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AB225634

Anti-SIK3 (phospho S551) antibody [EPR20878-12]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal SIK3 phospho S551 antibody. Suitable for ICC/IF, Dot, WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Kiaa0999, Qsk, Sik3, Serine/threonine-protein kinase SIK3, Salt-inducible kinase 3, Serine/threonine-protein kinase QSK, SIK-3

7 Images
Flow Cytometry (Intracellular) - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) cells transfected with flag-tagged SIK3 WT expression vector cells labelling SIK3 (phospho S551) with ab225634 at 1/500 dilution (0.1 μg) (Right) compared with a Rabbit monoclonal IgG isotype control (ab172730) (Left). A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells labelling SIK3 (phospho S551) with ab225634 at 1/100 (4.61 μg/mL) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody (ab150077) at 1/1000 (2 μg/mL) dilution (Green). Confocal image showing cytoplasmic staining in HEK-293T cells transfected with flag-tagged SIK3 WT expression vector, and no staining in HEK-293T cells transfected with flag-tagged SIK3 T551A expression vector. Anti-FLAG mouse monoclonal antibody was used to counterstain tubulin at 1/500 (2 μg/mL) dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 (2 μg/mL) dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling SIK3 (phospho S551) with ab225634 at 1/1000 (0.461 μg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebral cortex without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab225634 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Frozen sections) - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling SIK3 (phospho S551) with ab225634 at 1/100 (4.61 μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (Green). Positive staining on mouse cerebrum, and no staining has been shown after phosphatase treatment at 37°C for 2 hours. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Western blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634)
  • WB

Collaborator

Western blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634)

Blocking and diluting buffer and concentration : 3% BSA/TBST.

Data was kindly provided by Dr. Qinghua Liu, NIBS.

The 1st Ab working concentration was 5 μg/ml.

All lanes:

Western blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634) at 0.461 µg/mL

All lanes:

Mouse cortex tissue lysate at 4 µg

Predicted band size: 140 kDa,70 kDa

Observed band size: 150 kDa,70 kDa

false

Data was kindly provided by Dr. Qinghua Liu, NIBS.

Western blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634)
  • WB

Lab

Western blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTThis blot was developed using a higher sensitivity ECL substrate.

Exposure time : 3 minutes

All lanes:

Western blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634) at 1/1000 dilution

Lane 1:

Mouse hippocampus tissue lysate (Untreated membrane) at 10 µg

Lane 2:

Mouse hippocampus tissue lysate (Phosphatase treated membrane) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 140 kDa

Observed band size: 70 kDa

false

Dot Blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634)
  • Dot

Supplier Data

Dot Blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634)

Dot blot analysis of SIK3 (phospho S551) using ab225634 at 1/1000 (0.461 μg/mL) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

Lane 1 : SIK3 (phospho S551) peptide 1

Lane 2 : SIK3 (phospho S551) peptide 2

Lane 3 : SIK3 non-phospho peptide

Exposure time : 3 minutes.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

  • Carrier free

    Anti-SIK3 (phospho S551) antibody [EPR20878-12] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20878-12

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-Fr, Dot, ICC/IF, Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB255701

Anti-SIK3 antibody [EPR23554-71]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK3 antibody [EPR23554-71] (AB255701)

  • 0
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Primary Antibodies

AB225633

Anti-SIK3 (phospho T469) antibody [EPR20877-104]

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Flow Cytometry (Intracellular) - Anti-SIK3 (phospho T469) antibody [EPR20877-104] (AB225633)

  • 0
  • 0
Primary Antibodies

AB199474

Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196]

RabMAb|Recombinant

Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (AB199474)

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Primary Antibodies

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Anti-Ctip2 antibody [EPR23120-25]

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Flow Cytometry (Intracellular) - Anti-Ctip2 antibody [EPR23120-25] (AB240636)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/460", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIK3 also known as salt-inducible kinase 3 is a serine/threonine-protein kinase with a molecular mass of approximately 140 kDa. This protein is expressed in many tissues including liver muscle and pancreas. Mechanically SIK3 phosphorylates key substrates involved in the regulation of various cellular functions enhancing or inhibiting their activity. Its action often involves regulating metabolism cytokine signaling and cellular growth processes.
Biological function summary

SIK3 participates in the regulation of glucose metabolism and energy homeostasis. It acts as part of larger complexes that control transcription factors and enzymes related to metabolic processes. These complexes help respond to cellular signals and external stress adjusting cellular functions to maintain balance. It influences the activity of enzymes and transcriptional regulators involved in lipid and carbohydrate metabolism playing a role in energy balance maintenance.

Pathways

SIK3 influences gluconeogenesis and insulin signaling pathways. It is closely connected with proteins such as AMPK (AMP-activated protein kinase) regulating energy sensor mechanisms and metabolic stress responses. These pathways are critical in controlling glucose production and utilization which affect overall energy homeostasis in the body.

SIK3's dysfunction links to metabolic disorders like type 2 diabetes and obesity. Altered SIK3 activity affects insulin signaling which can contribute to impaired glucose regulation. It also connects to the protein LKB1 (liver kinase B1) in metabolic disease contexts as both play roles in regulating energy metabolism and maintaining glucose levels. Understanding SIK3's role provides insights for targeting metabolic diseases through therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor (By similarity). Required for chondrocyte hypertrophy during skeletogenesis (PubMed : 22318228). Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3 (By similarity). Prevents HDAC4 translocation to the nucleus (PubMed : 22318228).
See full target information Sik3 phospho S551
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 122:e2418317122 PubMed39847332

2025

Calcineurin governs baseline and homeostatic regulations of non-rapid eye movement sleep in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Yin,Zihan Zhang,Rui Zhou,Peng Zuo,Di Sang,Shuang Zhou,Bihan Shi,Lin Chen,Chongyang Wu,Ying Guo,Fengchao Wang,Eric Erquan Zhang,Qi Li,Masashi Yanagisawa,Qinghua Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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