Anti-Sialoadhesin/CD169 antibody [EPR30523-549]

• Flow Cyt

Lab

Flow Cytometry - Anti-Sialoadhesin/CD169 antibody [EPR30523-549] (AB326438)

Flow cytometric analysis of Human PBMC isolated CD14+ monocytes treated with IFNa (1000 IU/ml ) for 24 hours (Lower left and right) Human PBMC (human peripheral blood mononuclear cell) isolated CD14+ monocytes (Upper left and right) cells labelling Sialoadhesin/CD169 with ab326438 at 1/50 dilution (1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-CD14 conjugated to Brilliant Violet 421.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Sialoadhesin/CD169 antibody [EPR30523-549] (AB326438)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC (human peripheral blood mononuclear cell) isolated CD14+ monocytes Human PBMC isolated CD14+ monocytes treated with IFNa (1000 IU/ml ) for 24 hours Human CD14 depleted PMBC Human CD14 depleted PMBC treated with IFNa (1000 IU/ml ) for 24 hours cells labelling Sialoadhesin/CD169 with ab326438 at 1/50 (10.1 µg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing membranous and cytoplasmic staining in Human PBMC isolated CD14+ monocytes treated with IFNa (1000 IU/ml ) for 24 hours(shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). A : Human PBMC (human peripheral blood mononuclear cell) isolated CD14+ monocytes B : Human PBMC isolated CD14+ monocytes treated with IFNa (1000 IU/ml ) for 24 hours C : Human CD14 depleted PMBC D : Human CD14 depleted PMBC treated with IFNa (1000 IU/ml ) for 24 hours

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• Flow Cyt

Lab

Flow Cytometry - Anti-Sialoadhesin/CD169 antibody [EPR30523-549] (AB326438)

Flow cytometric analysis of Human CD14 depleted PMBC treated with IFNa (1000 IU/ml ) for 24 hours (Lower left and right) Human CD14 depleted PMBC (Upper left and right) cells labelling Sialoadhesin/CD169 with ab326438 at 1/50 dilution (1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-CD3 conjugated to Brilliant Violet 421.