Rabbit Polyclonal SHROOM4 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SHROOM4 aa 1150-1300.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SHROOM4 aa 1150-1300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9ULL8
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
Probable regulator of cytoskeletal architecture that plays an important role in development. May regulate cellular and cytoskeletal architecture by modulating the spatial distribution of myosin II (By similarity).
Alternative names
KIAA1202, SHAP, SHROOM4, Protein Shroom4, Second homolog of apical protein
Recommended products
Rabbit Polyclonal SHROOM4 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SHROOM4 aa 1150-1300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SHROOM4 aa 1150-1300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9ULL8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle, Store undiluted
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SHROOM4 also known as KIAA1202 is a microtubule-associated protein with a mass of approximately 160 kDa. It plays a role in cytoskeletal organization by interacting with various cytoskeletal components. This protein is expressed mainly in neural tissues suggesting its involvement in neural development and function. SHROOM4's function in the cytoskeleton involves influencing the assembly and stability of actin filaments which are critical in maintaining the shape and motility of the cells.
- Biological function summary
SHROOM4 participates in the regulation of cell morphogenesis particularly in the development of polarized cellular structures. SHROOM4 often associates with the actomyosin complex thereby influencing cell shape and polarity. By participating in these cellular processes SHROOM4 affects the organization and positioning of cells within tissues contributing to the proper formation of organ structures during development. Its interactions with other members of the SHROOM protein family enable it to execute its functions within the cellular architecture effectively.
- Pathways
SHROOM4 is involved in the planar cell polarity pathway which is essential for coordinated orientation and arrangement of cells in the epithelial layer. This pathway also includes other key proteins like VANGL2. Besides SHROOM4 contributes to the actin cytoskeleton organization pathway where it engages with proteins like RHOA to modify actin dynamics. These interactions ensure precise cellular alignment and positioning critical for tissue and organ architecture during embryonic development.
- Associated diseases and disorders
SHROOM4 has connections to neurodevelopmental disorders such as epilepsy. Mutations in SHROOM4 correlate with disruption in neural circuit formation contributing to epileptic manifestations. Additionally SHROOM4's role in cytoarchitecture links it with conditions like X-linked intellectual disability. Proteins like ARHGEF6 accompany SHROOM4 in the disease mechanisms highlighting its involvement in maintaining cellular integrity and function in neural cells. Study of these associations reveals SHROOM4's significance in neurological health and disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-SHROOM4 antibody (ab121316)
ab1216316, at 4μg/ml, staining SHROOM4 in in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHROOM4 antibody (ab121316)
ab121316, at 1/50 dilution, staining SHROOM4 in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
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