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AB233146

Anti-SIGIRR antibody

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(3 Publications)

Rabbit Polyclonal SIGIRR antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SIGIRR aa 1-150.

View Alternative Names

UNQ301/PRO342, SIGIRR, Single Ig IL-1-related receptor, Single Ig IL-1R-related molecule, Single immunoglobulin domain-containing IL1R-related protein, Toll/interleukin-1 receptor 8, TIR8

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGIRR antibody (AB233146)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGIRR antibody (AB233146)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for SIGIRR using ab233146 at 30 μg/ml in immunohistochemical analysis.

Secondary used HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGIRR antibody (AB233146)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGIRR antibody (AB233146)

Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for SIGIRR using ab233146 at 30 μg/ml in immunohistochemical analysis.

Secondary used HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGIRR antibody (AB233146)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGIRR antibody (AB233146)

Formalin-fixed, paraffin-embedded human liver tissue stained for SIGIRR using ab233146 at 30 μg/ml in immunohistochemical analysis.

Secondary used HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGIRR antibody (AB233146)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGIRR antibody (AB233146)

Formalin-fixed, paraffin-embedded human prostate tissue stained for SIGIRR using ab233146 at 30 μg/ml in immunohistochemical analysis.

Secondary used HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Western blot - Anti-SIGIRR antibody (AB233146)
  • WB

Supplier Data

Western blot - Anti-SIGIRR antibody (AB233146)

All lanes:

Western blot - Anti-SIGIRR antibody (ab233146) at 2 µg/mL

All lanes:

Recombinant human SIGIRR protein

Predicted band size: 122 kDa,18 kDa,46 kDa

false

Western blot - Anti-SIGIRR antibody (AB233146)
  • WB

Supplier Data

Western blot - Anti-SIGIRR antibody (AB233146)

All lanes:

Western blot - Anti-SIGIRR antibody (ab233146) at 2 µg/mL

All lanes:

Pig kidney lysate

Secondary

All lanes:

HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/mL

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SIGIRR aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q6IA17

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIGIRR also known as Single Immunoglobulin IL-1 Related Receptor or TIR8 is a transmembrane protein approximately 410 amino acids in length translating to a mass of about 45 kDa. It is expressed in various tissues with higher levels seen in the kidney liver lung and immune cells. SIGIRR is part of the Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) family acting as a negative regulator by interfering with signaling pathways usually triggered by these receptors.
Biological function summary

SIGIRR modulates immune responses by dampening inflammation and maintaining homeostasis in immune-related pathways. SIGIRR functions as a decoy receptor binding ligands without initiating a signaling cascade which effectively reduces the immune response. It does not work alone; it is part of larger receptor complexes within the TLR and IL-1R family where it can inhibit the signaling of other family members like TLR4 and IL-1R1 thereby controlling their activity and preventing excessive inflammation.

Pathways

SIGIRR participates in both the Toll-like receptor and interleukin-1 receptor pathways. It interacts with adaptor proteins such as MyD88 to modulate the downstream signaling cascade that involves NF-kB activation a central transcription factor in immune response. By doing so it keeps the inflammation response in check connecting to related proteins within these pathways that include IL-1R1 and TLR4 ultimately regulating key pro-inflammatory processes.

Multiple studies link SIGIRR to conditions characterized by chronic inflammation such as inflammatory bowel disease (IBD) and rheumatoid arthritis (RA). Animals with SIGIRR deficiency tend to show increased susceptibility to these diseases implicating its role in disease modulation. Additionally SIGIRR's interaction with IL-1R1 highlights its potential involvement in autoimmune diseases where dysregulated immune regulation is a common theme. Understanding its function in these contexts can provide insights into therapeutic approaches for inflammatory and autoimmune conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. Attenuates the recruitment of receptor-proximal signaling components to the TLR4 receptor, probably through an TIR-TIR domain interaction with TLR4. Through its extracellular domain interferes with the heterodimerization of Il1R1 and IL1RAP.
See full target information SIGIRR
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Asia Pacific allergy 14:97-102 PubMed39220569

2024

Enhanced IL-37-IL-1R8 axis is negatively associated with inflammatory and clinical severity of chronic rhinosinusitis with nasal polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Zhang,Yujie Cao,Kun Chen,Xianting Hu,Chun Zhou,Lei Li,Miaomiao Han,Dehui Wang,Huabin Li

Journal of hepatocellular carcinoma 10:2337-2353 PubMed38163053

2024

Hepatitis B Virus-Encoded MicroRNA (HBV-miR-3) Inhibits FIH-1 Expression to Promote Tumor Angiogenesis in HBV-Related Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Han Chen,Dan Cao,Ning Han,Mingming Zhang,Wei Jiang,Xin Wang,Qinmin Zeng,Hong Tang

Communications biology 3:306 PubMed32533049

2020

Induction of IRAK-M in melanoma induces caspase-3 dependent apoptosis by reducing TRAF6 and calpastatin levels.

Applications

Unspecified application

Species

Unspecified reactive species

Degui Geng,Nicholas Ciavattone,Jackline Joy Lasola,Rojesh Shrestha,Amelia Sanchez,Jitao Guo,Alexandra Vlk,Rania Younis,Lucy Wang,Alex J Brown,Yuji Zhang,Cruz Velasco-Gonzalez,Aik-Choon Tan,Eduardo Davila
View all publications
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Product promise

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