Rabbit Recombinant Monoclonal Siglec 7 antibody. Carrier free. Suitable for WB and reacts with Rat, Mouse, Human samples.
Images
![Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (AB307651), expandable thumbnail](https://content.abcam.com/products/images/siglec-7-antibody-epr26252-79-bsa-and-azide-free-ab307651--western-blot-img406558.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (AB307651)")
![Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (AB307651), expandable thumbnail](https://content.abcam.com/products/images/siglec-7-antibody-epr26252-79-bsa-and-azide-free-ab307651--western-blot-img406559.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (AB307651)")
![Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (AB307651), expandable thumbnail](https://content.abcam.com/products/images/siglec-7-antibody-epr26252-79-bsa-and-azide-free-ab307651--western-blot-img406557.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (AB307651)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | Flow Cyt | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Target data
Function
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro).
Alternative names
CD328, AIRM1, SIGLEC7, Sialic acid-binding Ig-like lectin 7, Siglec-7, Adhesion inhibitory receptor molecule 1, CDw328, D-siglec, QA79 membrane protein, p75, AIRM-1
Recommended products
Rabbit Recombinant Monoclonal Siglec 7 antibody. Carrier free. Suitable for WB and reacts with Rat, Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26252-79
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (ab307651), expandable thumbnail](https://content.abcam.com/products/images/siglec-7-antibody-epr26252-79-bsa-and-azide-free-ab307651--western-blot-img406558.jpg "Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (ab307651)")
Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (ab307651)
This data was developed using Anti-Siglec 7 antibody [EPR26252-79] ab307650, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-Siglec 7 antibody [EPR26252-79] (Anti-Siglec 7 antibody [EPR26252-79] ab307650) at 1/1000 dilution
Lane 1: U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg
Lane 2: RPMI-8226 (human plasmacytoma, myeloma B lymphocyte) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Developed using the ECL technique.
Observed band size: 75 kDa
Exposure time: 180s
![Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (ab307651), expandable thumbnail](https://content.abcam.com/products/images/siglec-7-antibody-epr26252-79-bsa-and-azide-free-ab307651--western-blot-img406559.jpg "Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (ab307651)")
Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (ab307651)
This data was developed using Anti-Siglec 7 antibody [EPR26252-79] ab307650, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human SIGLEC8, SIGLEC9 and SIGLEC12.
Exposure time: 26 seconds.All lanes: Western blot - Anti-Siglec 7 antibody [EPR26252-79] (Anti-Siglec 7 antibody [EPR26252-79] ab307650) at 1/1000 dilution
Lane 1: 293T cells transfected with a human SIGLEC7 expression vector containing a myc-his tag whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human SIGLEC8 expression vector containing a myc-his tag whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human SIGLEC9 expression vector containing a myc-his tag whole cell lysate at 20 µg
Lane 4: 293T cells transfected with a human SIGLEC12 expression vector containing a myc-his tag whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 49 kDa
Exposure time: 26s
![Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (ab307651), expandable thumbnail](https://content.abcam.com/products/images/siglec-7-antibody-epr26252-79-bsa-and-azide-free-ab307651--western-blot-img406557.jpg "Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (ab307651)")
Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (ab307651)
This data was developed using Anti-Siglec 7 antibody [EPR26252-79] ab307650, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: skeletal muscle (PMID: 10567377).
This blot was developed using a high sensitivity ECL substrate.
Exposure time: 180 seconds.All lanes: Western blot - Anti-Siglec 7 antibody [EPR26252-79] (Anti-Siglec 7 antibody [EPR26252-79] ab307650) at 1/1000 dilution
Lane 1: Mouse placenta tissue lysate at 20 µg
Lane 2: Rat placenta tissue lysate at 20 µg
Lane 3: Human liver tissue lysate at 20 µg
Lane 4: Human placenta tissue lysate at 20 µg
Lane 5: Human skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 75 kDa
Exposure time: 180s
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