JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB267396

Anti-Siglec 7 antibody [EPR23118-130] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Siglec 7 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD328, AIRM1, SIGLEC7, Sialic acid-binding Ig-like lectin 7, Siglec-7, Adhesion inhibitory receptor molecule 1, CDw328, D-siglec, QA79 membrane protein, p75, AIRM-1

2 Images
Flow Cytometry - Anti-Siglec 7 antibody [EPR23118-130] - BSA and Azide free (AB267396)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Siglec 7 antibody [EPR23118-130] - BSA and Azide free (AB267396)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling Siglec 7 with ab243643 at 1\500 (right panel) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left panel). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

Cells were stained with rabbit IgG (Left) or ab243643 (Right). Then stained with anti-CD56 conjugated to BV421.

Gated on viable lymphocytes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243643).

Flow Cytometry - Anti-Siglec 7 antibody [EPR23118-130] - BSA and Azide free (AB267396)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Siglec 7 antibody [EPR23118-130] - BSA and Azide free (AB267396)

Flow cytometric analysis of human monocytes cells labeling Siglec 7 with ab243643 at 1\500 compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable monocytes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243643).

  • Unconjugated

    Anti-Siglec 7 antibody [EPR23118-130]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Siglec 7 antibody [EPR23118-130]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Siglec 7 antibody [EPR23118-130]

  • 578 PE

    PE Anti-Siglec 7 antibody [EPR23118-130]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23118-130

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB111619

Anti-Siglec 7 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Siglec 7 antibody (AB111619)

  • 0
  • 0
Primary Antibodies

AB234144

Anti-Siglec 7 antibody [6-434]

Flow Cytometry - Anti-Siglec 7 antibody [6-434] (AB234144)

  • 0
  • 0
Primary Antibodies

AB200558

PE Anti-Siglec 7 antibody [6-434]

Flow Cytometry - PE Anti-Siglec 7 antibody [6-434] (AB200558)

  • 0
  • 0
Primary Antibodies

AB133388

Anti-Siglec 7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Siglec 7 antibody (AB133388)

  • 0
  • 0

Complementary Products

Assay Kits

AB267615

Human Siglec 7 ELISA Kit

Sandwich ELISA - Human Siglec 7 ELISA Kit (AB267615)

  • 0
  • 0
Primary Antibodies

AB197981

Anti-SIGLEC9 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC9 antibody (AB197981)

  • 0
  • 0
Secondary Antibodies

AB125912

Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L

RabMAb|Recombinant

ELISA - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (AB125912)

  • 0
  • 0
Primary Antibodies

AB243658

Anti-SLAMF6 antibody [EPR23122-130]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR23122-130] (AB243658)

  • 0
  • 0
Primary Antibodies

AB245735

Anti-Sialoadhesin/CD169 antibody [SP213] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - BSA and Azide free (AB245735)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

ab267396 is the carrier-free version of ab243643.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro).
See full target information SIGLEC7
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com