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Rabbit Polyclonal SI antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SI aa 1-200.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human SI aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P14410
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/200.00000 - 1/500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Target data

Function

Plays an important role in the final stage of carbohydrate digestion. Isomaltase activity is specific for both alpha-1,4- and alpha-1,6-oligosaccharides.

Alternative names

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Rabbit Polyclonal SI antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SI aa 1-200.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human SI aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P14410
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

SI serves as a critical mediator in carbohydrate metabolism. It consists of two subunits sucrase and isomaltase working in tandem to hydrolyze disaccharides. Each subunit contributes its specific catalytic activity to the breakdown of complex sugars. Enzymatic activity of SI is essential for the small intestine's ability to efficiently convert disaccharides into glucose and fructose. The localization of SI in the intestinal brush border maximizes its contact with ingested carbohydrates facilitating their rapid digestion and ensuing absorption.

Activity summary

SI also known as sucrase-isomaltase is an enzyme complex with a molecular mass of approximately 245 kDa. It resides mainly in the brush border membrane of the small intestine. SI facilitates the digestion of dietary carbohydrates by splitting sucrose and isomaltase into simpler monosaccharides. This enzyme plays an important role in nutrient absorption and carbohydrate metabolism ensuring the small intestine can effectively process ingested sugars.

Pathways

SI is integral to digestive pathways involving carbohydrate processing. It actively participates in the starch and sucrose metabolism pathway interacting with enzymes such as lactase and maltase which also hydrolyze carbohydrates. This connection underpins SI's contribution to the energy homeostasis by ensuring efficient carbohydrate digestion. Proper function of this pathway supports maintaining adequate glucose levels in the body highlighting its significant role in energy metabolism.

Associated diseases and disorders

Mutations or deficiencies in SI lead to conditions like congenital sucrase-isomaltase deficiency (CSID) which results in carbohydrate malabsorption and gastrointestinal distress. Disorders related to SI often involve other proteins like lactase which also affect carbohydrate digestion. Understanding the relationship between SI and these digestive disorders aids in developing therapies and dietary adjustments aiming to alleviate symptoms associated with carbohydrate malabsorption.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-SI antibody (ab224085), expandable thumbnail

    Western blot - Anti-SI antibody (ab224085)

    All lanes: ab224085 at 0.4 µg/mL

    All lanes: Human small intestine tissue lysate

    Predicted band size: 209 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (ab224085), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (ab224085)

    Immunohistochemical analysis of human small intestine tissue labeling SI with ab224085 at 1/200 dilution.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (ab224085), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (ab224085)

    Immunohistochemical analysis of human small intestine tissue labeling SI with ab224085 at 1/200 dilution.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (ab224085), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (ab224085)

    Immunohistochemical analysis of human stomach tissue labeling SI with ab224085 at 1/200 dilution. No positivity as expected.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (ab224085), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (ab224085)

    Immunohistochemical analysis of human colon tissue labeling SI with ab224085 at 1/200 dilution. No positivity as expected.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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