Anti-SIGLEC5 antibody [RM2001]

8 product images

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  Flow Cytometry (Intracellular) - Anti-SIGLEC5 antibody [RM2001] (ab307846)

  Flow cytometric analysis of HEK-293T (human embryonic kidney) cells transfected with human SIGLEC14 (Left) or SIGLEC5 (Right) expression vector containing GFP tag cells labeling SIGLEC5 and SIGLEC14 with ab307846 at 1/500 dilution (0.1µg). Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.
  Cross-react with SIGLEC14.
  Gated on viable cells.

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  Flow Cytometry (Intracellular) - Anti-SIGLEC5 antibody [RM2001] (ab307846)

  Flow cytometric analysis of K-562(human chronic myelogenous leukemia lymphoblast) (Left panel) compared with U-937
  (human histiocytic lymphoma monocyte) (Right panel) labeling SIGLEC5 with ab307846 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
  Negative control: K-562.
  Gated on viable cells.

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  Immunocytochemistry/ Immunofluorescence - Anti-SIGLEC5 antibody [RM2001] (ab307846)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labeling SIGLEC5 and SIGLEC14 with ab307846 at 1/50 dilution (9.72 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088) antibody at 1/1000 dilution (2 µg/ml) (Green).
  Confocal image showing positive staining in HEK-293T cells transfected with a human SIGLEC14 or SIGLEC5 expression vector containing a myc tag.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Nuclear counterstain was DAPI (Blue).
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088) at 1/1000 dilution (2 µg/ml).

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  Immunocytochemistry/ Immunofluorescence - Anti-SIGLEC5 antibody [RM2001] (ab307846)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-937 (human histiocytic lymphoma monocyte) cells labeling SIGLEC5 with ab307846 at 1/50 dilution (9.72 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green).
  Confocal image showing membranous and cytoplasmic staining in U-937 cell line.
  Negative control: K-562 (PMID: 9731071).
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). The nuclear counterstain was DAPI (Blue).
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [RM2001] (ab307846)

  Immunohistochemical analysis of paraffin-embedded cell pellets labeling SIGLEC5 with ab307846 at 1/4000 dilution (0.122 µg/ml) followed by ready to use LeicaDS9800 (Bond ® Polymer Refine Detection).
  Positive staining on (A) HEK-293T transfected with a SIGLEC5 expression vector and (B) HEK-293T transfected with a SIGLEC14 expression vector, no staining on (C) HEK-293T transfected with empty vector.
  The section was incubated with ab307846 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [RM2001] (ab307846)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling SIGLEC5 with ab307846 at 1/500 dilution (0.972 µg/ml) followed by ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
  Negative control: No staining on human skeletal muscle (PMID: 9731071).
  The section was incubated with ab307846 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [RM2001] (ab307846)

  Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue labeling SIGLEC5 with ab307846 at 1/500 dilution (0.972 µg/ml) followed by ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
  Positive staining on human stomach carcinoma. 
  The section was incubated with ab307846 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC5 antibody [RM2001] (ab307846)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling SIGLEC5 with ab307846 at 1/500 dilution (0.972 µg/ml) followed by ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
  Positive staining on human spleen. 
  The section was incubated with ab307846 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.