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Rabbit Recombinant Monoclonal SIK3 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-SIK3 antibody [EPR23554-71] - BSA and Azide free (AB290756), expandable thumbnail
  • Western blot - Anti-SIK3 antibody [EPR23554-71] - BSA and Azide free (AB290756), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK3 antibody [EPR23554-71] - BSA and Azide free (AB290756), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK3 antibody [EPR23554-71] - BSA and Azide free (AB290756), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SIK3 antibody [EPR23554-71] - BSA and Azide free (AB290756), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Expected
Tested
Tested
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Tested
Rat
Expected
Expected
Tested
Not recommended
Expected

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Target data

Function

Positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor (By similarity). Required for chondrocyte hypertrophy during skeletogenesis (PubMed:22318228). Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3 (By similarity). Prevents HDAC4 translocation to the nucleus (PubMed:22318228).

Alternative names

Kiaa0999, Qsk, Sik3, Serine/threonine-protein kinase SIK3, Salt-inducible kinase 3, Serine/threonine-protein kinase QSK, SIK-3

Recommended products

Rabbit Recombinant Monoclonal SIK3 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR23554-71

Purification technique

Affinity purification Protein A

Specificity

This antibody does not react with Mouse and Rat species for IHC application.

This antibody does not react with Human species for ICC and FC application.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab290756 is a carrier free version of Anti-SIK3 antibody [EPR23554-71] ab255701.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SIK3 also known as salt-inducible kinase 3 is a serine/threonine-protein kinase with a molecular mass of approximately 140 kDa. This protein is expressed in many tissues including liver muscle and pancreas. Mechanically SIK3 phosphorylates key substrates involved in the regulation of various cellular functions enhancing or inhibiting their activity. Its action often involves regulating metabolism cytokine signaling and cellular growth processes.

Biological function summary

SIK3 participates in the regulation of glucose metabolism and energy homeostasis. It acts as part of larger complexes that control transcription factors and enzymes related to metabolic processes. These complexes help respond to cellular signals and external stress adjusting cellular functions to maintain balance. It influences the activity of enzymes and transcriptional regulators involved in lipid and carbohydrate metabolism playing a role in energy balance maintenance.

Pathways

SIK3 influences gluconeogenesis and insulin signaling pathways. It is closely connected with proteins such as AMPK (AMP-activated protein kinase) regulating energy sensor mechanisms and metabolic stress responses. These pathways are critical in controlling glucose production and utilization which affect overall energy homeostasis in the body.

Associated diseases and disorders

SIK3's dysfunction links to metabolic disorders like type 2 diabetes and obesity. Altered SIK3 activity affects insulin signaling which can contribute to impaired glucose regulation. It also connects to the protein LKB1 (liver kinase B1) in metabolic disease contexts as both play roles in regulating energy metabolism and maintaining glucose levels. Understanding SIK3's role provides insights for targeting metabolic diseases through therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com