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AB254568

Anti-SIK3 antibody

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(1 Publication)

Chicken Polyclonal SIK3 antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SIK3 aa 1150-1250.

View Alternative Names

KIAA0999, QSK, L19, SIK3, Serine/threonine-protein kinase SIK3, Salt-inducible kinase 3, Serine/threonine-protein kinase QSK, SIK-3

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SIK3 antibody (AB254568)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SIK3 antibody (AB254568)

Immunocytochemistry/immunofluorescent analysis of rat brain cells labeling SIK3 with ab254568 at 20μg/ml.

Western blot - Anti-SIK3 antibody (AB254568)
  • WB

Supplier Data

Western blot - Anti-SIK3 antibody (AB254568)

All lanes:

Western blot - Anti-SIK3 antibody (ab254568) at 1 µg/mL

Lane 1:

Rat brain tissue lysate

Lane 2:

Rat brain tissue lysate with immunizing peptide

Predicted band size: 140 kDa

false

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

No

Reacts with

Rat

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human SIK3 aa 1150-1250. The exact immunogen used to generate this antibody is proprietary information.

Q9Y2K2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIK3 also known as salt-inducible kinase 3 is a serine/threonine-protein kinase with a molecular mass of approximately 140 kDa. This protein is expressed in many tissues including liver muscle and pancreas. Mechanically SIK3 phosphorylates key substrates involved in the regulation of various cellular functions enhancing or inhibiting their activity. Its action often involves regulating metabolism cytokine signaling and cellular growth processes.
Biological function summary

SIK3 participates in the regulation of glucose metabolism and energy homeostasis. It acts as part of larger complexes that control transcription factors and enzymes related to metabolic processes. These complexes help respond to cellular signals and external stress adjusting cellular functions to maintain balance. It influences the activity of enzymes and transcriptional regulators involved in lipid and carbohydrate metabolism playing a role in energy balance maintenance.

Pathways

SIK3 influences gluconeogenesis and insulin signaling pathways. It is closely connected with proteins such as AMPK (AMP-activated protein kinase) regulating energy sensor mechanisms and metabolic stress responses. These pathways are critical in controlling glucose production and utilization which affect overall energy homeostasis in the body.

SIK3's dysfunction links to metabolic disorders like type 2 diabetes and obesity. Altered SIK3 activity affects insulin signaling which can contribute to impaired glucose regulation. It also connects to the protein LKB1 (liver kinase B1) in metabolic disease contexts as both play roles in regulating energy metabolism and maintaining glucose levels. Understanding SIK3's role provides insights for targeting metabolic diseases through therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor. Involved in the dynamic regulation of mTOR signaling in chondrocyte differentiation during skeletogenesis (PubMed : 30232230). Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3 (Probable). Prevents HDAC4 translocation to the nucleus (By similarity).
See full target information SIK3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 12: PubMed33535184

2021

Evaluation of the prognostic values of solute carrier (SLC) family 39 genes for patients with lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Zhou,Yaoqi Zhu,Huizhong Qi,Liang Liang,Hao Wu,Jingping Yuan,Qingyong Hu
View all publications
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Product promise

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