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Rabbit Recombinant Monoclonal SIGLEC6 antibody. Suitable for mIHC, WB, IHC-P and reacts with Human samples.

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Images

Multiplex immunohistochemistry - Anti-SIGLEC6 antibody [EPR28608-317] (AB317307), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-SIGLEC6 antibody [EPR28608-317] (AB317307), expandable thumbnail
  • Western blot - Anti-SIGLEC6 antibody [EPR28608-317] (AB317307), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC6 antibody [EPR28608-317] (AB317307), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC6 antibody [EPR28608-317] (AB317307), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCWBIHC-PFlow CytIPICC/IF
Human
Tested
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Alternative names

CD327, CD33L, CD33L1, OBBP1, SIGLEC6, Sialic acid-binding Ig-like lectin 6, Siglec-6, CD33 antigen-like 1, CDw327, Obesity-binding protein 1, OB-BP1

Recommended products

Rabbit Recombinant Monoclonal SIGLEC6 antibody. Suitable for mIHC, WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28608-317
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SIGLEC6 also known as CD327 is a protein found on some immune cells particularly on B cells and to a lesser extent on macrophages and dendritic cells. This protein has a molecular mass of around 100 kDa. As a member of the sialic acid-binding immunoglobulin-type lectins family (siglecs) SIGLEC6 mechanistically functions by recognizing and binding to specific sialic acid-containing carbohydrates on cell surfaces. This recognition influences cell-cell interactions and signaling processes important for immune response modulation.

Biological function summary

Four words that are not SIGLEC6 illustrate its involvement in immune system regulation. SIGLEC6 serves as an inhibitory receptor that helps dampen B cell receptor signaling balancing the immune response to prevent overactivation. It often partners with other proteins in complex cellular interactions within the immune cells. The expression of SIGLEC6 in placental tissues also suggests a role in trophoblast invasion and development although this function requires more research to fully understand the mechanisms involved.

Pathways

Four words that are not SIGLEC6 describe its integration into the immune signaling pathways particularly the immune response regulation. SIGLEC6 is a part of the B cell receptor signaling pathway acting as an inhibitory modulator. Through this pathway it connects with proteins like CD22 and other siglecs that contribute to the regulation of B cell activity. Additionally its role in glycan recognition links it to pathways associated with cell surface interactions and immune tolerance.

Associated diseases and disorders

Four words that are not SIGLEC6 highlight its relevance to certain conditions. SIGLEC6 expression alterations have implications in chronic lymphocytic leukemia (CLL) where it potentially influences disease progression by modulating B cell signaling. The protein has also been connected to gestational trophoblastic diseases potentially affecting placental development. In these contexts SIGLEC6 interacts with proteins such as CD22 in CLL where they collectively affect leukemic cell behaviors and disease outcomes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Multiplex immunohistochemistry - Anti-SIGLEC6 antibody [EPR28608-317] (ab317307), expandable thumbnail

    Multiplex immunohistochemistry - Anti-SIGLEC6 antibody [EPR28608-317] (ab317307)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human lung tissue staining Fc epsilon RI/FCER1A with Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] ab316199 at a 1:500 (1.04 ug/ml) dilution and SIGLEC6 with ab317307 at 1:100 (4.89 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

    Panel A: merged staining of anti-Fc epsilon RI/FCER1A (green; Opal™520) and anti-SIGLEC6 (magenta; Opal™690) on human lung.
    Panel B: anti-Fc epsilon RI/FCER1A staining immune cells in human lung.
    Panel C: anti-SIGLEC6 staining mast cells and basophils in human lung.
    Panel D: Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in two rounds of staining: in the order of Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] ab316199 and ab317307 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Multiplex immunohistochemistry - Anti-SIGLEC6 antibody [EPR28608-317] (ab317307), expandable thumbnail

    Multiplex immunohistochemistry - Anti-SIGLEC6 antibody [EPR28608-317] (ab317307)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human stomach cancer tissue staining Fc epsilon RI/FCER1A with Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] ab316199 at a 1:500 (1.04 ug/ml) dilution and SIGLEC6 with ab317307 at 1:100 (4.89 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

    Panel A: merged staining of anti-Fc epsilon RI/FCER1A (green; Opal™520) and anti-SIGLEC6 (magenta; Opal™690) on human stomach cancer.
    Panel B: anti-Fc epsilon RI/FCER1A staining immune cells in human stomach cancer.
    Panel C: anti-SIGLEC6 staining mast cells and basophils in human stomach cancer.
    Panel D: Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in two rounds of staining: in the order of Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] ab316199 and ab317307 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-SIGLEC6 antibody [EPR28608-317] (ab317307), expandable thumbnail

    Western blot - Anti-SIGLEC6 antibody [EPR28608-317] (ab317307)

    Negative control: hypothalamus, brain, kidney, liver (PMID: 17580316; PMID:33633313).

    The molecular weight observed is consistent with what has been described in the literature (PMID: 32998819).

    Samples are non-boiled as boiling may cause protein aggregates.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-SIGLEC6 antibody [EPR28608-317] (ab317307) at 1/1000 dilution

    Lane 1: Human placenta tissue lysate at 20 µg with NFDM/TBST

    Lane 2: Human hypothalamus tissue lysate at 20 µg with NFDM/TBST

    Lane 3: Human brain tissue lysate at 20 µg with NFDM/TBST

    Lane 4: Human kidney tissue lysate at 20 µg with NFDM/TBST

    Lane 5: Human liver tissue lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 70 kDa, 36 kDa

    Exposure time: 48s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC6 antibody [EPR28608-317] (ab317307), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC6 antibody [EPR28608-317] (ab317307)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling SIGLEC6 with ab317307 at 1/100 (4.89 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human cerebrum (PMID: 17580316).

    The section was incubated with ab317307 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC6 antibody [EPR28608-317] (ab317307), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC6 antibody [EPR28608-317] (ab317307)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SIGLEC6 with ab317307 at 1/100 (4.89 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on immune cells of human colon (PMID: 30294327).

    The section was incubated with ab317307 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC6 antibody [EPR28608-317] (ab317307), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC6 antibody [EPR28608-317] (ab317307)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling SIGLEC6 with ab317307 at 1/100 (4.89 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human placenta (PMID: 17580316, 23089140).

    The section was incubated with ab317307 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

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