Anti-Sigma1-receptor antibody [EPR25145-11] - BSA and Azide free

10 product images

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  Western blot - Anti-Sigma1-receptor antibody [EPR25145-11] - BSA and Azide free (ab307549)

  This data was developed using 307548, the same antibody clone in a different buffer formulation.
  Blocking and diluting buffer and concentration: Lysates at 20 ?g per lane.
  
  Performed under reducing conditions.
  
  In Western blot, Anti-Sigma1-receptor antibody [EPR25145-11] ab307548 was shown to bind specifically to SIGMAR1. A band was observed at 26 kDa in wild-type 293T cell lysates with whereas no signal observed at this size in SIGMAR1 knockout cell line Human SIGMAR1 (Sigma1-receptor) knockout HEK-293T cell line ab266619 (knockout cell lysate Human SIGMAR1 (Sigma1-receptor) knockout HEK-293T cell lysate ab258666).
  Lanes 1-2: 180 seconds;
  Lane 3: 136 seconds.
  Exposure time:

  All lanes: Western blot - Anti-Sigma1-receptor antibody [EPR25145-11] (Anti-Sigma1-receptor antibody [EPR25145-11] ab307548) at 1/1000 dilution

  Lane 1: Wild-type HEK293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg

  Lane 2: SIGMAR1 knockout HEK293T whole cell lysate 20 μg

  Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg

  Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20 μg

  Lane 5: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 20 μg

  Lane 6: C6 (rat glial tumor glial cell) whole cell lysate 20 μg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Performed under reducing conditions.

  Observed band size: 26 kDa

  Exposure time: 180s

• 

  Western blot - Anti-Sigma1-receptor antibody [EPR25145-11] - BSA and Azide free (ab307549)

  This data was developed using 307548, the same antibody clone in a different buffer formulation.
  Blocking and diluting buffer and concentration: Low expression: brain (PMID: 28350844), spleen (PMID: 28350844).
  
  Exposure time:

  All lanes: Western blot - Anti-Sigma1-receptor antibody [EPR25145-11] (Anti-Sigma1-receptor antibody [EPR25145-11] ab307548) at 1/1000 dilution

  Lane 1: Mouse liver tissue lysate 20 μg

  Lane 2: Mouse brain tissue lysate 20 μg

  Lane 3: Rat liver tissue lysate 20 μg

  Lane 4: Rat brain tissue lysate 20 μg

  Lane 5: Human liver tissue lysate 20 μg

  Lane 6: Human spleen tissue lysate 20 μg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 26 kDa

  Exposure time: 8s

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody [EPR25145-11] - BSA and Azide free (ab307549)

  This data was developed using Anti-Sigma1-receptor antibody [EPR25145-11] ab307548, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Sigma1-receptor with Anti-Sigma1-receptor antibody [EPR25145-11] ab307548 at 1/1000 (0.533 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on rat liver.The section was incubated with Anti-Sigma1-receptor antibody [EPR25145-11] ab307548 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody [EPR25145-11] - BSA and Azide free (ab307549)

  This data was developed using Anti-Sigma1-receptor antibody [EPR25145-11] ab307548, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Sigma1-receptor with Anti-Sigma1-receptor antibody [EPR25145-11] ab307548 at 1/1000 (0.533 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on mouse cerebrum (PMID: 31596232).The section was incubated with Anti-Sigma1-receptor antibody [EPR25145-11] ab307548 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody [EPR25145-11] - BSA and Azide free (ab307549)

  This data was developed using Anti-Sigma1-receptor antibody [EPR25145-11] ab307548, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Sigma1-receptor with Anti-Sigma1-receptor antibody [EPR25145-11] ab307548 at 1/1000 (0.533 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on mouse liver.The section was incubated with Anti-Sigma1-receptor antibody [EPR25145-11] ab307548 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody [EPR25145-11] - BSA and Azide free (ab307549)

  This data was developed using Anti-Sigma1-receptor antibody [EPR25145-11] ab307548, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Sigma1-receptor with Anti-Sigma1-receptor antibody [EPR25145-11] ab307548 at 1/1000 (0.533 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on human liver (PMID: 29399701).The section was incubated with Anti-Sigma1-receptor antibody [EPR25145-11] ab307548 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody [EPR25145-11] - BSA and Azide free (ab307549)

  This data was developed using Anti-Sigma1-receptor antibody [EPR25145-11] ab307548, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded (A) Wild-type HEK-29 tissue labeling Sigma1-receptor with Anti-Sigma1-receptor antibody [EPR25145-11] ab307548 at 1/1000 (0.533 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on (A) Wild-type HEK-293T cell pellet, no staining on (B) SIGMAR1 knockout HEK-293T (Human SIGMAR1 (Sigma1-receptor) knockout HEK-293T cell line ab266619) cell pellet.The section was incubated with Anti-Sigma1-receptor antibody [EPR25145-11] ab307548 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Flow Cytometry (Intracellular) - Anti-Sigma1-receptor antibody [EPR25145-11] - BSA and Azide free (ab307549)

  This data was developed using Anti-Sigma1-receptor antibody [EPR25145-11] ab307548, the same antibody clone in a different buffer formulation.
  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeABilized Wild-type 293T (human embryonic kidney epithelial cell, Right) / Sigma1-receptor knockout 293T (Left) cells lABelling Sigma1-receptor with Anti-Sigma1-receptor antibody [EPR25145-11] ab307548 at 1/500 dilution (0.1ug) (Red) (Red) compared with a RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlABelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-RABbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Positive staining on 293T cells (ab255449), while no staining on Sigma1-receptor knockout 293T cells (Human SIGMAR1 (Sigma1-receptor) knockout HEK-293T cell line ab266619).

• 

  Flow Cytometry (Intracellular) - Anti-Sigma1-receptor antibody [EPR25145-11] - BSA and Azide free (ab307549)

  This data was developed using Anti-Sigma1-receptor antibody [EPR25145-11] ab307548, the same antibody clone in a different buffer formulation.
  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeABilized NIH/3T3 (mouse embryonic fibroblast) cells lABelling Sigma1-receptor with Anti-Sigma1-receptor antibody [EPR25145-11] ab307548 at 1/50 dilution (1ug) (Red) (Red) compared with a RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlABelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-RABbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

• 

  Flow Cytometry (Intracellular) - Anti-Sigma1-receptor antibody [EPR25145-11] - BSA and Azide free (ab307549)

  This data was developed using Anti-Sigma1-receptor antibody [EPR25145-11] ab307548, the same antibody clone in a different buffer formulation.
  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeABilized C6 (rat glial tumor glial cell) cells lABelling Sigma1-receptor with Anti-Sigma1-receptor antibody [EPR25145-11] ab307548 at 1/50 dilution (1ug) (Red) (Red) compared with a RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlABelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-RABbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.