Rabbit Recombinant Monoclonal SIGLEC5 antibody. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (AB307434), expandable thumbnail](https://content.abcam.com/products/images/siglec5-antibody-epr26250-156-ab307434--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405673.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (AB307434)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (AB307434), expandable thumbnail](https://content.abcam.com/products/images/siglec5-antibody-epr26250-156-ab307434--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405672.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (AB307434)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (AB307434), expandable thumbnail](https://content.abcam.com/products/images/siglec5-antibody-epr26250-156-ab307434--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405671.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (AB307434)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (AB307434), expandable thumbnail](https://content.abcam.com/products/images/siglec5-antibody-epr26250-156-ab307434--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405670.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (AB307434)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell line - Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse, Transfected cell line - Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse, Transfected cell line - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human, Transfected cell line - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Transfected cell line - Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human, Transfected cell line - Human | Dilution info - | Notes - |
Target data
Function
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Alternative names
CD170, CD33L2, OBBP2, SIGLEC5, Sialic acid-binding Ig-like lectin 5, Siglec-5, CD33 antigen-like 2, Obesity-binding protein 2, OB-BP2, OB-binding protein 2
Recommended products
Rabbit Recombinant Monoclonal SIGLEC5 antibody. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR26250-156
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SIGLEC5 also known as CD170 is a sialic acid-binding Ig-like lectin with a molecular mass of approximately 96 kDa. This protein plays an important role in immune cell signaling by recognizing sialylated glycans on the surface of cells. SIGLEC5 is mainly expressed on immune cells such as neutrophils monocytes and macrophages. It belongs to the lectin family of proteins and can modulate immune responses and communication between cells.
- Biological function summary
SIGLEC5 functions by mediating cell-cell interactions and transmitting inhibitory signals that regulate immune responses. It binds to sialic acid-containing glycoproteins which can help modulate the threshold for cell activation by transmitting signals through its immunoreceptor tyrosine-based inhibitory motif (ITIM). SIGLEC5 is not part of a large complex but interacts with other molecules to exert its function in maintaining immune cell balance.
- Pathways
SIGLEC5 plays a role in immune signaling pathways by maintaining cell homeostasis. It contributes to the regulation of the immune response by modulating inhibitory signaling pathways. It is associated with proteins like Src family kinases which phosphorylate the ITIM motif to initiate downstream signaling cascades. Additionally its interaction with sialic acids allows modulation of the innate immune response to various stimuli.
- Associated diseases and disorders
SIGLEC5 has been linked to autoimmune diseases and infections like bacterial sepsis. In autoimmune conditions the dysregulation of SIGLEC5-associated pathways can lead to improper immune cell activation and tissue damage. In the context of bacterial sepsis SIGLEC5 assists in dampening excessive immune responses and limiting inflammation. SIGLEC5 connects with proteins like DAP12 where aberrations could also influence disease progressions making it a target of interest for potential therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (ab307434), expandable thumbnail](https://content.abcam.com/products/images/siglec5-antibody-epr26250-156-ab307434--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405673.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (ab307434)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (ab307434)
Immunohistochemical analysis of paraffin-embedded Panel A HEK-293T c tissue lABeling SIGLEC5 with ab307434 at 1/2000 (0.26 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Cytoplasmic staining on (A) HEK-293T transfected with a SIGLEC5 expression vector and (B) HEK-293T transfected with a SIGLEC14 expression vector, no staining on (C) HEK-293T transfected with empty vector. The section was incubated with ab307434 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (ab307434), expandable thumbnail](https://content.abcam.com/products/images/siglec5-antibody-epr26250-156-ab307434--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405672.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (ab307434)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (ab307434)
Immunohistochemical analysis of paraffin-embedded Human stomach tissue lABeling SIGLEC5 with ab307434 at 1/2000 (0.26 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on scattered immune cells of human stomach. The section was incubated with ab307434 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (ab307434), expandable thumbnail](https://content.abcam.com/products/images/siglec5-antibody-epr26250-156-ab307434--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405671.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (ab307434)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (ab307434)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue lABeling SIGLEC5 with ab307434 at 1/2000 (0.26 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on human spleen. The section was incubated with ab307434 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (ab307434), expandable thumbnail](https://content.abcam.com/products/images/siglec5-antibody-epr26250-156-ab307434--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405670.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (ab307434)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [EPR26250-156] (ab307434)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue lABeling SIGLEC5 with ab307434 at 1/2000 (0.26 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control: no staining on human kidney. The section was incubated with ab307434 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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