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AB250012

Anti-SIGIRR antibody [EPR12638] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SIGIRR antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

UNQ301/PRO342, SIGIRR, Single Ig IL-1-related receptor, Single Ig IL-1R-related molecule, Single immunoglobulin domain-containing IL1R-related protein, Toll/interleukin-1 receptor 8, TIR8

1 Images
Western blot - Anti-SIGIRR antibody [EPR12638] - BSA and Azide free (AB250012)
  • WB

Supplier Data

Western blot - Anti-SIGIRR antibody [EPR12638] - BSA and Azide free (AB250012)

This data was developed using ab177937, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SIGIRR antibody [EPR12638] (<a href='/en-us/products/primary-antibodies/sigirr-antibody-epr12638-ab177937'>ab177937</a>) at 1/1000 dilution

All lanes:

Fetal kidney lysate at 10 µg

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12638

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGIRR antibody [EPR22438-93] - BSA and Azide free (AB256314)

  • 0
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Complementary Products

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Primary Antibodies

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Primary Antibodies

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Reactivity data

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Product details

ab250012 is the carrier-free version of ab177937.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIGIRR also known as Single Immunoglobulin IL-1 Related Receptor or TIR8 is a transmembrane protein approximately 410 amino acids in length translating to a mass of about 45 kDa. It is expressed in various tissues with higher levels seen in the kidney liver lung and immune cells. SIGIRR is part of the Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) family acting as a negative regulator by interfering with signaling pathways usually triggered by these receptors.
Biological function summary

SIGIRR modulates immune responses by dampening inflammation and maintaining homeostasis in immune-related pathways. SIGIRR functions as a decoy receptor binding ligands without initiating a signaling cascade which effectively reduces the immune response. It does not work alone; it is part of larger receptor complexes within the TLR and IL-1R family where it can inhibit the signaling of other family members like TLR4 and IL-1R1 thereby controlling their activity and preventing excessive inflammation.

Pathways

SIGIRR participates in both the Toll-like receptor and interleukin-1 receptor pathways. It interacts with adaptor proteins such as MyD88 to modulate the downstream signaling cascade that involves NF-kB activation a central transcription factor in immune response. By doing so it keeps the inflammation response in check connecting to related proteins within these pathways that include IL-1R1 and TLR4 ultimately regulating key pro-inflammatory processes.

Multiple studies link SIGIRR to conditions characterized by chronic inflammation such as inflammatory bowel disease (IBD) and rheumatoid arthritis (RA). Animals with SIGIRR deficiency tend to show increased susceptibility to these diseases implicating its role in disease modulation. Additionally SIGIRR's interaction with IL-1R1 highlights its potential involvement in autoimmune diseases where dysregulated immune regulation is a common theme. Understanding its function in these contexts can provide insights into therapeutic approaches for inflammatory and autoimmune conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. Attenuates the recruitment of receptor-proximal signaling components to the TLR4 receptor, probably through an TIR-TIR domain interaction with TLR4. Through its extracellular domain interferes with the heterodimerization of Il1R1 and IL1RAP.
See full target information SIGIRR
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:65 PubMed39972431

2025

Mechanism of histone demethylase KDM5A in osteoporotic fracture healing through epigenetic regulation of the miR-495/SKP2/Runx2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoran Li,Junyan Zhang,Tingting Xu,Zhiying Hao,Yadong Li
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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