Rat Recombinant Monoclonal SI-CLP antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.
Images
![Western blot - Anti-SI-CLP antibody [1B8] - BSA and Azide free (AB305177), expandable thumbnail](https://content.abcam.com/products/images/si-clp-antibody-1b8-bsa-and-azide-free-ab305177--western-blot-img404507.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SI-CLP antibody [1B8] - BSA and Azide free (AB305177)")
![Western blot - Anti-SI-CLP antibody [1B8] - BSA and Azide free (AB305177), expandable thumbnail](https://content.abcam.com/products/images/si-clp-antibody-1b8-bsa-and-azide-free-ab305177--western-blot-img404506.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SI-CLP antibody [1B8] - BSA and Azide free (AB305177)")
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | IP | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Saccharide- and LPS-binding protein with possible roles in pathogen sensing and endotoxin neutralization. Ligand-binding specificity relates to the length of the oligosaccharides, with preference for chitotetraose (in vitro).
Alternative names
GL008, PSEC0104, SB139, CHID1, Chitinase domain-containing protein 1, Stabilin-1-interacting chitinase-like protein, SI-CLP
Recommended products
Rat Recombinant Monoclonal SI-CLP antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 1B8
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab305177 is the carrier-free version of Anti-SI-CLP antibody [1B8] ab305176.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SI-CLP also known as ClpB or Caseinolytic Peptidase B is an ATP-dependent protease with a molecular mass of approximately 95 kDa. It plays a critical role in disaggregating proteins facilitating their refolding in stress conditions. The target is mainly expressed in prokaryotic organisms and some eukaryotic mitochondria. Its mechanism involves using energy derived from ATP hydrolysis to remodel protein aggregates making it essential for maintaining cellular protein homeostasis.
- Biological function summary
SI-CLP proteins interact with other chaperone systems to assist in the recovery of proteins from aggregates. It forms part of a larger Clp/Hsp100 chaperone complex working together with co-chaperones like DnaK and GroEL to efficiently manage protein misfolding. This association with other molecular chaperones allows SI-CLP to execute its role in cellular stress response ensuring damaged or misfolded proteins are effectively processed or refolded.
- Pathways
SI-CLP contributes significantly within the cellular protein quality control and stress response pathways. In these pathways it closely associates with DnaK facilitating protein quality control under stress conditions. Related proteins such as Hsp70 also engage in similar pathways highlighting their coordinated roles in preserving cellular function especially during stress.
- Associated diseases and disorders
SI-CLP proteins have associations with neurodegenerative diseases like Alzheimer’s disease where protein aggregation occurs. It connects with proteins involved in managing protein aggregates such as Hsp70. Furthermore its role in cellular stress responses implicates it in some infectious diseases where pathogens hijack host protein quality control systems. The involvement of SI-CLP in these disorders highlights its potential as a therapeutic target to modulate protein aggregation-related pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-SI-CLP antibody [1B8] - BSA and Azide free (ab305177), expandable thumbnail](https://content.abcam.com/products/images/si-clp-antibody-1b8-bsa-and-azide-free-ab305177--western-blot-img404507.jpg "Western blot - Anti-SI-CLP antibody [1B8] - BSA and Azide free (ab305177)")
Western blot - Anti-SI-CLP antibody [1B8] - BSA and Azide free (ab305177)
This data was developed using Anti-SI-CLP antibody [1B8] ab305176, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time:
Lanes 1-4: 92 seconds
Lanes 5/7-9: 3 minutes
Lane 6: 10 secondsAll lanes: Western blot - Anti-SI-CLP antibody [1B8] (Anti-SI-CLP antibody [1B8] ab305176) at 1/1000 dilution
Lane 1: Mouse spleen lysate at 20 µg
Lane 2: Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate at 20 µg
Lane 3: THP-1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg
Lane 4: U937 (human histiocytic lymphoma monocyte), whole cell lysate at 20 µg
Lane 5: Mouse pancreas lysate at 20 µg
Lane 6: Mouse testis lysate at 20 µg
Lane 7: A20 (mouse reticum sarcoma B lymphocyte), whole cell lysate 20 at 20 µg
Lane 8: EL4 (mouse lymphoma T lymphocyte), whole cell lysate at 20 µg
Lane 9: EL4.IL-2 (mouse lymphoma T lymphocyte), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution
Observed band size: 44 kDa
![Western blot - Anti-SI-CLP antibody [1B8] - BSA and Azide free (ab305177), expandable thumbnail](https://content.abcam.com/products/images/si-clp-antibody-1b8-bsa-and-azide-free-ab305177--western-blot-img404506.jpg "Western blot - Anti-SI-CLP antibody [1B8] - BSA and Azide free (ab305177)")
Western blot - Anti-SI-CLP antibody [1B8] - BSA and Azide free (ab305177)
This data was developed using Anti-SI-CLP antibody [1B8] ab305176, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 62 seconds.All lanes: Western blot - Anti-SI-CLP antibody [1B8] (Anti-SI-CLP antibody [1B8] ab305176) at 1/1000 dilution
Lane 1: THP-1 (human monocytic leukemia monocyte) transfected with scrambled siRNA control, whole cell lysate at 20 µg
Lane 2: THP-1 transfected with siRNA specifically targeti SI-CLP, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution
Observed band size: 44 kDa
Exposure time: 62s
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