Anti-Sialoadhesin/CD169 antibody [SP216]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] (AB183356)

Immunohistochemical analysis of formalin fixed, paraffin embedded human HK lymphoma tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Sialoadhesin/CD169 antibody [SP216] (AB183356)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human PBMC isolated CD14+ monocytes treated with 1000IU/ml IFNα for 24hours (Lower left and right) labelling Sialoadhesin/CD169 with ab183356 at 1/50 dilution (1ug)/ (Right) compared with a Rabbit monoclonal IgG (ab172730) / Left - Isotype Control (Left). Untreated Human PBMC isolated CD14+ monocytes (Upper left and right).

Cells blocked with 20ug/ml Human IgG.

Cells were surface stained with anti-CD14 conjugated to BV421. Then fixed with 4% PFA for 10 min followed by intracellular staining with rabbit IgG or our antibody.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] (AB183356)

Immunohistochemical analysis of formalin fixed, paraffin embedded human bone marrow tissue sections labeling Sialoadhesin/CD169 with ab183356 at a 1/100 dilution followed by rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.

Positive staining on the macrophages in the human bone marrow, performed on a Leica Biosystems BOND^® RX instrument.

The section was incubated with ab183356 for 30 mins at room temperature.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] (AB183356)

Immunohistochemical analysis of formalin fixed, paraffin embedded human lymph node tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] (AB183356)

Immunohistochemical analysis of formalin fixed, paraffin embedded human squamous cell lung carcinoma tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] (AB183356)

Immunohistochemical analysis of formalin fixed, paraffin embedded human squamous cell cervical carcinoma tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] (AB183356)

Immunohistochemical analysis of formalin fixed, paraffin embedded human bone marrow tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Sialoadhesin/CD169 antibody [SP216] (AB183356)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human PBMC MACS-CD14 neg treated with 1000IU/ml IFNα for 24hours (Lower left and right) labelling Sialoadhesin/CD169 with ab183356 at 1/50 dilution (1ug)/ (Right) compared with a Rabbit monoclonal IgG (ab172730) / Left - Isotype Control (Left). Untreated Human PBMC MACS-CD14 neg (Upper left and right)

Cells blocked with 20ug/ml Human IgG.

Cells were surface stained with anti-CD3 conjugated to BV421. Then fixed with 4% PFA for 10 min followed by intracellularly staining with rabbit IgG or our antibody. Gated on Lymphocytes