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Chicken Polyclonal SIK3 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SIK3.

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Images

Key facts

Isotype

IgY

Host species

Chicken

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human SIK3. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y2K2
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Expected
Expected
Mouse
Predicted
Predicted
Predicted
Rat
Expected
Tested
Tested

Tested
Tested

Species

Human

Dilution info

5 µg/mL

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1 µg/mL

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

20 µg/mL

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Target data

Function

Positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor. Involved in the dynamic regulation of mTOR signaling in chondrocyte differentiation during skeletogenesis (PubMed:30232230). Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3 (Probable). Prevents HDAC4 translocation to the nucleus (By similarity).

Alternative names

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Chicken Polyclonal SIK3 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SIK3.

Alternative names

Key facts

Isotype

IgY

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human SIK3. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y2K2
Purification technique

Affinity purification Immunogen

Specificity

At least three isoforms of SIK3 are known to exist and ab211424 will detect two isoforms. ab211424 is predicted to not cross-react with SIK1 and SIK2.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

SIK3 participates in the regulation of glucose metabolism and energy homeostasis. It acts as part of larger complexes that control transcription factors and enzymes related to metabolic processes. These complexes help respond to cellular signals and external stress adjusting cellular functions to maintain balance. It influences the activity of enzymes and transcriptional regulators involved in lipid and carbohydrate metabolism playing a role in energy balance maintenance.

Activity summary

SIK3 also known as salt-inducible kinase 3 is a serine/threonine-protein kinase with a molecular mass of approximately 140 kDa. This protein is expressed in many tissues including liver muscle and pancreas. Mechanically SIK3 phosphorylates key substrates involved in the regulation of various cellular functions enhancing or inhibiting their activity. Its action often involves regulating metabolism cytokine signaling and cellular growth processes.

Pathways

SIK3 influences gluconeogenesis and insulin signaling pathways. It is closely connected with proteins such as AMPK (AMP-activated protein kinase) regulating energy sensor mechanisms and metabolic stress responses. These pathways are critical in controlling glucose production and utilization which affect overall energy homeostasis in the body.

Associated diseases and disorders

SIK3's dysfunction links to metabolic disorders like type 2 diabetes and obesity. Altered SIK3 activity affects insulin signaling which can contribute to impaired glucose regulation. It also connects to the protein LKB1 (liver kinase B1) in metabolic disease contexts as both play roles in regulating energy metabolism and maintaining glucose levels. Understanding SIK3's role provides insights for targeting metabolic diseases through therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-SIK3 antibody - C-terminal (ab211424), expandable thumbnail

    Western blot - Anti-SIK3 antibody - C-terminal (ab211424)

    All lanes: Western blot - Anti-SIK3 antibody - C-terminal (AB211424) at 1 µg/mL

    All lanes: Rat brain tissue lysate

    Predicted band size: 140 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SIK3 antibody - C-terminal (ab211424), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SIK3 antibody - C-terminal (ab211424)

    Immunofluorescenct analysis of Rat brain cells labeling SIK3 with ab211424 at 20μg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK3 antibody - C-terminal (ab211424), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK3 antibody - C-terminal (ab211424)

    Immunohistochemical analysis of rormalin fixed, paraffin-embedded Human breast tissue labeling SIK3 with ab211424 at 5μg/ml.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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