AB151288

Anti-Sigma1-receptor antibody

KO Validated
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(4 Publications)

Rabbit Polyclonal Sigma1-receptor antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SIGMAR1 aa 1 to C-terminus.

View Alternative Names

OPRS1, SRBP, AAG8, SIGMAR1, Sigma non-opioid intracellular receptor 1, Aging-associated gene 8 protein, SR31747-binding protein, Sigma 1-type opioid receptor, SR-BP, SIG-1R, Sigma1-receptor, Sigma1R, hSigmaR1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody (AB151288)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody (AB151288)

Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft labeling Sigma1-receptor with ab151288 at 1/100 dilution.

Lab

Western blot - Anti-Sigma1-receptor antibody (AB151288)

Lanes 1 - 4 : Merged signal (red and green). Green - ab151288 observed at 24 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab151288 was shown to react with Sigma1-receptor in wild-type HEK-293T cells in Western blot with loss of signal observed in SIGMAR1 knockout cell line ab266619 (SIGMAR1 knockout cell lysate ab258666). Wild-type HEK-293T and SIGMAR1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab151288 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

Lanes 1 - 4:

Western blot - Anti-Sigma1-receptor antibody (ab151288) at 1/500 dilution

Lanes 1 - 4:

Western blot - Anti-Sigma1-receptor antibody (<a href='/en-us/products/primary-antibodies/sigma1-receptor-antibody-ab223702'>ab223702</a>) at 0.04 µg/mL

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

SIGMAR1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human SIGMAR1 (Sigma1-receptor) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-sigmar1-sigma1-receptor-knockout-hek-293t-cell-line-ab266619'>ab266619</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Caco-2 cell lysate at 20 µg

Predicted band size: 25 kDa

Observed band size: 24 kDa

false

Lab

Western blot - Anti-Sigma1-receptor antibody (AB151288)

Lanes 1 - 4 : Merged signal (red and green). Green - ab151288 observed at 24 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

All lanes:

Western blot - Anti-Sigma1-receptor antibody (ab151288) at 1/500 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

SIGMAR1 knockout HEK-293T cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Caco-2 cell lysate at 20 µg

Predicted band size: 25 kDa

Observed band size: 24 kDa

false

Supplier Data

Western blot - Anti-Sigma1-receptor antibody (AB151288)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Sigma1-receptor antibody (ab151288) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 25 kDa

false

Supplier Data

Western blot - Anti-Sigma1-receptor antibody (AB151288)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Sigma1-receptor antibody (ab151288) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysates at 50 µg

Lane 2:

Rat liver tissue lysates at 50 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 25 kDa

false

Supplier Data

Western blot - Anti-Sigma1-receptor antibody (AB151288)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Sigma1-receptor antibody (ab151288) at 1/1000 dilution

Lane 1:

Wild-type (WT) HeLa cell extracts at 30 µg

Lane 2:

SIGMAR1 knockout (KO) HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SIGMAR1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99720

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions in lipid transport from the endoplasmic reticulum and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. Involved in the regulation of different receptors it plays a role in BDNF signaling and EGF signaling. Also regulates ion channels like the potassium channel and could modulate neurotransmitter release. Plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. Plays a role in several other cell functions including proliferation, survival and death. Originally identified for its ability to bind various psychoactive drugs it is involved in learning processes, memory and mood alteration (PubMed : 16472803, PubMed : 9341151). Necessary for proper mitochondrial axonal transport in motor neurons, in particular the retrograde movement of mitochondria. Plays a role in protecting cells against oxidative stress-induced cell death via its interaction with RNF112 (By similarity).

See full target information SIGMAR1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Circulation journal : official journal of the Japanese Circulation Society 85:2102-2108 PubMed34176868

2021

Visualization of Dynamic Expression of Myocardial Sigma-1 Receptor After Myocardial Ischemia and Reperfusion Using Radioiodine-Labeled 2-[4-(2-iodophenyl)piperidino]cyclopentanol (OI5V) Imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroshi Wakabayashi,Junichi Taki,Hiroshi Mori,Tomo Hiromasa,Norihito Akatani,Anri Inaki,Takashi Kozaka,Kazuhiro Shiba,Kazuma Ogawa,Seigo Kinuya

Diabetes, metabolic syndrome and obesity : targets 13:1417-1428 PubMed32431525

2020

Swimming Differentially Affects T2DM-Induced Skeletal Muscle ER Stress and Mitochondrial Dysfunction Related to MAM.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Zhang,Di Cui,Tan Zhang,Yi Sun,Shuzhe Ding

Aging cell 18:e13037 PubMed31560162

2019

Lifelong choline supplementation ameliorates Alzheimer's disease pathology and associated cognitive deficits by attenuating microglia activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ramon Velazquez,Eric Ferreira,Sara Knowles,Chaya Fux,Alexis Rodin,Wendy Winslow,Salvatore Oddo

Cancer medicine 6:819-833 PubMed28332309

2017

Effects of miR-145-5p through NRAS on the cell proliferation, apoptosis, migration, and invasion in melanoma by inhibiting MAPK and PI3K/AKT pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Liu,Guozhen Gao,Dexiong Yan,Xiangjun Chen,Xingwei Yao,Shuzhong Guo,Guirong Li,Yu Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sigma1-receptor antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody (AB151288)

Western blot - Anti-Sigma1-receptor antibody (AB151288)

Western blot - Anti-Sigma1-receptor antibody (AB151288)

Western blot - Anti-Sigma1-receptor antibody (AB151288)

Western blot - Anti-Sigma1-receptor antibody (AB151288)

Western blot - Anti-Sigma1-receptor antibody (AB151288)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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