Anti-Shugoshin antibody [3C11]

• Flow Cyt

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Flow Cytometry - Anti-Shugoshin antibody [3C11] (AB58023)

Overlay histogram showing MCF-7 cells stained with ab58023 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58023, 0.5μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

This image was generated using the ascites version of the product.

• WB

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Western blot - Anti-Shugoshin antibody [3C11] (AB58023)

Western blot against tagged recombinant protein immunogen using ab58023 Shugoshin antibody at 1ug/ml. Predicted band size of immunogen is 58 kDa.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Shugoshin antibody [3C11] (ab58023)

Predicted band size: 64 kDa

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