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AB106174

Anti-SIGLEC H antibody [440c]

0

(1 Review)

|

(2 Publications)

Rat Monoclonal SIGLEC H antibody. Suitable for Flow Cyt, FuncS (Neut/Block), IHC-Fr and reacts with Mouse samples. Cited in 2 publications.

View Alternative Names

SIGLEC like protein, Siglech, sialic acid binding Ig like lectin H

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

440c

Isotype

IgG2b

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt, FuncS (Neut/Block), IHC-Fr

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p>For inhibition of IFN-alpha secretion dilutions have to be made according to the amounts of SIGLEC H to be inactivated.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

Incubation of interferon producing cells with antibody 440c in vitro or administration of antibody 440c in vivo reduces secretion of IFN-alpha in response to CpG DNA without causing interferon producing cells depletion.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab106174 was 0.2 µm filtered prior to formulation.
Storage buffer
Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIGLEC H also known as sialic acid-binding Ig-like lectin H weighs about 50 kDa. It is found on the surface of plasmacytoid dendritic cells (pDCs) and it is closely related to the immune system. This protein lacks a signaling domain but it can pair with other proteins to transmit signals across cell membranes. The expression of SIGLEC H is highly specific to pDCs making it a useful marker for these cells in various immunological studies.
Biological function summary

This protein plays an important role in the immune response. SIGLEC H functions in the recognition and uptake of pathogens through endocytosis by binding sialylated glycoproteins. Unlike some other members of the SIGLEC family it is not part of a complex and relies on interactions with other signaling molecules. These interactions facilitate phagocytosis and antigen presentation which are critical functions of pDCs in adaptive immunity.

Pathways

SIGLEC H becomes involved in the innate immune signaling pathways that regulate type I interferon production. The pathways include the Toll-like receptor (TLR) signaling pathway. SIGLEC H modulates TLR7 and TLR9 pathways in pDCs which manage the production of cytokines and chemokines. It can also influence the expression of interferon regulatory factors further impacting cytokine production and modulating immune response.

The improper function or regulation of SIGLEC H affects autoimmune diseases like systemic lupus erythematosus. Anomalies in SIGLEC H might also have implications for viral infections due to its role in pathogen detection and immune responses. Proteins such as TLR7 and interferon regulatory factor 7 (IRF7) show connections with SIGLEC H within these contexts often determining the outcome of immune-mediated and infectious diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SIGLEC H
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Blood 107:2474-6 PubMed16293595

2005

Siglec-H is an IPC-specific receptor that modulates type I IFN secretion through DAP12.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda L Blasius,Marina Cella,Jorge Maldonado,Toshiyuki Takai,Marco Colonna

Blood 103:4201-6 PubMed14695235

2003

A cell-surface molecule selectively expressed on murine natural interferon-producing cells that blocks secretion of interferon-alpha.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda Blasius,William Vermi,Anne Krug,Fabio Facchetti,Marina Cella,Marco Colonna
View all publications
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