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AB69300

Anti-SIAE antibody

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(1 Publication)

Mouse Polyclonal SIAE antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SIAE.
1 Images
Western blot - Anti-SIAE antibody (AB69300)
  • WB

Unknown

Western blot - Anti-SIAE antibody (AB69300)

All lanes:

Western blot - Anti-SIAE antibody (ab69300) at 1/500 dilution

All lanes:

human colon tissue lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 58 kDa

Observed band size: 21 kDa,26 kDa,32 kDa,35 kDa,58 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SIAE.

Q9HAT2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sialic acid acetylesterase also known as SIAE is an enzyme that functions by removing acetyl groups from sialic acids on glycoconjugates. This enzyme belongs to the esterase family and exhibits a mass of approximately 60 kDa. SIAE shows expression in various tissues including the liver kidney and brain. It operates by breaking these acetyl linkages which is an important step in regulating sialic acid biology.
Biological function summary

SIAE modifies the sialylation of glycoconjugates which impacts cellular recognition and communication. This enzyme is part of the immune regulation complex influencing how immune cells recognize and interact with each other as well as their environment. SIAE’s activity can alter cell surface properties which affects processes such as cell adhesion migration and signaling.

Pathways

This enzyme appears in sialic acid metabolic pathways and immune response pathways. Within the context of the sialic acid metabolism pathway it works collaboratively with other enzymes like NEU1 and ST6GAL1 impacting the breakdown and synthesis of sialic acid derivatives. Additionally within immune response pathways SIAE influences the function of proteins involved in the regulation of immune cell signaling thereby affecting adaptive and innate immunity.

Researchers have connected SIAE with autoimmune diseases and certain neurodevelopmental disorders. Mutations or dysregulation in SIAE have been linked to conditions like systemic lupus erythematosus (SLE) and autism spectrum disorder (ASD). In the context of SLE SIAE's interaction with proteins like CTLA-4 impacts immune tolerance and autoreactivity. In ASD abnormal sialic acid modifications have implications for neurodevelopmental processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the removal of O-acetyl ester groups from position 9 of the free diacetylated sialate N-acetyl-9-O-acetylneuraminate (Neu5,9Ac2) in the cytosol and of the diacetylated sialate residues of sialylglycoconjugates in the lysosomes (Probable). Together with the sialate-O-acetyltransferase they regulate the balance of acetylated sialoglycoconjugates, key players in various processes such as cell-cell interactions, host-pathogen recognition, and tumor antigenicity (PubMed : 21803834).
See full target information SIAE

Alternative Names

YSG2, SIAE, Sialate O-acetylesterase, H-Lse, Sialic acid-specific 9-O-acetylesterase
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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

Molecular therapy. Nucleic acids 29:969-978 PubMed36189081

2022

Anti-TGF-β1 aptamer enhances therapeutic effect of tyrosine kinase inhibitor, gefitinib, on non-small cell lung cancer in xenograft model.

Applications

Unspecified application

Species

Unspecified reactive species

Masaki Takahashi,Yoshifumi Hashimoto,Yoshikazu Nakamura
View all publications
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Product promise

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