Rabbit Recombinant Monoclonal SIAH1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-SIAH1 antibody [EPR27101-45] - BSA and Azide free (AB305250), expandable thumbnail](https://content.abcam.com/products/images/siah1-antibody-epr27101-45-bsa-and-azide-free-ab305250--western-blot-img405199.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SIAH1 antibody [EPR27101-45] - BSA and Azide free (AB305250)")
![Western blot - Anti-SIAH1 antibody [EPR27101-45] - BSA and Azide free (AB305250), expandable thumbnail](https://content.abcam.com/products/images/siah1-antibody-epr27101-45-bsa-and-azide-free-ab305250--western-blot-img405198.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SIAH1 antibody [EPR27101-45] - BSA and Azide free (AB305250)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | ICC/IF | IHC-P | WB | IP | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:14506261, PubMed:14645235, PubMed:14654780, PubMed:15064394, PubMed:16085652, PubMed:19224863, PubMed:20508617, PubMed:22483617, PubMed:28546513, PubMed:32430360, PubMed:33591310, PubMed:9334332, PubMed:9858595). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:14506261, PubMed:14645235, PubMed:14654780, PubMed:15064394, PubMed:16085652, PubMed:19224863, PubMed:20508617, PubMed:22483617, PubMed:9334332, PubMed:9858595). Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes (PubMed:14506261, PubMed:14645235, PubMed:14654780, PubMed:15064394, PubMed:16085652, PubMed:19224863, PubMed:20508617, PubMed:22483617, PubMed:9334332, PubMed:9858595). Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP (PubMed:10747903, PubMed:11146551, PubMed:11389839, PubMed:11389840, PubMed:11483517, PubMed:11483518, PubMed:11752454, PubMed:12072443). Confers constitutive instability to HIPK2 through proteasomal degradation (PubMed:18536714, PubMed:33591310). It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling (PubMed:14506261, PubMed:14645235, PubMed:14654780, PubMed:15064394, PubMed:16085652, PubMed:19224863, PubMed:20508617, PubMed:22483617, PubMed:9334332, PubMed:9858595). Has some overlapping function with SIAH2 (PubMed:14506261, PubMed:14645235, PubMed:14654780, PubMed:15064394, PubMed:16085652, PubMed:19224863, PubMed:20508617, PubMed:22483617, PubMed:9334332, PubMed:9858595). Induces apoptosis in cooperation with PEG3 (By similarity). Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus (By similarity). GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins (By similarity). Mediates ubiquitination and degradation of EGLN2 and EGLN3 in response to the unfolded protein response (UPR), leading to their degradation and subsequent stabilization of ATF4 (By similarity). Also part of the Wnt signaling pathway in which it mediates the Wnt-induced ubiquitin-mediated proteasomal degradation of AXIN1 (PubMed:28546513, PubMed:32430360).
Alternative names
HUMSIAH, SIAH1, E3 ubiquitin-protein ligase SIAH1, RING-type E3 ubiquitin transferase SIAH1, Seven in absentia homolog 1, Siah-1a, Siah-1
Recommended products
Rabbit Recombinant Monoclonal SIAH1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27101-45
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SIAH1 also known as Seven In Absentia Homolog 1 functions mechanically as a RING-type E3 ubiquitin-protein ligase. This protein weighs approximately 32 kDa and adds ubiquitin to target proteins tagging them for proteasomal degradation. Researchers have found SIAH1 expressed in various tissue types including brain heart and kidney. By regulating protein stability SIAH1 plays a significant role in maintaining cellular protein homeostasis.
- Biological function summary
SIAH1 influences cell cycle control and apoptosis by interacting with components of protein complexes. It associates with the ubiquitin-proteasome pathway to mediate degradation of key regulatory proteins. SIAH1 helps in reducing cellular proliferation and enhances apoptotic signaling which are vital processes for maintaining balance between cell survival and death. As part of these processes studies have connected SIAH1 to modulating the fate of proteins involved in cell cycle arrest.
- Pathways
Several interactions of SIAH1 contribute to signal transduction and apoptosis. One significant pathway involving SIAH1 is the p53 signaling pathway. SIAH1 regulates the degradation of proteins within this pathway including p21 and cyclin D1 which influence cellular responses to DNA damage. Additionally SIAH1 interacts with β-catenin linking it to the Wnt signaling pathway. This interaction affects the regulation of cell growth and differentiation.
- Associated diseases and disorders
SIAH1 has links to cancer and neurodegenerative diseases. In cancer altered SIAH1 expression affects tumor suppressor activity through its interaction with p53 which can lead to uncontrolled cell growth. Moreover SIAH1 connects to neurodegenerative disorders like Parkinson's disease where abnormal protein degradation contributes to neuronal death. In such contexts researchers observe altered interactions between SIAH1 and proteins like parkin a ubiquitin ligase also implicated in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-SIAH1 antibody [EPR27101-45] - BSA and Azide free (ab305250), expandable thumbnail](https://content.abcam.com/products/images/siah1-antibody-epr27101-45-bsa-and-azide-free-ab305250--western-blot-img405199.jpg "Western blot - Anti-SIAH1 antibody [EPR27101-45] - BSA and Azide free (ab305250)")
Western blot - Anti-SIAH1 antibody [EPR27101-45] - BSA and Azide free (ab305250)
This data was developed using Anti-SIAH1 antibody [EPR27101-45] ab305249, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 48 secondsAll lanes: Western blot - Anti-SIAH1 antibody [EPR27101-45] (Anti-SIAH1 antibody [EPR27101-45] ab305249) at 1/1000 dilution
Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 20 μg
Lane 2: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Observed band size: 31 kDa
Exposure time: 48s
![Western blot - Anti-SIAH1 antibody [EPR27101-45] - BSA and Azide free (ab305250), expandable thumbnail](https://content.abcam.com/products/images/siah1-antibody-epr27101-45-bsa-and-azide-free-ab305250--western-blot-img405198.jpg "Western blot - Anti-SIAH1 antibody [EPR27101-45] - BSA and Azide free (ab305250)")
Western blot - Anti-SIAH1 antibody [EPR27101-45] - BSA and Azide free (ab305250)
This data was developed using Anti-SIAH1 antibody [EPR27101-45] ab305249, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 70 secondsAll lanes: Western blot - Anti-SIAH1 antibody [EPR27101-45] (Anti-SIAH1 antibody [EPR27101-45] ab305249) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate 20 μg
Lane 2: 293T transfected with siRNA specifically targeting SIAH1 whole cell lysate, 20 ug
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Observed band size: 31 kDa
Exposure time: 70s
Downloads
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