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AB121316

Anti-SHROOM4 antibody

2

(1 Review)

|

(1 Publication)

Rabbit Polyclonal SHROOM4 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SHROOM4 aa 1150-1300.

View Alternative Names

KIAA1202, SHAP, SHROOM4, Protein Shroom4, Second homolog of apical protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHROOM4 antibody (AB121316)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHROOM4 antibody (AB121316)

ab121316, at 1/50 dilution, staining SHROOM4 in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-SHROOM4 antibody (AB121316)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SHROOM4 antibody (AB121316)

ab1216316, at 4μg/ml, staining SHROOM4 in in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SHROOM4 aa 1150-1300. The exact immunogen used to generate this antibody is proprietary information.

Q9ULL8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Store undiluted
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHROOM4 also known as KIAA1202 is a microtubule-associated protein with a mass of approximately 160 kDa. It plays a role in cytoskeletal organization by interacting with various cytoskeletal components. This protein is expressed mainly in neural tissues suggesting its involvement in neural development and function. SHROOM4's function in the cytoskeleton involves influencing the assembly and stability of actin filaments which are critical in maintaining the shape and motility of the cells.
Biological function summary

SHROOM4 participates in the regulation of cell morphogenesis particularly in the development of polarized cellular structures. SHROOM4 often associates with the actomyosin complex thereby influencing cell shape and polarity. By participating in these cellular processes SHROOM4 affects the organization and positioning of cells within tissues contributing to the proper formation of organ structures during development. Its interactions with other members of the SHROOM protein family enable it to execute its functions within the cellular architecture effectively.

Pathways

SHROOM4 is involved in the planar cell polarity pathway which is essential for coordinated orientation and arrangement of cells in the epithelial layer. This pathway also includes other key proteins like VANGL2. Besides SHROOM4 contributes to the actin cytoskeleton organization pathway where it engages with proteins like RHOA to modify actin dynamics. These interactions ensure precise cellular alignment and positioning critical for tissue and organ architecture during embryonic development.

SHROOM4 has connections to neurodevelopmental disorders such as epilepsy. Mutations in SHROOM4 correlate with disruption in neural circuit formation contributing to epileptic manifestations. Additionally SHROOM4's role in cytoarchitecture links it with conditions like X-linked intellectual disability. Proteins like ARHGEF6 accompany SHROOM4 in the disease mechanisms highlighting its involvement in maintaining cellular integrity and function in neural cells. Study of these associations reveals SHROOM4's significance in neurological health and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable regulator of cytoskeletal architecture that plays an important role in development. May regulate cellular and cytoskeletal architecture by modulating the spatial distribution of myosin II (By similarity).
See full target information SHROOM4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 8:95135-95151 PubMed29221117

2017

Inhibition of chemerin/CMKLR1 axis in neuroblastoma cells reduces clonogenicity and cell viability and impairs tumor growth .

Applications

Unspecified application

Species

Unspecified reactive species

Conny Tümmler,Igor Snapkov,Malin Wickström,Ugo Moens,Linda Ljungblad,Lotta Helena Maria Elfman,Jan-Olof Winberg,Per Kogner,John Inge Johnsen,Baldur Sveinbjørnsson
View all publications
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Product promise

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