AB227672

Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal

Name: Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672) | Abcam
Brand: Abcam Limited
SKU: AB227672
Price: 190 USD
Availability: InStock
Rating: 5 (1 reviews)

BOND RX™ Validated
20ul selling size
RabMAb
Advanced Validation
Recombinant
What is this?

(1 Review)

(1 Publication)

Rabbit Recombinant Monoclonal Sialoadhesin/CD169 antibody. C-terminal. Suitable for Flow Cyt (Intra), IHC-P, mIHC and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD169, SN, SIGLEC1, Sialoadhesin, Sialic acid-binding Ig-like lectin 1, Siglec-1

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Formalin-fixed, paraffin-embedded human colon tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Sialoadhesin/CD169 with ab227672 at 1/100 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Formalin-fixed paraffin-embedded human adrenal gland tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Formalin-fixed paraffin-embedded human lung tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Formalin-fixed, paraffin-embedded human liver tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Flow Cytometry analysis of Human PBMC treated with 20ng/ml IFN-gamma for 24 hours cells labeling Sialoadhesin/CD169 with purified ab227672 at 1/20 dilution (6 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730). Unlabeled control - Unlabelled cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Formalin-fixed paraffin-embedded human bone marrow tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Sialoadhesin/CD169 with ab227672 at 1/100 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Multiplex immunohistochemistry - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

mIHC

Lab

Multiplex immunohistochemistry - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human lung tissue. Panel A : Merged staining of anti-Podoplanin / gp36 (magenta; Opal™690), anti-Surfactant protein D/SP-D (green; Opal™520) and anti-Sialoadhesin/CD169 (red; Opal™570) on human lung. Panel B : Anti-Podoplanin / gp36 stained on alveolar type I cells. Panel C : Anti-Surfactant protein D/SP-D stained on alveolar type II cells. Panel D : Anti-Sialoadhesin/CD169 stained on macrophages. The section was incubated in three rounds of staining : in the order of ab236529 and ab234257 for 30 mins, then ab245735 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Counterstained with DAPI.

Carrier free

Anti-Sialoadhesin/CD169 antibody [SP213] - BSA and Azide free
660 APC

APC Anti-Sialoadhesin antibody [SP213] (C-terminal)
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Sialoadhesin antibody [SP213] - C-terminal
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Sialoadhesin antibody [SP213] - C-terminal
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Sialoadhesin antibody [SP213] - C-terminal
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Sialoadhesin antibody [SP213] - C-terminal
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal
HRP

HRP Anti-Sialoadhesin antibody [SP213] (C-terminal)
578 PE

PE Anti-Sialoadhesin antibody [SP213] (C-terminal)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP213

Isotype

IgG

Carrier free

Reacts with

Human

Applications

mIHC, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "" } } }

style

left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Plays a crucial role in limiting bacterial dissemination by engaging sialylated bacteria to promote effective phagocytosis and antigen presentation for the adaptive immune response (PubMed : 12940982, PubMed : 33489013). Mediates the uptake of various enveloped viruses via sialic acid recognition and subsequently induces the formation of intracellular compartments filled with virions (VCCs) (PubMed : 28129379). In turn, enhances macrophage-to-T-cell transmission of several viruses including HIV-1 or SARS-CoV-2 (PubMed : 28129379, PubMed : 34782760). Acts as an endocytic receptor mediating clathrin dependent endocytosis. Preferentially binds to alpha-2,3-linked sialic acid (PubMed : 12940982). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. Plays a role in the inhibition of antiviral innate immune by promoting TBK1 degradation via TYROBP and TRIM27-mediated ubiquitination (PubMed : 26358190).. (Microbial infection) Facilitates viral cytoplasmic entry into activated dendritic cells via recognition of sialylated gangliosides pesent on viral membrane.

See full target information SIGLEC1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature medicine 31:1949-1957 PubMed40082695

2025

A B7H3-targeting antibody-drug conjugate in advanced solid tumors: a phase 1/1b trial.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxiang Ma,Yunpeng Yang,Yan Huang,Wenfeng Fang,Jinhui Xue,Xiangjiao Meng,Yun Fan,Siqing Fu,Lin Wu,Yulong Zheng,Jian Liu,Zhihua Liu,Wu Zhuang,Seth Rosen,Song Qu,Bihui Li,Mingjun Li,Yanqiu Zhao,Shujun Yang,Yinghua Ji,David Sommerhalder,Suxia Luo,Kunyu Yang,Jingao Li,Dongqing Lv,Peng Zhang,Yuanyuan Zhao,Shaodong Hong,Yang Zhang,Shen Zhao,Steve Chin,Xian Zhang,Wei Lian,Jiaqiang Cai,Tongtong Xue,Li Zhang,Hongyun Zhao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Flow Cytometry (Intracellular) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Multiplex immunohistochemistry - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (AB227672)

Related conjugates and formulations (10)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise