Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (AB307651)

This data was developed using ab307650, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. This antibody does not cross-react with human SIGLEC8, SIGLEC9 and SIGLEC12. Exposure time : 26 seconds.

All lanes:

Western blot - Anti-Siglec 7 antibody [EPR26252-79] (<a href='/en-us/products/primary-antibodies/siglec-7-antibody-epr26252-79-ab307650'>ab307650</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with a human SIGLEC7 expression vector containing a myc-his tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human SIGLEC8 expression vector containing a myc-his tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human SIGLEC9 expression vector containing a myc-his tag whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human SIGLEC12 expression vector containing a myc-his tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 49 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (AB307651)

This data was developed using ab307650, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : skeletal muscle (PMID : 10567377). This blot was developed using a high sensitivity ECL substrate. Exposure time : 180 seconds.

All lanes:

Western blot - Anti-Siglec 7 antibody [EPR26252-79] (<a href='/en-us/products/primary-antibodies/siglec-7-antibody-epr26252-79-ab307650'>ab307650</a>) at 1/1000 dilution

Lane 1:

Mouse placenta tissue lysate at 20 µg

Lane 2:

Rat placenta tissue lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Lane 4:

Human placenta tissue lysate at 20 µg

Lane 5:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (AB307651)

This data was developed using ab307650, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-Siglec 7 antibody [EPR26252-79] (<a href='/en-us/products/primary-antibodies/siglec-7-antibody-epr26252-79-ab307650'>ab307650</a>) at 1/1000 dilution

Lane 1:

U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 2:

RPMI-8226 (human plasmacytoma, myeloma B lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Observed band size: 75 kDa

true

Exposure time: 180s