Rabbit Polyclonal Sigma1-receptor antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SIGMAR1 aa 1-100.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SIGMAR1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q99720
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Guinea pig, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Guinea pig, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Functions in lipid transport from the endoplasmic reticulum and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. Involved in the regulation of different receptors it plays a role in BDNF signaling and EGF signaling. Also regulates ion channels like the potassium channel and could modulate neurotransmitter release. Plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. Plays a role in several other cell functions including proliferation, survival and death. Originally identified for its ability to bind various psychoactive drugs it is involved in learning processes, memory and mood alteration (PubMed:16472803, PubMed:9341151). Necessary for proper mitochondrial axonal transport in motor neurons, in particular the retrograde movement of mitochondria. Plays a role in protecting cells against oxidative stress-induced cell death via its interaction with RNF112 (By similarity).
Alternative names
OPRS1, SRBP, AAG8, SIGMAR1, Sigma non-opioid intracellular receptor 1, Aging-associated gene 8 protein, SR31747-binding protein, Sigma 1-type opioid receptor, SR-BP, SIG-1R, Sigma1-receptor, Sigma1R, hSigmaR1
Recommended products
Rabbit Polyclonal Sigma1-receptor antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SIGMAR1 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SIGMAR1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q99720
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody (ab223702)
Immunohistochemical analysis of Formalin fixed, Paraffin-embedded human liver tissue labeling Sigma 1-receptor with ab223702 at 1/20 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody (ab223702)
Immunohistochemical analysis of Formalin fixed, Paraffin-embedded human cerebellum tissue labeling Sigma 1-receptor with ab223702 at 1/20 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Sigma1-receptor antibody (ab223702)
Lanes 1 - 4: Merged signal (red and green). Green - ab223702 observed at 24 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.ab223702 was shown to react with Sigma1-receptor in wild-type HEK-293T cells in Western blot with loss of signal observed in SIGMAR1 knockout cell line Human SIGMAR1 (Sigma1-receptor) knockout HEK-293T cell line ab266619 (SIGMAR1 knockout cell lysate Human SIGMAR1 (Sigma1-receptor) knockout HEK-293T cell lysate ab258666). Wild-type HEK-293T and SIGMAR1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab223702 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at 0.04 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Lanes 1 - 4: Western blot - Anti-Sigma1-receptor antibody (Anti-Sigma1-receptor antibody ab151288) at 1/500 dilution
Lanes 1 - 4: Western blot - Anti-Sigma1-receptor antibody (ab223702) at 0.04 µg/mL
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: SIGMAR1 knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human SIGMAR1 (Sigma1-receptor) knockout HEK-293T cell line (Human SIGMAR1 (Sigma1-receptor) knockout HEK-293T cell line ab266619)
Lane 3: HeLa cell lysate at 20 µg
Lane 4: Caco-2 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 24 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody (ab223702)
Immunohistochemical analysis of Formalin fixed, Paraffin-embedded human Fallopian tube tissue labeling Sigma 1-receptor with ab223702 at 1/20 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody (ab223702)
Immunohistochemical analysis of Formalin fixed, Paraffin-embedded human gastrointestinal tissue labeling Sigma 1-receptor with ab223702 at 1/20 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Sigma1-receptor antibody (ab223702)
All lanes: Western blot - Anti-Sigma1-receptor antibody (ab223702) at 1/100 dilution
All lanes: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 25 kDa
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