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AB249295

Anti-SIGLEC11 antibody [EPR8506(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal SIGLEC11 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

UNQ9222/PRO28718, SIGLEC11, Sialic acid-binding Ig-like lectin 11, Sialic acid-binding lectin 11, Siglec-11

1 Images
Western blot - Anti-SIGLEC11 antibody [EPR8506(2)] - BSA and Azide free (AB249295)
  • WB

Unknown

Western blot - Anti-SIGLEC11 antibody [EPR8506(2)] - BSA and Azide free (AB249295)

This data was developed using ab157187, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SIGLEC11 antibody [EPR8506(2)] - BSA and Azide free (ab249295) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8506(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249295 is the carrier-free version of ab157187.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIGLEC11 also known as sialic acid-binding Ig-like lectin 11 is an inhibitory receptor protein characterized by its ability to bind sialic acids. It has a mass of approximately 66 kDa. This protein contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that helps transmit inhibitory signals. SIGLEC11 is predominantly expressed in the immune cells including macrophages and dendritic cells within the central nervous system (CNS). It is also found in certain peripheral tissues.
Biological function summary

SIGLEC11 plays a role in modulating immune responses and maintaining immune homeostasis. By binding to sialic acid-containing glycoproteins SIGLEC11 can influence cell-cell interactions and prevent unnecessary activation of immune cells. It does not form part of a larger complex but directly participates by recruiting phosphatases such as SHP-1 or SHP-2. These recruitments help dephosphorylate substrates in turn dampening immune cell activation.

Pathways

SIGLEC11 contributes to the negative regulation of receptor signaling pathways relevant to the immune system. Within the signaling pathways it impacts the downstream signaling cascade that involves Src family kinases. SIGLEC11 shares interactions with other members of the SIGLEC family engaging in pathways that modulate cellular activation and inflammation such as the MAPK pathway. This ensures balanced immune responses to avoid excessive inflammation.

SIGLEC11 expressions show relevance to neurodegenerative diseases and autoimmune disorders. Altered SIGLEC11 activity is associated with Alzheimer's disease where it may dampen the inflammatory response within the CNS providing a protective mechanism. In autoimmune conditions such as systemic lupus erythematosus (SLE) SIGLEC11 may interact with proteins that regulate self-tolerance and immune activation mitigating inappropriate immune responses. Understanding its function helps to reveal insights into disease mechanisms and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,8-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules.
See full target information SIGLEC11
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com