AB115957

Anti-Sialyl Tn antibody [STn 219]

Name: Anti-Sialyl Tn antibody [STn 219] Mouse monoclonal (ab115957) | Abcam
Brand: Abcam Limited
SKU: AB115957
Price: 420 USD
Availability: InStock

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(10 Publications)

Mouse Monoclonal Sialyl Tn antibody. Suitable for Flow Cyt and reacts with Human, Sheep samples. Cited in 10 publications.

View Alternative Names

CD175s, Sialosyl Tn, sTn

1 Images

Flow Cytometry - Anti-Sialyl Tn antibody [STn 219] (AB115957)

Flow Cyt

Unknown

Flow Cytometry - Anti-Sialyl Tn antibody [STn 219] (AB115957)

Overlay histogram showing MCF7 cells stained with ab115957 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115957, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

STn 219

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Sheep, Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

NeuAc a GalNacOSer/Thr.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Sheep": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 15th June 2017. The following lots are from ascites and still in stock as of 15th June 2017 (GR271829-1, GR293422-1, GR247695-1, GR293422-2, GR293422-1 ). Lot numbers higher than GR293422-2 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: 98% PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Sialyl Tn

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:3496 PubMed37311743

2023

Structural insights into Siglec-15 reveal glycosylation dependency for its interaction with T cells through integrin CD11b.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Pia Lenza,Leire Egia-Mendikute,Asier Antoñana-Vildosola,Cátia O Soares,Helena Coelho,Francisco Corzana,Alexandre Bosch,Prodhi Manisha,Jon Imanol Quintana,Iker Oyenarte,Luca Unione,María Jesús Moure,Mikel Azkargorta,Unai Atxabal,Klaudia Sobczak,Felix Elortza,James D Sutherland,Rosa Barrio,Filipa Marcelo,Jesús Jiménez-Barbero,Asis Palazon,June Ereño-Orbea

The EMBO journal 42:e111869 PubMed36245281

2022

The disulfide catalyst QSOX1 maintains the colon mucosal barrier by regulating Golgi glycosyltransferases.

Applications

Unspecified application

Species

Unspecified reactive species

Tal Ilani,Nava Reznik,Noa Yeshaya,Tal Feldman,Patrick Vilela,Zipora Lansky,Gabriel Javitt,Michal Shemesh,Ori Brenner,Yoav Elkis,Neta Varsano,Ana M Jaramillo,Christopher M Evans,Deborah Fass

Frontiers in molecular biosciences 9:985648 PubMed36172045

2022

Curcumin-mediated transcriptional regulation of human -acetylgalactosamine-α2,6-sialyltransferase which synthesizes sialyl-Tn antigen in HCT116 human colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

So-Young An,Kyoung-Sook Kim,Jong-Hyun Cho,Hee-Do Kim,Cheorl-Ho Kim,Young-Choon Lee

Molecular pharmacology 102:161-171 PubMed35764384

2022

Screening and Identification of a Novel Anti-Siglec-15 Human Antibody 3F1 and Relevant Antitumor Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaguo Wu,Jingyi Peng,Yangyihua Zhou,Ran Zhang,Zhihong Wang,Naijing Hu,Dingmu Zhang,Guiqi Quan,Yuanyu Wu,Jiannan Feng,Beifen Shen,Jian Zhao,Yan Zhang,Kaiming Yang,Longlong Luo

Aging cell 19:e13252 PubMed33040455

2020

Elevated miR-124-3p in the aging colon disrupts mucus barrier and increases susceptibility to colitis by targeting T-synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Li Huang,Ting-Yi Sun,Liang-Jun Hu,Shi-Long Hu,Hai-Mei Sun,Fu-Qian Zhao,Bo Wu,Shu Yang,Feng-Qing Ji,De-Shan Zhou

Cancer management and research 12:1017-1027 PubMed32104083

2020

Demethylation of the Cosmc Promoter Alleviates the Progression of Breast Cancer Through Downregulation of the Tn and Sialyl-Tn Antigens.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Xu,Dong Wang,JianXiu Cui,Jie Li,Hongchuan Jiang

Cancer immunology research 8:167-178 PubMed31831633

2019

Cross-talk between Colon Cells and Macrophages Increases ST6GALNAC1 and MUC1-sTn Expression in Ulcerative Colitis and Colitis-Associated Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Kvorjak,Yasmine Ahmed,Michelle L Miller,Raahul Sriram,Claudia Coronnello,Jana G Hashash,Douglas J Hartman,Cheryl A Telmer,Natasa Miskov-Zivanov,Olivera J Finn,Sandra Cascio

Experimental & molecular medicine 50:1-13 PubMed30305605

2018

Loss of complex O-glycosylation impairs exocrine pancreatic function and induces MODY8-like diabetes in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Gerrit Wolters-Eisfeld,Baris Mercanoglu,Bianca T Hofmann,Thomas Wolpers,Claudia Schnabel,Sönke Harder,Pascal Steffen,Kai Bachmann,Babett Steglich,Jörg Schrader,Nicola Gagliani,Hartmut Schlüter,Cenap Güngör,Jakob R Izbicki,Christoph Wagener,Maximilian Bockhorn

mAbs 9:615-627 PubMed28281872

2017

Novel anti-Sialyl-Tn monoclonal antibodies and antibody-drug conjugates demonstrate tumor specificity and anti-tumor activity.

Applications

Unspecified application

Species

Unspecified reactive species

Jillian M Prendergast,Ana Paula Galvao da Silva,David A Eavarone,Darius Ghaderi,Mai Zhang,Dane Brady,Joan Wicks,Julie DeSander,Jeff Behrens,Bo R Rueda

Oncotarget 8:80083-80092 PubMed29108390

2017

High truncated-O-glycan score predicts adverse clinical outcome in patients with localized clear-cell renal cell carcinoma after surgery.

Applications

Unspecified application

Species

Unspecified reactive species

SonTung NguyenHoang,Yidong Liu,Le Xu,Lin Zhou,Yuan Chang,Qiang Fu,Zheng Liu,Zongming Lin,Jiejie Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sialyl Tn antibody [STn 219]

Flow Cytometry - Anti-Sialyl Tn antibody [STn 219] (AB115957)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (10)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise