AB280575

Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SIK2 antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Mouse, Mouse samples.

View Alternative Names

Snf1lk2, Sik2, Serine/threonine-protein kinase SIK2, Salt-inducible kinase 2, Serine/threonine-protein kinase SNF1-like kinase 2, SIK-2

3 Images

Lab

Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (AB280575)

This data was developed using ab245211, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : Lanes 1-2 : 59 seconds; Lane 3 : 3 minutes.

All lanes:

Western blot - Anti-SIK2 antibody [EPR22722-23] (<a href='/en-us/products/primary-antibodies/sik2-antibody-epr22722-23-ab245211'>ab245211</a>) at 1/1000 dilution

Lane 1:

Mouse P70 brown adipose tissue lysate at 20 µg

Lane 2:

Mouse P70 white adipose tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 104 kDa

false

Lab

Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (AB280575)

This data was developed using ab245211, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile observed is consistent with what has been described in the literature (PMID : 12624099)

Exposure time : 3 minutes

All lanes:

Western blot - Anti-SIK2 antibody [EPR22722-23] (<a href='/en-us/products/primary-antibodies/sik2-antibody-epr22722-23-ab245211'>ab245211</a>) at 1/1000 dilution

Lane 1:

Untreated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

3T3-L1 differentiation for 6 days whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 104 kDa

false

Lab

Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (AB280575)

This data was developed using ab245211, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This antibody doesn't react with SIK1.

Exposure time : 3 seconds

All lanes:

Western blot - Anti-SIK2 antibody [EPR22722-23] (<a href='/en-us/products/primary-antibodies/sik2-antibody-epr22722-23-ab245211'>ab245211</a>) at 1/1000 dilution

Lane 1:

293T transfected with a DDDDK-tagged mouse SIK1(WT) expression vector, whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney) transfected with an empty vector (vector control) containing a DDDDK tag, whole cell lysate at 20 µg

Lane 3:

293T transfected with a DDDDK-tagged mouse SIK2(WT) expression vector, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 104 kDa

false

Unconjugated

Anti-SIK2 antibody [EPR22722-23]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22722-23

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab280575 is the carrier-free version of ab245211.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIK2 also known as Salt-Inducible Kinase 2 is a protein kinase involved in various cellular processes. It has a molecular mass of approximately 94 kDa. SIK2 is expressed in many tissues with high levels found in adipose tissue liver and skeletal muscle. As a kinase it phosphorylates specific target proteins regulating their activity and influencing cellular mechanisms.

Biological function summary

SIK2 participates in energy metabolism and cellular response to external stress. It interacts with several co-factors to form active complexes facilitating its activity. This protein regulates lipid and carbohydrate metabolism therefore playing a significant role in maintaining cellular energy homeostasis. Additionally SIK2 has a function in controlling the inflammatory response providing a link between metabolism and immune function.

Pathways

SIK2 acts as an essential mediator in the AMPK signaling pathway. This pathway is important for energy balance within cells and is related to other kinases like AMPK and other SIK family members. SIK2 also contributes to the regulation of the cAMP signaling pathway which is linked to various physiological processes. Through these pathways SIK2 influences processes such as glucose uptake and fat oxidation and interacts with proteins like CREB and HDACs.

SIK2 has implications in conditions like obesity and type 2 diabetes. Dysregulation of SIK2 activity can lead to metabolic imbalance and insulin resistance. This kinase is also associated with cancer progression particularly in ovarian cancer where it influences cell proliferation. In these diseases SIK2's connection to proteins such as AMPK and CREB suggests a complex role in disease modulation and potential therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase that plays a role in many biological processes such as fatty acid oxidation, autophagy, immune response or glucose metabolism (PubMed : 12624099, PubMed : 16817901, PubMed : 29211348). Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction (PubMed : 12624099). Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators (PubMed : 16817901). Phosphorylates EP300 and thus inhibits its histone acetyltransferase activity. In turn, regulates the DNA-binding ability of several transcription factors such as PPARA or MLXIPL (By similarity). Also plays a role in thymic T-cell development (PubMed : 34732767).

See full target information Sik2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free

Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (AB280575)

Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (AB280575)

Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (AB280575)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise