Rabbit Recombinant Monoclonal SIGLEC8 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Transfected cell lysate - Human, Human, Transfected cell line - Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (AB305298), expandable thumbnail](https://content.abcam.com/products/images/siglec8-antibody-epr24419-56-bsa-and-azide-free-ab305298--flow-cytometry-intracellular-img403938.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (AB305298)")
![Immunocytochemistry/ Immunofluorescence - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (AB305298), expandable thumbnail](https://content.abcam.com/products/images/siglec8-antibody-epr24419-56-bsa-and-azide-free-ab305298--immunocytochemistry-immunofluorescence-img403911.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (AB305298)")
![Immunoprecipitation - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (AB305298), expandable thumbnail](https://content.abcam.com/products/images/siglec8-antibody-epr24419-56-bsa-and-azide-free-ab305298--immunoprecipitation-img404438.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (AB305298)")
![Western blot - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (AB305298), expandable thumbnail](https://content.abcam.com/products/images/siglec8-antibody-epr24419-56-bsa-and-azide-free-ab305298--western-blot-img404133.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (AB305298)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Human | Expected | Not recommended | Tested | Expected | Expected |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell line - Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Transfected cell lysate - Human | Tested | Not recommended | Not recommended | Not recommended | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Transfected cell lysate - Human | Dilution info - | Notes - |
Species Transfected cell line - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Putative adhesion molecule that mediates sialic-acid dependent binding to blood cells (PubMed:10625619, PubMed:10856141). Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface (PubMed:10625619). Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes (PubMed:27357658).
Alternative names
SAF2, SIGLEC8, Sialic acid-binding Ig-like lectin 8, Siglec-8, Sialoadhesin family member 2, SAF-2
Recommended products
Rabbit Recombinant Monoclonal SIGLEC8 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Transfected cell lysate - Human, Human, Transfected cell line - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24419-56
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SIGLEC8 also known as Sialic acid-binding Ig-like lectin 8 is a protein that functions primarily as a receptor. It has a molecular mass of approximately 50 kDa. SIGLEC8 is expressed on the surface of certain immune cells specifically eosinophils basophils and to a lesser extent mast cells. This receptor plays an important role in the immune responses especially those involving inflammatory processes due to its ability to recognize and bind sialylated glycan ligands.
- Biological function summary
SIGLEC8 serves as an inhibitory receptor on these immune cells which can help regulate their activation and survival. It does not form part of a large protein complex but its action can affect various cellular functions such as cell apoptosis and signaling processes. By binding to its specific glycan ligands SIGLEC8 reduces the lifespan of eosinophils which are often involved in allergic responses and asthma thereby helping to control inflammatory responses in tissues.
- Pathways
SIGLEC8 is involved in pathways that regulate the immune system particularly those controlling eosinophil apoptosis and inflammation. It interacts with signaling pathways that include the Src family kinases which are central to signal transduction in immune cells. Additionally it shares functional relationships with other receptors in the SIGLEC family which modulate immune responses through similar mechanisms.
- Associated diseases and disorders
SIGLEC8 is significantly associated with asthma and other allergic diseases. The protein's ability to induce apoptosis in eosinophils makes it an important target for therapies aimed at controlling eosinophil-mediated inflammation in asthma. It is also related to the IL-5 signaling pathway as IL-5 plays a role in the survival and activation of eosinophils. By influencing these pathways SIGLEC8 holds potential as a therapeutic target for reducing symptoms and exacerbations in allergic conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry (Intracellular) - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (ab305298), expandable thumbnail](https://content.abcam.com/products/images/siglec8-antibody-epr24419-56-bsa-and-azide-free-ab305298--flow-cytometry-intracellular-img403938.jpg "Flow Cytometry (Intracellular) - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (ab305298)")
Flow Cytometry (Intracellular) - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (ab305298)
This data was developed using Anti-SIGLEC8 antibody [EPR24419-56] ab305297, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized HEK-293T (human embryonic kidney) cells transfected with a human SIGLEC8 expression vector containing a myc-his tag cells labelling SIGLEC8 with Anti-SIGLEC8 antibody [EPR24419-56] ab305297 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Live cells were surface stained with Anti-SIGLEC8 antibody [EPR24419-56] ab305297, then fixed with 2% PFA followed by intracellular staining with Myc-tag conjugated to Alexa Fluor® 647.
![Immunocytochemistry/ Immunofluorescence - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (ab305298), expandable thumbnail](https://content.abcam.com/products/images/siglec8-antibody-epr24419-56-bsa-and-azide-free-ab305298--immunocytochemistry-immunofluorescence-img403911.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (ab305298)")
Immunocytochemistry/ Immunofluorescence - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (ab305298)
This data was developed using Anti-SIGLEC8 antibody [EPR24419-56] ab305297, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling SIGLEC8 with Anti-SIGLEC8 antibody [EPR24419-56] ab305297 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HEK-293T cells transfected with a human SIGLEC8 expression vector containing a myc tag. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red).
The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
![Immunoprecipitation - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (ab305298), expandable thumbnail](https://content.abcam.com/products/images/siglec8-antibody-epr24419-56-bsa-and-azide-free-ab305298--immunoprecipitation-img404438.jpg "Immunoprecipitation - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (ab305298)")
Immunoprecipitation - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (ab305298)
This data was developed using Anti-SIGLEC8 antibody [EPR24419-56] ab305297, the same antibody clone in a different buffer formulation.
SIGLEC8 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) cells transfected with a human SIGLEC8 expression vector containing a myc-His-tag®, whole cell lysate, 2 μg with Anti-SIGLEC8 antibody [EPR24419-56] ab305297 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-SIGLEC8 antibody [EPR24419-56] ab305297 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HEK-293T (human embryonic kidney epithelial cell) cells transfected with a human SIGLEC8 expression vector containing a myc-His-tag®, whole cell lysate, 2 μg
Lane 2: Anti-SIGLEC8 antibody [EPR24419-56] ab305297 IP in HEK-293T cells transfected with a human SIGLEC8 expression vector containing a myc-His-tag®, whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SIGLEC8 antibody [EPR24419-56] ab305297 in HEK-293T cells transfected with a human SIGLEC8 expression vector containing a myc-His-tag®, whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 secondsAll lanes: Immunoprecipitation - Anti-SIGLEC8 antibody [EPR24419-56] (Anti-SIGLEC8 antibody [EPR24419-56] ab305297) at 1/30 dilution
All lanes: HEK-293T (human embryonic kidney epithelial cell)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 60 kDa
Exposure time: 3s
![Western blot - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (ab305298), expandable thumbnail](https://content.abcam.com/products/images/siglec8-antibody-epr24419-56-bsa-and-azide-free-ab305298--western-blot-img404133.jpg "Western blot - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (ab305298)")
Western blot - Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free (ab305298)
This data was developed using Anti-SIGLEC8 antibody [EPR24419-56] ab305297, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 1 secondAll lanes: Western blot - Anti-SIGLEC8 antibody [EPR24419-56] (Anti-SIGLEC8 antibody [EPR24419-56] ab305297) at 1/1000 dilution
Lane 1: HEK-293T cells transfected with a human SIGLEC8 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: HEK-293T transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 60 kDa
Exposure time: 1s
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