Skip to main content

Rabbit Recombinant Monoclonal SIGLEC6 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Sandwich ELISA - Anti-SIGLEC6 antibody [EPR28608-321] - BSA and Azide free (Detector) (AB321831), expandable thumbnail
  • Sandwich ELISA - Anti-SIGLEC6 antibody [EPR28608-321] - BSA and Azide free (Detector) (AB321831), expandable thumbnail
  • Sandwich ELISA - Anti-SIGLEC6 antibody [EPR28608-321] - BSA and Azide free (Detector) (AB321831), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Alternative names

Sialic acid-binding Ig-like lectin 6, Siglec-6, CD33 antigen-like 1, CDw327, Obesity-binding protein 1, OB-BP1, SIGLEC6, CD33L, CD33L1, OBBP1

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Rabbit Recombinant Monoclonal SIGLEC6 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Alternative names

Sialic acid-binding Ig-like lectin 6, Siglec-6, CD33 antigen-like 1, CDw327, Obesity-binding protein 1, OB-BP1, SIGLEC6, CD33L, CD33L1, OBBP1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR28608-321

Purification technique

Affinity purification Protein A

Specificity

This antibody has 66% cross reaction with human SIGLEC7, but does not cross-react with human SIGLEC2, SIGLEC3, SIGLEC5, SIGLEC8, SIGLEC9, SIGLEC10, SIGLEC11, SIGLEC12 and SIGLEC14.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab321831 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human SIGLEC6. The recommended pair for sandwich ELISA is:

Capture: Anti-SIGLEC6 antibody [EPR28608-331] - BSA and Azide free (Capture) ab321830, Human SIGLEC6 Capture Antibody (unconjugated)

Detector: ab321831, Human SIGLEC6 Detector Antibody (unconjugated)

The reference range value is 31.25-2000 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

Activity summary

SIGLEC6 also known as CD327 is a protein found on some immune cells particularly on B cells and to a lesser extent on macrophages and dendritic cells. This protein has a molecular mass of around 100 kDa. As a member of the sialic acid-binding immunoglobulin-type lectins family (siglecs) SIGLEC6 mechanistically functions by recognizing and binding to specific sialic acid-containing carbohydrates on cell surfaces. This recognition influences cell-cell interactions and signaling processes important for immune response modulation.

Biological function summary

Four words that are not SIGLEC6 illustrate its involvement in immune system regulation. SIGLEC6 serves as an inhibitory receptor that helps dampen B cell receptor signaling balancing the immune response to prevent overactivation. It often partners with other proteins in complex cellular interactions within the immune cells. The expression of SIGLEC6 in placental tissues also suggests a role in trophoblast invasion and development although this function requires more research to fully understand the mechanisms involved.

Pathways

Four words that are not SIGLEC6 describe its integration into the immune signaling pathways particularly the immune response regulation. SIGLEC6 is a part of the B cell receptor signaling pathway acting as an inhibitory modulator. Through this pathway it connects with proteins like CD22 and other siglecs that contribute to the regulation of B cell activity. Additionally its role in glycan recognition links it to pathways associated with cell surface interactions and immune tolerance.

Associated diseases and disorders

Four words that are not SIGLEC6 highlight its relevance to certain conditions. SIGLEC6 expression alterations have implications in chronic lymphocytic leukemia (CLL) where it potentially influences disease progression by modulating B cell signaling. The protein has also been connected to gestational trophoblastic diseases potentially affecting placental development. In these contexts SIGLEC6 interacts with proteins such as CD22 in CLL where they collectively affect leukemic cell behaviors and disease outcomes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Anti-SIGLEC6 antibody [EPR28608-321] - BSA and Azide free (Detector) (ab321831), expandable thumbnail

    Sandwich ELISA - Anti-SIGLEC6 antibody [EPR28608-321] - BSA and Azide free (Detector) (ab321831)

    Interpolated concentration of native SIGLEC6 was measured at different sample concentrations. Undiluted sample is as follows: U-937 cell extract (100 ug/mL). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-SIGLEC6 antibody [EPR28608-321] - BSA and Azide free (Detector) (ab321831), expandable thumbnail

    Sandwich ELISA - Anti-SIGLEC6 antibody [EPR28608-321] - BSA and Azide free (Detector) (ab321831)

    Interpolated concentration of native SIGLEC6 was measured at different sample concentrations. Undiluted samples are as follows: human serum 100% and human plasma (EDTA) 50%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-SIGLEC6 antibody [EPR28608-321] - BSA and Azide free (Detector) (ab321831), expandable thumbnail

    Sandwich ELISA - Anti-SIGLEC6 antibody [EPR28608-321] - BSA and Azide free (Detector) (ab321831)

    Sandwich ELISA of Human SIGLEC6 Antibody Pair - BSA and Azide free ab321829 with the capture antibody Anti-SIGLEC6 antibody [EPR28608-331] - BSA and Azide free (Capture) ab321830 dilution at 2 ug/mL and detector antibody ab321831 dilution at 0.5 ug/mL.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com