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AB62379

Anti-SHP2 (phospho Y582) antibody [EP509Y]

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(1 Publication)

Rabbit Monoclonal SHP2 phospho Y582 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
1 Images
Western blot - Anti-SHP2 (phospho Y582) antibody [EP509Y] (AB62379)
  • WB

Unknown

Western blot - Anti-SHP2 (phospho Y582) antibody [EP509Y] (AB62379)

The bottom image shows beta Tubulin. Beta Tubulin has been included as a loading control

All lanes:

Western blot - Anti-SHP2 (phospho Y582) antibody [EP509Y] (ab62379) at 1/50000 dilution

Lane 1:

Jurkat cell lysate (untreated) at 10 µg

Lane 2:

Jurkat cell lysate (treated with pervanadate) at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP conjugate at 1/2000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa

false

  • Carrier free

    Anti-SHP2 (phospho Y582) antibody [EP509Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP509Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/25000 - 1/50000", "WB-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHP2 also known as PTPN11 is a protein tyrosine phosphatase with a molecular mass of approximately 68 kDa. It is expressed in various tissues including the heart liver and immune cells. SHP2 belongs to the non-receptor class of protein tyrosine phosphatases and plays a critical role in cell signaling by acting as a regulator of signal transduction processes. SHP2 mediates these processes by dephosphorylating specific phosphotyrosine residues on target proteins influencing various cellular functions like proliferation differentiation and survival.
Biological function summary

The role of SHP2 extends to involvement in several signaling cascades such as the Ras/MAPK and PI3K/AKT pathways. It functions as an essential component within protein complexes that facilitate cell communication and response to external signals. The protein modulates growth factor signaling and cytokine signaling highlighting its significance in normal cell function and development. SHP2's statement in signaling processes makes it an important regulator of cellular dynamics.

Pathways

SHP2 participates in the Ras/MAPK and PI3K/AKT signaling pathways which are important for regulating cell growth survival and differentiation. Within these pathways SHP2 interacts with various signaling molecules including Grb2 Sos and Gab family adaptors. These interactions coordinate cellular responses to growth factors and other extracellular cues ensuring proper pathway activation and control. By serving as a critical mediator SHP2 integrates signals that are necessary for appropriate cellular outcomes.

SHP2 is associated with several conditions such as Noonan syndrome and various cancers. Mutations in the PTPN11 gene which encodes SHP2 often result in aberrant signaling that leads to developmental anomalies or tumorigenesis. In Noonan syndrome the mutated SHP2 protein results in disrupted Ras/MAPK pathway signaling. As for cancers SHP2 is often found to be overactive leading to enhanced cell proliferation and survival. In these contexts SHP2 is interconnected with other proteins like RAS and RAF which also contribute to oncogenic pathway activation and disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed : 10655584, PubMed : 14739280, PubMed : 18559669, PubMed : 18829466, PubMed : 26742426, PubMed : 28074573, PubMed : 32184441). Positively regulates MAPK signal transduction pathway (PubMed : 28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed : 28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (PubMed : 18559669). Dephosphorylates CDC73 (PubMed : 26742426). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity). Dephosphorylates tyrosine-phosphorylated NEDD9/CAS-L (PubMed : 19275884). Acts as an effector of PDCD1-mediated inhibition of T-cell response : recruited by phosphorylated PDCD1, mediating dephosphorylation of key T-cell receptor (TCR) proximal signaling molecules, leading to TCR signaling inhibition (PubMed : 32184441).
See full target information PTPN11 pY582

Alternative Names

PTP2C, SHPTP2, PTPN11, Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, SH-PTP2, SH-PTP3, PTP-1D, PTP-2C, SHP-2, Shp2
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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

PloS one 5:e11584 PubMed20644732

2010

LRP-1 promotes cancer cell invasion by supporting ERK and inhibiting JNK signaling pathways.

Applications

WB

Species

Unspecified reactive species

Benoit Langlois,Gwenn Perrot,Christophe Schneider,Patrick Henriet,Hervé Emonard,Laurent Martiny,Stéphane Dedieu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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