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AB224085

Anti-SI antibody

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(1 Publication)

Rabbit Polyclonal SI antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SI aa 1-200.

View Alternative Names

SI

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (AB224085)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (AB224085)

Immunohistochemical analysis of human stomach tissue labeling SI with ab224085 at 1/200 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (AB224085)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (AB224085)

Immunohistochemical analysis of human small intestine tissue labeling SI with ab224085 at 1/200 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (AB224085)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (AB224085)

Immunohistochemical analysis of human colon tissue labeling SI with ab224085 at 1/200 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (AB224085)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (AB224085)

Immunohistochemical analysis of human small intestine tissue labeling SI with ab224085 at 1/200 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-SI antibody (AB224085)
  • WB

Unknown

Western blot - Anti-SI antibody (AB224085)

All lanes:

ab224085 at 0.4 µg/mL

All lanes:

Human small intestine tissue lysate

Predicted band size: 209 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SI aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P14410

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SI also known as sucrase-isomaltase is an enzyme complex with a molecular mass of approximately 245 kDa. It resides mainly in the brush border membrane of the small intestine. SI facilitates the digestion of dietary carbohydrates by splitting sucrose and isomaltase into simpler monosaccharides. This enzyme plays an important role in nutrient absorption and carbohydrate metabolism ensuring the small intestine can effectively process ingested sugars.
Biological function summary

SI serves as a critical mediator in carbohydrate metabolism. It consists of two subunits sucrase and isomaltase working in tandem to hydrolyze disaccharides. Each subunit contributes its specific catalytic activity to the breakdown of complex sugars. Enzymatic activity of SI is essential for the small intestine's ability to efficiently convert disaccharides into glucose and fructose. The localization of SI in the intestinal brush border maximizes its contact with ingested carbohydrates facilitating their rapid digestion and ensuing absorption.

Pathways

SI is integral to digestive pathways involving carbohydrate processing. It actively participates in the starch and sucrose metabolism pathway interacting with enzymes such as lactase and maltase which also hydrolyze carbohydrates. This connection underpins SI's contribution to the energy homeostasis by ensuring efficient carbohydrate digestion. Proper function of this pathway supports maintaining adequate glucose levels in the body highlighting its significant role in energy metabolism.

Mutations or deficiencies in SI lead to conditions like congenital sucrase-isomaltase deficiency (CSID) which results in carbohydrate malabsorption and gastrointestinal distress. Disorders related to SI often involve other proteins like lactase which also affect carbohydrate digestion. Understanding the relationship between SI and these digestive disorders aids in developing therapies and dietary adjustments aiming to alleviate symptoms associated with carbohydrate malabsorption.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in the final stage of carbohydrate digestion. Isomaltase activity is specific for both alpha-1,4- and alpha-1,6-oligosaccharides.
See full target information SI
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35008858

2021

New Proteins Contributing to Immune Cell Infiltration and Pannus Formation of Synovial Membrane from Arthritis Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Dominique de Seny,Dominique Baiwir,Elettra Bianchi,Gaël Cobraiville,Céline Deroyer,Christophe Poulet,Olivier Malaise,Geneviève Paulissen,Marie-Joëlle Kaiser,Jean-Philippe Hauzeur,Gabriel Mazzucchelli,Philippe Delvenne,Michel Malaise
View all publications
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