JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB283509

Anti-SIK3 (phospho T469) antibody [EPR20877-104] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal SIK3 phospho T469 antibody. Carrier free. Suitable for Dot, IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Synthetic peptide - Mouse, Mouse, Transfected cell line samples.
6 Images
Flow Cytometry (Intracellular) - Anti-SIK3 (phospho T469) antibody [EPR20877-104] - BSA and Azide free (AB283509)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SIK3 (phospho T469) antibody [EPR20877-104] - BSA and Azide free (AB283509)

This data was developed using ab225633, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) cells transfected with flag-tagged SIK3 WT expression vector cells labelling SIK3 (phospho T469) with ab225633 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG isotype control (ab172730) (Left). A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-SIK3 (phospho T469) antibody [EPR20877-104] - BSA and Azide free (AB283509)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SIK3 (phospho T469) antibody [EPR20877-104] - BSA and Azide free (AB283509)

This data was developed using ab225633, the same antibody clone in a different buffer formulation.

Immunocytochemical analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells labelling SIK3 (phospho T469) with ab225633 at 1/100 (5.22 ug/mL) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody (ab150077) at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with flag-tagged SIK3 WT expression vector, and no staining in 293T cells transfected with flag-tagged SIK3 T469A expression vector is observed. Anti-FLAG mouse monoclonal antibody was used to counterstain tubulin at 1/500 (2 ug/mL) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 (2 ug/mL) dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK3 (phospho T469) antibody [EPR20877-104] - BSA and Azide free (AB283509)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK3 (phospho T469) antibody [EPR20877-104] - BSA and Azide free (AB283509)

This data was developed using ab225633, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling SIK3 (phospho T469) with ab225633 at 1/1000 (0.522 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebral cortex without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab225633 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Western blot - Anti-SIK3 (phospho T469) antibody [EPR20877-104] - BSA and Azide free (AB283509)
  • WB

Lab

Western blot - Anti-SIK3 (phospho T469) antibody [EPR20877-104] - BSA and Azide free (AB283509)

This data was developed using ab225633, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Data was kindly provided by Dr. Qinghua Liu, NIBS.

The 1st Ab working concentration was 5 ug/ml.

Exposure time :

All lanes:

Western blot - Anti-SIK3 (phospho T469) antibody [EPR20877-104] (<a href='/en-us/products/primary-antibodies/sik3-phospho-t469-antibody-epr20877-104-ab225633'>ab225633</a>) at 0.522 mg/mL

All lanes:

Mouse cortex tissue lysate at 4 µg

Predicted band size: 140 kDa

Observed band size: 150 kDa,70 kDa

false

Western blot - Anti-SIK3 (phospho T469) antibody [EPR20877-104] - BSA and Azide free (AB283509)
  • WB

Lab

Western blot - Anti-SIK3 (phospho T469) antibody [EPR20877-104] - BSA and Azide free (AB283509)

This data was developed using ab225633, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 114 seconds.

All lanes:

Western blot - Anti-SIK3 (phospho T469) antibody [EPR20877-104] (<a href='/en-us/products/primary-antibodies/sik3-phospho-t469-antibody-epr20877-104-ab225633'>ab225633</a>) at 1/5000 dilution

Lane 1:

Mouse hippocampus tissue lysate (Untreated membrane) at 10 µg

Lane 2:

Mouse hippocampus tissue lysate (Phosphatase treated membrane) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 140 kDa

Observed band size: 70 kDa

false

Dot Blot - Anti-SIK3 (phospho T469) antibody [EPR20877-104] - BSA and Azide free (AB283509)
  • Dot

Supplier Data

Dot Blot - Anti-SIK3 (phospho T469) antibody [EPR20877-104] - BSA and Azide free (AB283509)

This data was developed using ab225633, the same antibody clone in a different buffer formulation.

Dot blot analysis of SIK3 (phospho T469) using ab225633 at 1/1000 (0.522 ug/mL) dilution followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.

Lane 1 : SIK3 (phospho T469) peptide

Lane 2 : SIK3 non-phospho peptide

Exposure time : 3 minutes.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

  • Unconjugated

    Anti-SIK3 (phospho T469) antibody [EPR20877-104]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20877-104

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Dot, WB, IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB225634

Anti-SIK3 (phospho S551) antibody [EPR20878-12]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-SIK3 (phospho S551) antibody [EPR20878-12] (AB225634)

  • 0
  • 0
Primary Antibodies

AB255701

Anti-SIK3 antibody [EPR23554-71]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK3 antibody [EPR23554-71] (AB255701)

  • 0
  • 0
Primary Antibodies

AB199474

Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196]

RabMAb|Recombinant

Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (AB199474)

  • 5
  • 3
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

KD Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Synthetic peptide - Mouse": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

Product details

ab283509 is the carrier-free version of ab225633.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIK3 also known as salt-inducible kinase 3 is a serine/threonine-protein kinase with a molecular mass of approximately 140 kDa. This protein is expressed in many tissues including liver muscle and pancreas. Mechanically SIK3 phosphorylates key substrates involved in the regulation of various cellular functions enhancing or inhibiting their activity. Its action often involves regulating metabolism cytokine signaling and cellular growth processes.
Biological function summary

SIK3 participates in the regulation of glucose metabolism and energy homeostasis. It acts as part of larger complexes that control transcription factors and enzymes related to metabolic processes. These complexes help respond to cellular signals and external stress adjusting cellular functions to maintain balance. It influences the activity of enzymes and transcriptional regulators involved in lipid and carbohydrate metabolism playing a role in energy balance maintenance.

Pathways

SIK3 influences gluconeogenesis and insulin signaling pathways. It is closely connected with proteins such as AMPK (AMP-activated protein kinase) regulating energy sensor mechanisms and metabolic stress responses. These pathways are critical in controlling glucose production and utilization which affect overall energy homeostasis in the body.

SIK3's dysfunction links to metabolic disorders like type 2 diabetes and obesity. Altered SIK3 activity affects insulin signaling which can contribute to impaired glucose regulation. It also connects to the protein LKB1 (liver kinase B1) in metabolic disease contexts as both play roles in regulating energy metabolism and maintaining glucose levels. Understanding SIK3's role provides insights for targeting metabolic diseases through therapeutic strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor. Involved in the dynamic regulation of mTOR signaling in chondrocyte differentiation during skeletogenesis (PubMed : 30232230). Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3 (Probable). Prevents HDAC4 translocation to the nucleus (By similarity).
See full target information SIK3 pT469

Alternative Names

KIAA0999, QSK, L19, SIK3, Serine/threonine-protein kinase SIK3, Salt-inducible kinase 3, Serine/threonine-protein kinase QSK, SIK-3
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com