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Rabbit Recombinant Monoclonal SIK3 phospho S551 antibody. Suitable for ICC/IF, Dot, WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse samples.

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Images

Western blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634), expandable thumbnail
  • Dot Blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPDotWBIHC-PIHC-FrFlow Cyt (Intra)
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Tested
Tested
Tested
Tested
Expected
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Rat, Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

1/460

Notes

-

Not recommended
Not recommended

Species

Rat, Human

Dilution info

-

Notes

-

Target data

Function

Positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor (By similarity). Required for chondrocyte hypertrophy during skeletogenesis (PubMed:22318228). Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3 (By similarity). Prevents HDAC4 translocation to the nucleus (PubMed:22318228).

Alternative names

Serine/threonine-protein kinase SIK3, Salt-inducible kinase 3, Serine/threonine-protein kinase QSK, SIK-3, Sik3, Qsk, Kiaa0999

Recommended products

Rabbit Recombinant Monoclonal SIK3 phospho S551 antibody. Suitable for ICC/IF, Dot, WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR20878-12

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SIK3 also known as salt-inducible kinase 3 is a serine/threonine-protein kinase with a molecular mass of approximately 140 kDa. This protein is expressed in many tissues including liver muscle and pancreas. Mechanically SIK3 phosphorylates key substrates involved in the regulation of various cellular functions enhancing or inhibiting their activity. Its action often involves regulating metabolism cytokine signaling and cellular growth processes.

Biological function summary

SIK3 participates in the regulation of glucose metabolism and energy homeostasis. It acts as part of larger complexes that control transcription factors and enzymes related to metabolic processes. These complexes help respond to cellular signals and external stress adjusting cellular functions to maintain balance. It influences the activity of enzymes and transcriptional regulators involved in lipid and carbohydrate metabolism playing a role in energy balance maintenance.

Pathways

SIK3 influences gluconeogenesis and insulin signaling pathways. It is closely connected with proteins such as AMPK (AMP-activated protein kinase) regulating energy sensor mechanisms and metabolic stress responses. These pathways are critical in controlling glucose production and utilization which affect overall energy homeostasis in the body.

Associated diseases and disorders

SIK3's dysfunction links to metabolic disorders like type 2 diabetes and obesity. Altered SIK3 activity affects insulin signaling which can contribute to impaired glucose regulation. It also connects to the protein LKB1 (liver kinase B1) in metabolic disease contexts as both play roles in regulating energy metabolism and maintaining glucose levels. Understanding SIK3's role provides insights for targeting metabolic diseases through therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634), expandable thumbnail

    Western blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634)

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTThis blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634) at 1/1000 dilution

    Lane 1: Mouse hippocampus tissue lysate (Untreated membrane) at 10 µg

    Lane 2: Mouse hippocampus tissue lysate (Phosphatase treated membrane) at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 140 kDa

    Observed band size: 70 kDa

    This data was developed using ab225634, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

  • Dot Blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634), expandable thumbnail

    Dot Blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634)

    Dot blot analysis of SIK3 (phospho S551) using ab225634 at 1/1000 (0.461 μg/mL) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100,000 dilution.

    Lane 1: SIK3 (phospho S551) peptide 1

    Lane 2: SIK3 (phospho S551) peptide 2

    Lane 3: SIK3 non-phospho peptide

    Exposure time: 3 minutes.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling SIK3 (phospho S551) with ab225634 at 1/1000 (0.461 μg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on mouse cerebral cortex without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab225634 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling SIK3 (phospho S551) with ab225634 at 1/100 (4.61 μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (Green). Positive staining on mouse cerebrum, and no staining has been shown after phosphatase treatment at 37°C for 2 hours. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunocytochemistry/ Immunofluorescence - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells labelling SIK3 (phospho S551) with ab225634 at 1/100 (4.61 μg/mL) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 (2 μg/mL) dilution (Green). Confocal image showing cytoplasmic staining in HEK-293T cells transfected with flag-tagged SIK3 WT expression vector, and no staining in HEK-293T cells transfected with flag-tagged SIK3 T551A expression vector. Anti-FLAG mouse monoclonal antibody was used to counterstain tubulin at 1/500 (2 μg/mL) dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 (2 μg/mL) dilution.

  • Flow Cytometry (Intracellular) - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) cells transfected with flag-tagged SIK3 WT expression vector cells labelling SIK3 (phospho S551) with ab225634 at 1/500 dilution (0.1 μg) (Right) compared with a Rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left). A Goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634), expandable thumbnail
    Data was kindly provided by Dr. Qinghua Liu, NIBS.

    Western blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634)

    Blocking and diluting buffer and concentration: 3% BSA/TBST.

    Data was kindly provided by Dr. Qinghua Liu, NIBS.

    The 1st Ab working concentration was 5 μg/ml.

    All lanes: Western blot - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (ab225634) at 0.461 µg/mL

    All lanes: Mouse cortex tissue lysate at 4 µg

    Predicted band size: 140 kDa, 70 kDa

    Observed band size: 150 kDa, 70 kDa

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Product protocols

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