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Mouse Monoclonal Sialoadhesin/CD169 antibody. Carrier free. Suitable for WB, IHC-Fr and reacts with Human samples.

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Images

Immunohistochemistry (Frozen sections) - Anti-Sialoadhesin/CD169 antibody [HSn 7D2] - BSA and Azide free (AB269566), expandable thumbnail
  • Western blot - Anti-Sialoadhesin/CD169 antibody [HSn 7D2] - BSA and Azide free (AB269566), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-Fr
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg/mL
Notes

-

Associated Products

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Target data

Function

Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Plays a crucial role in limiting bacterial dissemination by engaging sialylated bacteria to promote effective phagocytosis and antigen presentation for the adaptive immune response (PubMed:12940982, PubMed:33489013). Mediates the uptake of various enveloped viruses via sialic acid recognition and subsequently induces the formation of intracellular compartments filled with virions (VCCs) (PubMed:28129379). In turn, enhances macrophage-to-T-cell transmission of several viruses including HIV-1 or SARS-CoV-2 (PubMed:28129379, PubMed:34782760). Acts as an endocytic receptor mediating clathrin dependent endocytosis. Preferentially binds to alpha-2,3-linked sialic acid (PubMed:12940982). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. Plays a role in the inhibition of antiviral innate immune by promoting TBK1 degradation via TYROBP and TRIM27-mediated ubiquitination (PubMed:26358190). (Microbial infection) Facilitates viral cytoplasmic entry into activated dendritic cells via recognition of sialylated gangliosides pesent on viral membrane.

Alternative names

CD169, SN, SIGLEC1, Sialoadhesin, Sialic acid-binding Ig-like lectin 1, Siglec-1

Recommended products

Mouse Monoclonal Sialoadhesin/CD169 antibody. Carrier free. Suitable for WB, IHC-Fr and reacts with Human samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
HSn 7D2
Purification technique
Affinity purification Protein G
Epitope
Within domains 1-4.
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab269566 is the carrier-free version of Anti-Sialoadhesin/CD169 antibody [HSn 7D2] ab18619.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Frozen sections) - Anti-Sialoadhesin/CD169 antibody [HSn 7D2] - BSA and Azide free (ab269566), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Sialoadhesin/CD169 antibody [HSn 7D2] - BSA and Azide free (ab269566)

    IHC image of Sialoadhesin/CD169 staining in human liver frozen tissue section, fixed in 10% paraformaldehyde (10 min). Staining was performed on a Leica Bond system using the standard protocol F. The section was then incubated with Anti-Sialoadhesin/CD169 antibody [HSn 7D2] ab18619, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This image was produced using the same antibody clone but in a different formulation Anti-Sialoadhesin/CD169 antibody [HSn 7D2] ab18619, PBS and sodium azide.

  • Western blot - Anti-Sialoadhesin/CD169 antibody [HSn 7D2] - BSA and Azide free (ab269566), expandable thumbnail

    Western blot - Anti-Sialoadhesin/CD169 antibody [HSn 7D2] - BSA and Azide free (ab269566)

    This blot was produced using a 3-8% Tris-Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% BSA before being incubated with ab269566 overnight at 4°C. Antibody binding was detected using a Goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - Anti-Sialoadhesin/CD169 antibody [HSn 7D2] (Anti-Sialoadhesin/CD169 antibody [HSn 7D2] ab18619) at 1 µg/mL

    Lane 1: Human spleen whole tissue lysate at 30 µg

    Lane 2: Human lymph node whole tissue lysate at 30 µg

    Secondary

    All lanes: HRP conjugated Goat Anti-Mouse IgG (H+L) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 182 kDa

    Exposure time: 20min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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