Rabbit Recombinant Monoclonal SIK2 antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Mouse, Mouse samples.
Images
![Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (AB280575), expandable thumbnail](https://content.abcam.com/products/images/sik2-antibody-epr22722-23-bsa-and-azide-free-ab280575--western-blot-img36004.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (AB280575)")
![Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (AB280575), expandable thumbnail](https://content.abcam.com/products/images/sik2-antibody-epr22722-23-bsa-and-azide-free-ab280575--western-blot-img7025.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (AB280575)")
![Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (AB280575), expandable thumbnail](https://content.abcam.com/products/images/sik2-antibody-epr22722-23-bsa-and-azide-free-ab280575--western-blot-img8403.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (AB280575)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | IHC-Fr | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Recombinant fragment - Mouse, Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Recombinant fragment - Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Recombinant fragment - Mouse | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant fragment - Mouse, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Recombinant fragment - Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
Serine/threonine-protein kinase that plays a role in many biological processes such as fatty acid oxidation, autophagy, immune response or glucose metabolism (PubMed:12624099, PubMed:16817901, PubMed:29211348). Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction (PubMed:12624099). Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators (PubMed:16817901). Phosphorylates EP300 and thus inhibits its histone acetyltransferase activity. In turn, regulates the DNA-binding ability of several transcription factors such as PPARA or MLXIPL (By similarity). Also plays a role in thymic T-cell development (PubMed:34732767).
Alternative names
Snf1lk2, Sik2, Serine/threonine-protein kinase SIK2, Salt-inducible kinase 2, Serine/threonine-protein kinase SNF1-like kinase 2, SIK-2
Recommended products
Rabbit Recombinant Monoclonal SIK2 antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Mouse, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22722-23
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab280575 is the carrier-free version of Anti-SIK2 antibody [EPR22722-23] ab245211.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SIK2 also known as Salt-Inducible Kinase 2 is a protein kinase involved in various cellular processes. It has a molecular mass of approximately 94 kDa. SIK2 is expressed in many tissues with high levels found in adipose tissue liver and skeletal muscle. As a kinase it phosphorylates specific target proteins regulating their activity and influencing cellular mechanisms.
- Biological function summary
SIK2 participates in energy metabolism and cellular response to external stress. It interacts with several co-factors to form active complexes facilitating its activity. This protein regulates lipid and carbohydrate metabolism therefore playing a significant role in maintaining cellular energy homeostasis. Additionally SIK2 has a function in controlling the inflammatory response providing a link between metabolism and immune function.
- Pathways
SIK2 acts as an essential mediator in the AMPK signaling pathway. This pathway is important for energy balance within cells and is related to other kinases like AMPK and other SIK family members. SIK2 also contributes to the regulation of the cAMP signaling pathway which is linked to various physiological processes. Through these pathways SIK2 influences processes such as glucose uptake and fat oxidation and interacts with proteins like CREB and HDACs.
- Associated diseases and disorders
SIK2 has implications in conditions like obesity and type 2 diabetes. Dysregulation of SIK2 activity can lead to metabolic imbalance and insulin resistance. This kinase is also associated with cancer progression particularly in ovarian cancer where it influences cell proliferation. In these diseases SIK2's connection to proteins such as AMPK and CREB suggests a complex role in disease modulation and potential therapeutic targeting.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575), expandable thumbnail](https://content.abcam.com/products/images/sik2-antibody-epr22722-23-bsa-and-azide-free-ab280575--western-blot-img36004.jpg "Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575)")
Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575)
This data was developed using Anti-SIK2 antibody [EPR22722-23] ab245211, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile observed is consistent with what has been described in the literature (PMID: 12624099)
Exposure time: 3 minutes
All lanes: Western blot - Anti-SIK2 antibody [EPR22722-23] (Anti-SIK2 antibody [EPR22722-23] ab245211) at 1/1000 dilution
Lane 1: Untreated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 2: 3T3-L1 differentiation for 6 days whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 104 kDa
![Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575), expandable thumbnail](https://content.abcam.com/products/images/sik2-antibody-epr22722-23-bsa-and-azide-free-ab280575--western-blot-img7025.jpg "Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575)")
Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575)
This data was developed using Anti-SIK2 antibody [EPR22722-23] ab245211, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lanes 1-2: 59 seconds; Lane 3: 3 minutes.
All lanes: Western blot - Anti-SIK2 antibody [EPR22722-23] (Anti-SIK2 antibody [EPR22722-23] ab245211) at 1/1000 dilution
Lane 1: Mouse P70 brown adipose tissue lysate at 20 µg
Lane 2: Mouse P70 white adipose tissue lysate at 20 µg
Lane 3: Mouse brain tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 104 kDa
![Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575), expandable thumbnail](https://content.abcam.com/products/images/sik2-antibody-epr22722-23-bsa-and-azide-free-ab280575--western-blot-img8403.jpg "Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575)")
Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575)
This data was developed using Anti-SIK2 antibody [EPR22722-23] ab245211, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody doesn't react with SIK1.
Exposure time: 3 seconds
All lanes: Western blot - Anti-SIK2 antibody [EPR22722-23] (Anti-SIK2 antibody [EPR22722-23] ab245211) at 1/1000 dilution
Lane 1: 293T transfected with a DDDDK-tagged mouse SIK1(WT) expression vector, whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney) transfected with an empty vector (vector control) containing a DDDDK tag, whole cell lysate at 20 µg
Lane 3: 293T transfected with a DDDDK-tagged mouse SIK2(WT) expression vector, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 104 kDa
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