated using the same anti-SHP2 (phospho Y542) antibody clone [EP608(2)Y] in a different buffer formulation (cat# ab62322).

ab62322 staining SHP2 in THP-1 cells ( Human monocytic leukaemia cell line) by ICC/IF (immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with purified ab62322 at 8.8 μg/ml. Secondary antibody used was AlexaFluor®488 Goat anti-Rabbit (ab150077) at 2 μg/ml. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)( ab195889) used as counterstain at 2.5 μg/ml . DAPI was used as nuclear counterstain. Confocal image showing the expression was increased after treatment with TPA 200nM for 24h and PDGF 50ng/ml for 30min, the signal decreased after treatment with Lambda Protein Phosphatase 31℃ for 2h.

This image was generated using the unpurified version of the product.

Immunoprecipitation - Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (AB225574)
  • IP

Unknown

Immunoprecipitation - Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (AB225574)

This ICC/IF data was generated using the same anti-SHP2 (phospho Y542) antibody clone [EP608(2)Y] in a different buffer formulation (cat# ab62322).

ab62322 (purified) at 1 : 30 dilution (2μg) immunoprecipitating SHP2 in NIH/3T3 treated with 50ng/ml PDGF for 40min whole cell lysate.
Lane 1 (input) : NIH/3T3 (Mouse embryonic fibroblast) treated with 50ng/ml PDGF for 40min whole cell lysate 10μg
Lane 2 (+) : ab62322 & NIH/3T3 treated with 50ng/ml PDGF for 40min whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab62322 in NIH/3T3 treated with 50ng/ml PDGF for 40min whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] (<a href='/en-us/products/primary-antibodies/shp2-phospho-y542-antibody-ep5082y-ab62322'>ab62322</a>)

Predicted band size: 68 kDa

false

  • Unconjugated

    Anti-SHP2 (phospho Y542) antibody [EP508(2)Y]

  • 578 PE

    PE Anti-SHP2 (phospho Y542) antibody [EP508(2)Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-SHP2 (phospho Y542) antibody [EP508(2)Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-SHP2 (phospho Y542) antibody [EP508(2)Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-SHP2 (phospho Y542) antibody [EP508(2)Y]

  • 660 APC

    APC Anti-SHP2 (phospho Y542) antibody [EP508(2)Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-SHP2 (phospho Y542) antibody [EP508(2)Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP508(2)Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab225574 is the carrier-free version of ab62322.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHP2 also known as PTPN11 is a protein tyrosine phosphatase with a molecular mass of approximately 68 kDa. It is expressed in various tissues including the heart liver and immune cells. SHP2 belongs to the non-receptor class of protein tyrosine phosphatases and plays a critical role in cell signaling by acting as a regulator of signal transduction processes. SHP2 mediates these processes by dephosphorylating specific phosphotyrosine residues on target proteins influencing various cellular functions like proliferation differentiation and survival.
Biological function summary

The role of SHP2 extends to involvement in several signaling cascades such as the Ras/MAPK and PI3K/AKT pathways. It functions as an essential component within protein complexes that facilitate cell communication and response to external signals. The protein modulates growth factor signaling and cytokine signaling highlighting its significance in normal cell function and development. SHP2's statement in signaling processes makes it an important regulator of cellular dynamics.

Pathways

SHP2 participates in the Ras/MAPK and PI3K/AKT signaling pathways which are important for regulating cell growth survival and differentiation. Within these pathways SHP2 interacts with various signaling molecules including Grb2 Sos and Gab family adaptors. These interactions coordinate cellular responses to growth factors and other extracellular cues ensuring proper pathway activation and control. By serving as a critical mediator SHP2 integrates signals that are necessary for appropriate cellular outcomes.

SHP2 is associated with several conditions such as Noonan syndrome and various cancers. Mutations in the PTPN11 gene which encodes SHP2 often result in aberrant signaling that leads to developmental anomalies or tumorigenesis. In Noonan syndrome the mutated SHP2 protein results in disrupted Ras/MAPK pathway signaling. As for cancers SHP2 is often found to be overactive leading to enhanced cell proliferation and survival. In these contexts SHP2 is interconnected with other proteins like RAS and RAF which also contribute to oncogenic pathway activation and disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed : 10655584, PubMed : 14739280, PubMed : 18559669, PubMed : 18829466, PubMed : 26742426, PubMed : 28074573). Positively regulates MAPK signal transduction pathway (PubMed : 28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed : 28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (PubMed : 18559669). Dephosphorylates CDC73 (PubMed : 26742426). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity). Dephosphorylates tyrosine-phosphorylated NEDD9/CAS-L (PubMed : 19275884).
See full target information PTPN11 phospho Y542
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 42:1217-1238.e19 PubMed38981438

2024

Multi-scale signaling and tumor evolution in high-grade gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Jingxian Liu,Song Cao,Kathleen J Imbach,Marina A Gritsenko,Tung-Shing M Lih,Jennifer E Kyle,Tomer M Yaron-Barir,Zev A Binder,Yize Li,Ilya Strunilin,Yi-Ting Wang,Chia-Feng Tsai,Weiping Ma,Lijun Chen,Natalie M Clark,Andrew Shinkle,Nataly Naser Al Deen,Wagma Caravan,Andrew Houston,Faria Anjum Simin,Matthew A Wyczalkowski,Liang-Bo Wang,Erik Storrs,Siqi Chen,Ritvik Illindala,Yuping D Li,Reyka G Jayasinghe,Dmitry Rykunov,Sandra L Cottingham,Rosalie K Chu,Karl K Weitz,Ronald J Moore,Tyler Sagendorf,Vladislav A Petyuk,Michael Nestor,Lisa M Bramer,Kelly G Stratton,Athena A Schepmoes,Sneha P Couvillion,Josie Eder,Young-Mo Kim,Yuqian Gao,Thomas L Fillmore,Rui Zhao,Matthew E Monroe,Austin N Southard-Smith,Yang E Li,Rita Jui-Hsien Lu,Jared L Johnson,Maciej Wiznerowicz,Galen Hostetter,Chelsea J Newton,Karen A Ketchum,Ratna R Thangudu,Jill S Barnholtz-Sloan,Pei Wang,David Fenyö,Eunkyung An,Mathangi Thiagarajan,Ana I Robles,D R Mani,Richard D Smith,Eduard Porta-Pardo,Lewis C Cantley,Antonio Iavarone,Feng Chen,Mehdi Mesri,MacLean P Nasrallah,Hui Zhang,Adam C Resnick,Milan G Chheda,Karin D Rodland,Tao Liu,Li Ding

Oncogene 32:2346-55, 2355.e1-10 PubMed22777356

2012

Diminished functional role and altered localization of SHP2 in non-small cell lung cancer cells with EGFR-activating mutations.

Applications

Unspecified application

Species

Unspecified reactive species

C M Furcht,A R Muñoz Rojas,D Nihalani,M J Lazzara

Oncogene 29:4130-44 PubMed20473329

2010

SIRPalpha1 receptors interfere with the EGFRvIII signalosome to inhibit glioblastoma cell transformation and migration.

Applications

Unspecified application

Species

Human

G S Kapoor,D M O'Rourke

Experimental cell research 315:2343-57 PubMed19427850

2009

The protein tyrosine phosphatase SHP-2 is required for EGFRvIII oncogenic transformation in human glioblastoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhan,George J Counelis,Donald M O'Rourke
View all publications
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