AB53423

Anti-SIK2 antibody

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(6 Publications)

Rabbit Polyclonal SIK2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human SIK2 aa 100-150.

View Alternative Names

KIAA0781, QIK, SNF1LK2, SIK2, Serine/threonine-protein kinase SIK2, Qin-induced kinase, Salt-inducible kinase 2, Serine/threonine-protein kinase SNF1-like kinase 2, SIK-2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK2 antibody (AB53423)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK2 antibody (AB53423)

IHC image of ab53423 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53423, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Western blot - Anti-SIK2 antibody (AB53423)

NB While the predicted molecular weight is 104kDa, both the bands at 120kDa and at 25kDa are blocked by immunizing peptide.

All lanes:

Western blot - Anti-SIK2 antibody (ab53423) at 2 µg/mL

Lane 1:

Human brain

Lane 2:

Human brain with immunizing peptide

Predicted band size: 104 kDa

Observed band size: 120 kDa,25 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SIK2 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q9H0K1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIK2 also known as Salt-Inducible Kinase 2 is a protein kinase involved in various cellular processes. It has a molecular mass of approximately 94 kDa. SIK2 is expressed in many tissues with high levels found in adipose tissue liver and skeletal muscle. As a kinase it phosphorylates specific target proteins regulating their activity and influencing cellular mechanisms.

Biological function summary

SIK2 participates in energy metabolism and cellular response to external stress. It interacts with several co-factors to form active complexes facilitating its activity. This protein regulates lipid and carbohydrate metabolism therefore playing a significant role in maintaining cellular energy homeostasis. Additionally SIK2 has a function in controlling the inflammatory response providing a link between metabolism and immune function.

Pathways

SIK2 acts as an essential mediator in the AMPK signaling pathway. This pathway is important for energy balance within cells and is related to other kinases like AMPK and other SIK family members. SIK2 also contributes to the regulation of the cAMP signaling pathway which is linked to various physiological processes. Through these pathways SIK2 influences processes such as glucose uptake and fat oxidation and interacts with proteins like CREB and HDACs.

SIK2 has implications in conditions like obesity and type 2 diabetes. Dysregulation of SIK2 activity can lead to metabolic imbalance and insulin resistance. This kinase is also associated with cancer progression particularly in ovarian cancer where it influences cell proliferation. In these diseases SIK2's connection to proteins such as AMPK and CREB suggests a complex role in disease modulation and potential therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase that plays a role in many biological processes such as fatty acid oxidation, autophagy, immune response or glucose metabolism (PubMed : 23322770, PubMed : 26983400). Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators (PubMed : 15454081). Phosphorylates EP300 and thus inhibits its histone acetyltransferase activity (PubMed : 21084751, PubMed : 26983400). In turn, regulates the DNA-binding ability of several transcription factors such as PPARA or MLXIPL (PubMed : 21084751, PubMed : 26983400). Also plays a role in thymic T-cell development (By similarity).

See full target information SIK2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70341 PubMed40135564

2025

Inhibition of Salt-Inducible Kinase 2 Protects Motor Neurons From Degeneration in ALS by Activating Autophagic Flux and Enhancing mTORC1 Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Liang,Chunting Zhang,Di Wang,Xiaoli Su,Xingli Tan,Yueqing Yang,Chaohua Cong,Ying Wang,Di Huo,Hongyong Wang,Shuyu Wang,Xudong Wang,Honglin Feng

Clinical and translational medicine 12:e890 PubMed35758323

2022

Phosphoproteome reveals molecular mechanisms of aberrant rhythm in neurotransmitter-mediated islet hormone secretion in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yunqiang He,Qi Fu,Min Sun,Yu Qian,Yucheng Liang,Jie Zhang,Rui Gao,Hemin Jiang,Hao Dai,Yuwei Liu,Xinyu Xu,Heng Chen,Kuanfeng Xu,Tao Yang

The Journal of biological chemistry 298:101929 PubMed35413286

2022

Biochemical purification uncovers mammalian sterile 3 (MST3) as a new protein kinase for multifunctional protein kinases AMPK and SIK3.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxiang Liu,Tao V Wang,Yunfeng Cui,Shengxian Gao,Yi Rao

Molecular oncology 16:2558-2574 PubMed35278271

2022

SIK2 promotes ovarian cancer cell motility and metastasis by phosphorylating MYLK.

Applications

Unspecified application

Species

Unspecified reactive species

Xiu Shi,Xuejiao Yu,Juan Wang,Shimin Bian,Qiutong Li,Fengqing Fu,Xinwei Zou,Lin Zhang,Robert C Bast,Zhen Lu,Lingchuan Guo,Youguo Chen,Jinhua Zhou

Journal of translational medicine 19:333 PubMed34353336

2021

MYBL2-induced PITPNA-AS1 upregulates SIK2 to exert oncogenic function in triple-negative breast cancer through miR-520d-5p and DDX54.

Applications

Unspecified application

Species

Unspecified reactive species

Bolong Liu,Pingbo Yao,Feng Xiao,Jianjin Guo,Lianghui Wu,Yong Yang

Toxicological sciences : an official journal of th 132:458-66 PubMed23358194

2013

Transcriptome alterations following developmental atrazine exposure in zebrafish are associated with disruption of neuroendocrine and reproductive system function, cell cycle, and carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Gregory J Weber,Maria S Sepúlveda,Samuel M Peterson,Solange S Lewis,Jennifer L Freeman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SIK2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK2 antibody (AB53423)

Western blot - Anti-SIK2 antibody (AB53423)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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