Anti-Sigma1-receptor antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody (AB223702)

Immunohistochemical analysis of Formalin fixed, Paraffin-embedded human Fallopian tube tissue labeling Sigma 1-receptor with ab223702 at 1/20 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody (AB223702)

Immunohistochemical analysis of Formalin fixed, Paraffin-embedded human gastrointestinal tissue labeling Sigma 1-receptor with ab223702 at 1/20 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody (AB223702)

Immunohistochemical analysis of Formalin fixed, Paraffin-embedded human cerebellum tissue labeling Sigma 1-receptor with ab223702 at 1/20 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody (AB223702)

Immunohistochemical analysis of Formalin fixed, Paraffin-embedded human liver tissue labeling Sigma 1-receptor with ab223702 at 1/20 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Sigma1-receptor antibody (AB223702)

Lanes 1 - 4 : Merged signal (red and green). Green - ab223702 observed at 24 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab223702 was shown to react with Sigma1-receptor in wild-type HEK-293T cells in Western blot with loss of signal observed in SIGMAR1 knockout cell line ab266619 (SIGMAR1 knockout cell lysate ab258666). Wild-type HEK-293T and SIGMAR1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab223702 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at 0.04 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

Lanes 1 - 4:

Western blot - Anti-Sigma1-receptor antibody (<a href='/en-us/products/primary-antibodies/sigma1-receptor-antibody-ab151288'>ab151288</a>) at 1/500 dilution

Lanes 1 - 4:

Western blot - Anti-Sigma1-receptor antibody (ab223702) at 0.04 µg/mL

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

SIGMAR1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human SIGMAR1 (Sigma1-receptor) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-sigmar1-sigma1-receptor-knockout-hek-293t-cell-line-ab266619'>ab266619</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Caco-2 cell lysate at 20 µg

Predicted band size: 25 kDa

Observed band size: 24 kDa

false

• WB

Supplier Data

Western blot - Anti-Sigma1-receptor antibody (AB223702)

All lanes:

Western blot - Anti-Sigma1-receptor antibody (ab223702) at 1/100 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 25 kDa

false