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AB271963

Anti-SIK1 (pT182) + SIK2 (pT175) + SIK3 (pT163) antibody [EPR19196] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SIK2 phospho T175 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide - Human, Transfected cell lysate - Human samples. Cited in 1 publication.

View Alternative Names

KIAA0781, QIK, SNF1LK2, SIK2, Serine/threonine-protein kinase SIK2, Qin-induced kinase, Salt-inducible kinase 2, Serine/threonine-protein kinase SNF1-like kinase 2, SIK-2

1 Images
Dot Blot - Anti-SIK1 (pT182) + SIK2 (pT175) + SIK3 (pT163) antibody [EPR19196] - BSA and Azide free (AB271963)
  • Dot

Lab

Dot Blot - Anti-SIK1 (pT182) + SIK2 (pT175) + SIK3 (pT163) antibody [EPR19196] - BSA and Azide free (AB271963)

Dot blot analysis of Snf1lk2 (phospho T175) labeled with ab199474 at 1/1,000 dilution.

Lane 1 : Snf1lk2 (phospho T175) peptide

Lane 2 : Snf1lk2 non-phospho peptide

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100,000 dilution was used as secondary antibody.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199474).

  • Unconjugated

    Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19196

Isotype

IgG

Carrier free

Yes

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Expected to react with Snf1lk (when phosphorylated at T182) and SIK3 ((when phosphorylated at T163).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab271963 is the carrier-free version of ab199474.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIK1 SIK2 and SIK3 also known as salt-inducible kinases are serine/threonine protein kinases that play a role in cellular signaling. These proteins have molecular weights of approximately 75 kDa 105 kDa and 145 kDa respectively. SIK1 SIK2 and SIK3 share structural similarities but differ in their tissue expression. SIK1 is mostly found in the heart and liver SIK2 in adipose tissue and SIK3 shows widespread expression including the brain and skeletal muscle. Mechanically these kinases modify other proteins through phosphorylation influencing cellular activity.
Biological function summary

These kinases regulate various processes essential for maintaining cellular homeostasis. They are not part of any well-known complex but interact with several cellular components. SIKs influence gene expression by inhibiting transcriptional regulators and also regulate metabolism and cell survival. Their action impacts how cells respond to external stimuli largely through modification of transcriptional activity in response to hormonal signals and nutrient availability.

Pathways

SIK1 SIK2 and SIK3 are integral to the AMP-activated protein kinase (AMPK) signaling pathway and the cAMP response element-binding protein (CREB) pathway. These pathways control energy balance and respond to stress conditions. SIKs act alongside AMPK to modulate cellular energy status and also influence the CREB signaling pathway by impacting phosphorylation state. Regulatory relationships exist with proteins like 14-3-3 and HDACs which themselves are key players in cellular metabolism and gene expression.

These kinases have links to metabolic diseases and cancer. SIKs have been associated with obesity due to their role in adipocyte differentiation and lipid metabolism. They also relate to pancreatic cancer where altered kinase activity disrupts normal cell function and proliferation. In cancer SIKs interact with proteins involved in proliferation pathways including mTOR affecting tumor growth and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase that plays a role in many biological processes such as fatty acid oxidation, autophagy, immune response or glucose metabolism (PubMed : 23322770, PubMed : 26983400). Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators (PubMed : 15454081). Phosphorylates EP300 and thus inhibits its histone acetyltransferase activity (PubMed : 21084751, PubMed : 26983400). In turn, regulates the DNA-binding ability of several transcription factors such as PPARA or MLXIPL (PubMed : 21084751, PubMed : 26983400). Also plays a role in thymic T-cell development (By similarity).
See full target information SIK2 phospho T175

Additional targets

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genetics 225: PubMed37477881

2023

Sleep need, the key regulator of sleep homeostasis, is indicated and controlled by phosphorylation of threonine 221 in salt-inducible kinase 3.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Chengang Li,Yuxiang Liu,Jianjun Yu,Jingqun Yang,Yunfeng Cui,Tao V Wang,Chaoyi Li,Lifen Jiang,Meilin Song,Yi Rao
View all publications
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