Anti-Sialoadhesin/CD169 antibody [EPR27102-11]

14 product images

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  Multiplex immunohistochemistry - Anti-Sialoadhesin/CD169 antibody [EPR27102-11] (ab312840)

  Sialoadhesin/CD169 Multiplex immunohistochemistry staining of Rat spleen using rabbit Anti-Sialoadhesin/CD169 antibody

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat spleen tissue labeling Sialoadhesin/CD169,CD3 epsilon and CD20 with ab312840 at 1/100 dilution, Anti-CD3 epsilon antibody [SP7] ab16669 at 1:150 dilution and Anti-CD20 antibody [SP32] - BSA and Azide free ab236434 at 1:5000 dilution.
  
  
  Panel A: merged staining of anti-Sialoadhesin/CD169 (red; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD20 (gray; Opal™570) on rat spleen.
  Panel B: anti-Sialoadhesin/CD169 stained on macrophages.
  Panel C: anti-CD3 epsilon stained on T cells.
  Panel D: anti-CD20 stained on B cells.
  
  The section was incubated in three rounds of staining: in the order of ab312840, Anti-CD3 epsilon antibody [SP7] ab16669, and Anti-CD20 antibody [SP32] - BSA and Azide free ab236434 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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  Multiplex immunohistochemistry - Anti-Sialoadhesin/CD169 antibody [EPR27102-11] (ab312840)

  Sialoadhesin/CD169 Multiplex immunohistochemistry staining of Mouse spleen using rabbit Anti-Sialoadhesin/CD169 antibody

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse spleen tissue labeling Sialoadhesin/CD169, CD3 epsilon and CD20 with ab312840 at 1/100 dilution, Anti-CD3 epsilon antibody [SP7] ab16669 at 1:150 dilution and Anti-CD20 antibody [SP32] - BSA and Azide free ab236434 at 1:5000 dilution.
  
  Panel A: merged staining of anti-Sialoadhesin/CD169 (red; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD20 (gray; Opal™570) on mouse spleen.
  Panel B: anti-Sialoadhesin/CD169 stained on macrophages.
  Panel C: anti-CD3 epsilon stained on T cells.
  Panel D: anti-CD20 stained on B cells.
  
  The section was incubated in three rounds of staining: in the order of ab312840, Anti-CD3 epsilon antibody [SP7] ab16669, and Anti-CD20 antibody [SP32] - BSA and Azide free ab236434 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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  Western blot - Anti-Sialoadhesin/CD169 antibody [EPR27102-11] (ab312840)

  Sialoadhesin/CD169 Western blot staining using rabbit Anti-Sialoadhesin/CD169 antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  Low expression: kidney (PMID 33408860).
  This blot of Lane 6 was developed using a high sensitivity ECL substrate.
  In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) loading control staining at 1/10000 dilution.
  Exposure time: Lane 1-5: 180 seconds; Lane 6: 114 seconds.

  All lanes: Western blot - Anti-Sialoadhesin/CD169 antibody [EPR27102-11] (ab312840) at 1/1000 dilution

  Lane 1: Mouse spleen tissue lysate at 20 µg

  Lane 2: Mouse lymph node tissue lysate at 20 µg

  Lane 3: Mouse kidney tissue lysate at 20 µg

  Lane 4: Rat lymph node tissue lysate at 20 µg

  Lane 5: Rat kidney tissue lysate at 20 µg

  Lane 6: Rat spleen tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 250 kDa

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  Western blot - Anti-Sialoadhesin/CD169 antibody [EPR27102-11] (ab312840)

  Sialoadhesin/CD169 Western blot staining using rabbit Anti-Sialoadhesin/CD169 antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  The expression of Sialoadhesin/CD169 is upregulated in response to LPS treatment (PMID: 27129263).
  In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) loading control staining at 1/10000 dilution.
  Exposure time: 180 seconds

  All lanes: Western blot - Anti-Sialoadhesin/CD169 antibody [EPR27102-11] (ab312840) at 1/1000 dilution

  Lane 1: Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

  Lane 2: RAW 264.7 treated with /ml LPS for 16h whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 250 kDa

  Exposure time: 180s

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  Immunocytochemistry/ Immunofluorescence - Anti-Sialoadhesin/CD169 antibody [EPR27102-11] (ab312840)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) cells labelling Sialoadhesin/CD169 with ab312840 at 1/250 (2.064 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing positive staining in RAW 264.7 cells treated with lipopolysaccharide (100ng/ml) for 16 hours.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [EPR27102-11] (ab312840)

  Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Sialoadhesin/CD169 with ab312840 at 1/100 (5.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat spleen interfollicular zone. The section was incubated with ab312840 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [EPR27102-11] (ab312840)

  Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling Sialoadhesin/CD169 with ab312840 at 1/100 (5.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on mouse skeletal muscle. The section was incubated with ab312840 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [EPR27102-11] (ab312840)

  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Sialoadhesin/CD169 with ab312840 at 1/100 (5.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on mouse kidney. The section was incubated with ab312840 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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  Flow Cytometry - Anti-Sialoadhesin/CD169 antibody [EPR27102-11] (ab312840)

  Flow cytometric analysis of Mouse bone marrow cells labelling Sialoadhesin/CD169 with ab312840 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Cells co-stained with F4/80 conjugated to PE. Gated on viable cells.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [EPR27102-11] (ab312840)

  Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Sialoadhesin/CD169 with ab312840 at 1/100 (5.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat kidney. The section was incubated with ab312840 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [EPR27102-11] (ab312840)

  Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Sialoadhesin/CD169 with ab312840 at 1/100 (5.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse spleen interfollicular zone (PMID: 15107454). The section was incubated with ab312840 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [EPR27102-11] (ab312840)

  Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Sialoadhesin/CD169 with ab312840 at 1/100 (5.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat skeletal muscle. The section was incubated with ab312840 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Flow Cytometry - Anti-Sialoadhesin/CD169 antibody [EPR27102-11] (ab312840)

  Flow cytometric analysis of Mouse bone marrow cells labelling Sialoadhesin/CD169 with ab312840 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Cells co-stained with CD3 conjugated to A647. Gated on viable cells.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [EPR27102-11] (ab312840)

  Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human tissue labeling Sialoadhesin/CD169 with ab312840 at 1/2000 (0.258 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with a mouse SIGLEC1 expression vector containing a his tag. No staining on (B) HEK-293T transfected with empty vector containing a his tag. The section was incubated with ab312840 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.